Human HOXB7 Antibody

Catalog # Availability Size / Price Qty
MAB80401
MAB80401-SP
Detection of Human HOXB7 by Western Blot.
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Human HOXB7 Antibody Summary

Species Reactivity
Human
Specificity
Detects human HOXB7 in direct ELISAs and Western blots. In direct ELISAa, no cross-reactivity with recombinant human HOXA7 is observed.
Source
Monoclonal Mouse IgG2B Clone # 864617
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human HOXB7
Ser2-Asn123
Accession # P09629
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human HOXB7 antibody by Western Blot. View Larger

Detection of Human HOXB7 by Western Blot. Western blot shows lysates of HCT-116 human colorectal carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human HOXB7 Monoclonal Antibody (Catalog # MAB80401) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HOXB7 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HOXB7

HOXB7 (Homeobox protein B7), also called Hox-2C, is a 24 kDa (predicted) member of the Antp homeobox family of transcription factors. It is widely expressed, and is overexpressed in many cancers such as melanoma and tumors in lung and breast. It promotes tumorigenesis by promoting activities such as proliferation, invasion, epithelial-mesenchymal transition and angiogenesis.  Human HOXB7 is a 217 amino acid (aa) protein with an Antp-type hexapeptide (aa 126-131) that mediates heterodimerization, and a DNA-binding homeobox domain (aa 137-196). Human HOXB7 aa 1-123 shares 92% aa sequence identity with mouse and rat HOXB7.

Long Name
Homeobox B7
Entrez Gene IDs
3217 (Human); 15415 (Mouse); 497985 (Rat)
Alternate Names
HHO.C1; homeo box 2C; homeo box B7; homeo box c1 protein; homeobox B7; Homeobox protein HHO.C1; Homeobox protein Hox-2C; homeobox protein Hox-B7; HOX2; Hox-2.3; HOX2C; HOX2CHox-2.3; HOXB7

Product Datasheets

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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