Human HES-1 Antibody Summary
Accession # Q14469
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human HES‑1 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected or transfected with human HES-1. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human HES‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3317) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for HES‑1 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
HES‑1 in Human Ovarian Cancer Tissue. HES‑1 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Goat Anti-Human HES‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3317) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
HES-1 is a transcriptional repressor that is a target of notch signaling. It contains a basic helix-loop-helix (bHLH) DNA-binding domain, an Orange domain and a C‑terminal tetrapeptide WRPW motif that binds to the Groucho (Gro)/TLE/Grg family of corepressors. HES-1 can form both homo- and heterodimers with other HES family members. Dimerization is mediated through both the bHLH and the downstream Orange domain. Over the sequence used for immunization, human HES-1 shares 97% and 98% amino acid sequence identity with mouse and rat HES-1, respectively.
Citations for Human HES-1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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Interplay between CCR7 and Notch1 axes promotes stemness in MMTV-PyMT mammary cancer cells
Authors: ST Boyle, KA Gieniec, CE Gregor, JW Faulkner, SR McColl, M Kochetkova
Mol. Cancer, 2017;16(1):19.
Sample Types: Whole Cells
Applications: Western Blot
Rabconnectin-3 is a functional regulator of mammalian Notch signaling.
Authors: Sethi N, Yan Y, Quek D
J. Biol. Chem., 2010;285(45):34757-64.
Sample Types: Whole Cells
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