HGF R, also known as Met (from N-methyl-N’-nitro-N-nitrosoguanidine induced), is a glycosylated receptor tyrosine kinase that plays a central role in epithelial morphogenesis and cancer development. HGF R is synthesized as a single chain precursor which undergoes cotranslational proteolytic cleavage. This generates a mature HGF R that is a disulfide-linked dimer composed of a 50 kDa extracellular alpha chain and a 145 kDa transmembrane beta chain (1, 2). The extracellular domain (ECD) contains a seven bladed beta -propeller sema domain, a cysteine-rich PSI/MRS, and four Ig-like E-set domains, while the cytoplasmic region includes the tyrosine kinase domain (3, 4). Proteolysis and alternate splicing generate additional forms of human HGF R which either lack of the kinase domain, consist of secreted extracellular domains, or are deficient in proteolytic separation of the alpha and beta chains (5-7). The sema domain, which is formed by both the alpha and beta chains of HGF R, mediates both ligand binding and receptor dimerization (3, 8). Ligand-induced tyrosine phosphorylation in the cytoplasmic region activates the kinase domain and provides docking sites for multiple SH2-containing molecules (9, 10). HGF stimulation induces HGF R downregulation via internalization and proteasome-dependent degradation (11). In the absence of ligand, HGF R forms non-covalent complexes with a variety of membrane proteins including CD44v6, CD151, EGF R, Fas, Integrin alpha 6/ beta 4, Plexins B1, 2, 3, and MSP R/Ron (12-19). Ligation of one complex component triggers activation of the other, followed by cooperative signaling effects (12-19). Formation of some of these heteromeric complexes is a requirement for epithelial cell morphogenesis and tumor cell invasion (12, 16, 17). Paracrine induction of epithelial cell scattering and branching tubulogenesis results from the stimulation of HGF R on undifferentiated epithelium by HGF released from neighboring mesenchymal cells (20). Genetic polymorphisms, chromosomal translocation, over-expression, and additional splicing and proteolytic cleavage of HGF R have been described in a wide range of cancers (1). Within the ECD, human HGF R shares 86-88% amino acid sequence identity with canine, mouse, and rat HGF R.
Human HGFR/c-MET Antibody (95106)
R&D Systems | Catalog # MAB3582
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Flow Cytometry, CyTOF-ready
Cited:
Flow Cytometry, Immunocytochemistry, Western Blo
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 95106
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human HGF R/c-MET
Glu25-Thr932
Accession # P08581
Glu25-Thr932
Accession # P08581
Specificity
Detects human HGF R/c-MET.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human HGFR/c-MET Antibody (95106)
Detection of HGF R/c‑MET in MDA‑MB‑231 Human Cell Line by Flow Cytometry.
MDA-MB-231 human breast cancer cell line was stained with Human HGF R/c-MET Monoclonal Antibody (Catalog # MAB3582, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B).
Detection of HGFR/c-MET by Western Blot
Generation of CARs and analysis of IL-2 secretion by c-Met CAR Jurkat cell. (A) Schematic representation of c-Met CAR and FITC CAR construct. (B) c-Met gene (pCMV3-Met) was transduced by electroporation into K562, and Western blot was performed. (C) After transducing the mock CD8sp-c-Met CAR, CSF2Rsp-c-Met CAR, or FITC CAR construct into Jurkat, expressions of CAR were confirmed through Western blot. (D) The mock CD8sp-c-Met CAR, CSF2Rsp-c-Met CAR, or FITC CAR Jurkat were co-cultured with K562 or c-Met-K562 at an E:T ratio of 15:1. After overnight incubation, supernatants were collected and ELISA was performed to measure IL-2 level. (The whole western blots figure see Figure S1). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34830894), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of HGFR/c-MET by Western Blot
c-Met CAR KHYG-1 specifically lyses the c-Met positive GC cells. (A) Western blot with the cell lysates of mock CD8sp-c-Met, CSF2Rsp-c-Met, or FITC KHYG-1 cells to see the CAR expression (B) The mock CD8sp-c-Met CAR, CSF2Rsp-c-Met CAR, or FITC CAR KHYG-1 were co-incubated with MKN-45, SNU-5, SNU-1, and SNU-484 at E:T ratio of 5:1 or 10:1 for 5 h. The cytotoxicity of CAR KHYG-1 was measured by the Bright-Glo (luciferase) assay system. (The whole western blots figure see Figure S1). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34830894), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Human HGFR/c-MET Antibody (95106)
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: MDA‑MB‑231 human breast cancer cell line
Sample: MDA‑MB‑231 human breast cancer cell line
Reviewed Applications
Read 4 reviews rated 4 using MAB3582 in the following applications:
Flow Cytometry Panel Builder
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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: HGFR/c-MET
References
- Birchmeier, C. et al. (2003) Nat. Rev. Mol. Cell Biol. 4:915.
