Human HES-1 Antibody

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Detection of Human HES‑1 by Western Blot.
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Product Details
Citations (3)
Supplemental Products

Human HES-1 Antibody Summary

Species Reactivity
Detects human HES-1 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant human (rh) HES‑2, rhHES-4, rhHES-5, rhHES-6, and rhHES-7 is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant human HES-1
Accession # Q14469
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human HES-1 antibody by Western Blot. View Larger

Detection of Human HES‑1 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected or transfected with human HES-1. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human HES-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3317) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for HES-1 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunohistochemistry HES‑1 antibody in Human Ovarian Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

HES‑1 in Human Ovarian Cancer Tissue. HES-1 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Goat Anti-Human HES-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3317) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HES-1

HES-1 is a transcriptional repressor that is a target of notch signaling. It contains a basic helix-loop-helix (bHLH) DNA-binding domain, an Orange domain and a C‑terminal tetrapeptide WRPW motif that binds to the Groucho (Gro)/TLE/Grg family of corepressors. HES-1 can form both homo- and heterodimers with other HES family members. Dimerization is mediated through both the bHLH and the downstream Orange domain. Over the sequence used for immunization, human HES-1 shares 97% and 98% amino acid sequence identity with mouse and rat HES-1, respectively.

Long Name
Hairy And Enhancer of Split 1
Entrez Gene IDs
3280 (Human); 15205 (Mouse); 29577 (Rat)
Alternate Names
BHLHB39; bHLHb39hairy homolog (Drosophila); Class B basic helix-loop-helix protein 39; FLJ20408; Hairy and enhancer of split 1; hairy and enhancer of split 1, (Drosophila); Hairy homolog; Hairy-like protein; HES1; HES-1; hHL; HL; HRY; HRYHHL; transcription factor HES-1

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Citations for Human HES-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Decreased p53 is associated with a decline in asymmetric stem cell self-renewal in aged human epidermis
    Authors: A Charruyer, T Weisenberg, H Li, A Khalifa, AW Schroeder, A Belzer, R Ghadially
    Aging Cell, 2021-02-01;0(0):e13310.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. Interplay between CCR7 and Notch1 axes promotes stemness in MMTV-PyMT mammary cancer cells
    Authors: ST Boyle, KA Gieniec, CE Gregor, JW Faulkner, SR McColl, M Kochetkova
    Mol. Cancer, 2017-01-31;16(1):19.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Western Blot
  3. Rabconnectin-3 is a functional regulator of mammalian Notch signaling.
    Authors: Sethi N, Yan Y, Quek D
    J. Biol. Chem., 2010-09-01;285(45):34757-64.
    Species: Human, Mouse
    Sample Types: Whole Cells
    Applications: ICC


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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Goat IgG (H+L) Antibody


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