Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 864617
View all HOXB7 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human HOXB7
Ser2-Asn123
Accession # P09629

Specificity

Detects human HOXB7 in direct ELISAs and Western blots. In direct ELISAa, no cross-reactivity with recombinant human HOXA7 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human HOXB7 Antibody

Detection of Human HOXB7 antibody by Western Blot.

Detection of Human HOXB7 by Western Blot.

Western blot shows lysates of HCT-116 human colorectal carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human HOXB7 Monoclonal Antibody (Catalog # MAB80401) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HOXB7 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human HOXB7 Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: HCT‑116 human colorectal carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HOXB7

HOXB7 (Homeobox protein B7), also called Hox-2C, is a 24 kDa (predicted) member of the Antp homeobox family of transcription factors. It is widely expressed, and is overexpressed in many cancers such as melanoma and tumors in lung and breast. It promotes tumorigenesis by promoting activities such as proliferation, invasion, epithelial-mesenchymal transition and angiogenesis.  Human HOXB7 is a 217 amino acid (aa) protein with an Antp-type hexapeptide (aa 126-131) that mediates heterodimerization, and a DNA-binding homeobox domain (aa 137-196). Human HOXB7 aa 1-123 shares 92% aa sequence identity with mouse and rat HOXB7.

Long Name

Homeobox B7

Alternate Names

HHO.C1, Hox-2.3, HOX2C

Entrez Gene IDs

3217 (Human); 15415 (Mouse); 497985 (Rat)

Gene Symbol

HOXB7

UniProt

P09629

Additional HOXB7 Products

Product Documents for Human HOXB7 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human HOXB7 Antibody

For research use only

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