Human HES-1 Antibody

Catalog # Availability Size / Price Qty
AF3317
AF3317-SP
Detection of Human HES‑1 by Western Blot.
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Product Details
Citations (2)
FAQs
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Human HES-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human HES-1 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant human (rh) HES‑2, rhHES-4, rhHES-5, rhHES-6, and rhHES-7 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human HES-1
Arg93-Asn280
Accession # Q14469
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human HES‑1 by Western Blot. View Larger

Detection of Human HES‑1 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected or transfected with human HES-1. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human HES‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3317) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for HES‑1 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunohistochemistry HES‑1 in Human Ovarian Cancer Tissue. View Larger

HES‑1 in Human Ovarian Cancer Tissue. HES‑1 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Goat Anti-Human HES‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3317) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HES-1

HES-1 is a transcriptional repressor that is a target of notch signaling. It contains a basic helix-loop-helix (bHLH) DNA-binding domain, an Orange domain and a C‑terminal tetrapeptide WRPW motif that binds to the Groucho (Gro)/TLE/Grg family of corepressors. HES-1 can form both homo- and heterodimers with other HES family members. Dimerization is mediated through both the bHLH and the downstream Orange domain. Over the sequence used for immunization, human HES-1 shares 97% and 98% amino acid sequence identity with mouse and rat HES-1, respectively.

Long Name
Hairy And Enhancer of Split 1
Entrez Gene IDs
3280 (Human); 15205 (Mouse); 29577 (Rat)
Alternate Names
BHLHB39; bHLHb39hairy homolog (Drosophila); Class B basic helix-loop-helix protein 39; FLJ20408; Hairy and enhancer of split 1; hairy and enhancer of split 1, (Drosophila); Hairy homolog; Hairy-like protein; HES1; HES-1; hHL; HL; HRY; HRYHHL; transcription factor HES-1

Product Datasheets

Citations for Human HES-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Interplay between CCR7 and Notch1 axes promotes stemness in MMTV-PyMT mammary cancer cells
    Authors: ST Boyle, KA Gieniec, CE Gregor, JW Faulkner, SR McColl, M Kochetkova
    Mol. Cancer, 2017;16(1):19.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: WB
  2. Rabconnectin-3 is a functional regulator of mammalian Notch signaling.
    Authors: Sethi N, Yan Y, Quek D
    J. Biol. Chem., 2010;285(45):34757-64.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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Secondary Antibodies

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