Human HOXB7 Antibody Summary
Accession # P09629
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human HOXB7 by Western Blot. Western blot shows lysates of HCT-116 human colorectal carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human HOXB7 Monoclonal Antibody (Catalog # MAB80401) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HOXB7 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
HOXB7 (Homeobox protein B7), also called Hox-2C, is a 24 kDa (predicted) member of the Antp homeobox family of transcription factors. It is widely expressed, and is overexpressed in many cancers such as melanoma and tumors in lung and breast. It promotes tumorigenesis by promoting activities such as proliferation, invasion, epithelial-mesenchymal transition and angiogenesis. Human HOXB7 is a 217 amino acid (aa) protein with an Antp-type hexapeptide (aa 126-131) that mediates heterodimerization, and a DNA-binding homeobox domain (aa 137-196). Human HOXB7 aa 1-123 shares 92% aa sequence identity with mouse and rat HOXB7.
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