Human Histamine H3 R Antibody Summary
Accession # Q9Y5N1
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Histamine H3 R in HEK293 Human Cell Line Transfected with Human Histamine H3 R and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) Histamine H3 R or (B) irrelevant protein, and eGFP were stained with Mouse Anti-Human Histamine H3 R Monoclonal Antibody (Catalog # MAB10200) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based Mouse IgG2B Isotype Control Antibody staining (Catalog # MAB0041, data not shown). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Histamine H3 R
Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors Histamine H1 R, Histamine H2 R, Histamine H3 R and Histamine H4 R. HRH3 encodes one of the histamine receptors (H3) which belongs to the family 1 of G protein-coupled receptors. Histamine H3 R is an integral membrane protein and can regulate neurotransmitter release. It can also increase voltage-dependent calcium current in smooth muscles and innervates the blood vessels and the heart in cardiovascular system.
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