- Corso, S. et al. (2005) Trends Mol. Med. 11:284.
- Gherardi, E. et al. (2003) Proc. Natl. Acad. Sci. USA 100:12039.
- Park, M. et al. (1987) Proc. Natl. Acad. Sci. USA 84:6379.
- Crepaldi, T. et al. (1994) J. Biol. Chem. 269:1750.
- Prat, M. et al. (1991) Mol. Cell. Biol. 12:5954.
- Rodrigues, G.A. et al. (1991) Mol. Cell. Biol. 11:2962.
- Kong-Beltran, M. et al. (2004) Cancer Cell 6:75.
- Naldini, L. et al. (1991) Mol. Cell. Biol. 11:1793.
- Ponzetto, C. et al. (1994) Cell 77:261.
- Jeffers, M. et al. (1997) Mol. Cell. Biol. 17:799.
- Orian-Rousseau, V. et al. (2002) Genes Dev. 16:3074.
- Klosek, S.K. et al. (2005) Biochem. Biophys. Res. Commun. 336:408.
- Jo, M. et al. (2000) J. Biol. Chem. 275:8806.
- Wang, X. et al. (2002) Mol. Cell 9:411.
- Trusolino, L. et al. (2001) Cell 107:643.
- Giordano, S. et al. (2002) Nat. Cell Biol. 4:720.
- Conrotto, P. et al. (2004) Oncogene 23:5131.
- Follenzi, A. et al. (2000) Oncogene 19:3041.
- Sonnenberg, E. et al. (1993) J. Cell Biol. 123:223.
Long Name
Hepatocyte Growth Factor Receptor
Alternate Names
c-MET, cMET, HGF R, MET
Gene Symbol
MET
UniProt
Additional HGFR/c-MET Products
Product Documents for Human HGFR/c-MET Antibody (95106)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human HGFR/c-MET Antibody (95106)
For research use only
Citations for Human HGFR/c-MET Antibody (95106)
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Customer Reviews for Human HGFR/c-MET Antibody (95106) (4)
4 out of 5
4 Customer Ratings
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Application: MicroarraysSample Tested: EDTA PlasmaSpecies: HumanVerified Customer | Posted 01/14/2021
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Application: Flow CytometrySample Tested: A-431 epidermoid carcinoma cell lineSpecies: HumanVerified Customer | Posted 12/23/2020Detection of human HGFR/c-MET on epidermoid carcinoma cell line A-431. A-431 cells were treated with 100 nM of the human HGFR/c-MET antibody (catalog # MAB3582) or mouse IgG1 isotype control, followed by a secondary antibody goat a-mouse IgG Fc APC. Isotype control (RED), human HGFR/c-MET antibody (BLUE).
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Application: MicroarraySample Tested: EDTA PlasmaSpecies: HumanVerified Customer | Posted 02/08/2020Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma
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Application: MicroarraysSample Tested: EDTA PlasmaSpecies: HumanVerified Customer | Posted 11/14/2018
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- Flow Cytometry Troubleshooting Guide
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- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Liperfluo
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- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
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- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Human HGFR/c-MET Antibody (95106)
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Q: Does Human HGFR/c-MET Antibody, Catalog # MAB3582, bind to the alpha chain or beta chain of HGFR/c-MET?
A: The immunogen for MAB3582 (Recombinant human HGF R/cMET, aa Glu25-Thr932) spans both the alpha and beta chain regions of Human HGFR and we do not epitope map our antibodies. However, staining in flow cytometry detects a surface epitope. Since only the alpha chain is extracellular in location and the beta chain is transmembrane in location, the antibody likley binds the alpha chain of this protein.
