Human HOXD10 Antibody Summary
Accession # P28358
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
HOXD10 in Human iPS Cells Differentiated to NPCs. HOXD10 was detected in immersion fixed human induced pluripotent stem (iPS) cells differentiated to neural progenitor cells (NPC) using Goat Anti-Human HOXD10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF10413) at 10 µg/mL, and co-stained with Mouse Anti-Human Nestin Monoclonal Antibody (Catalog # MAB1259) at 10 µg/mL, for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001), NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; Catalog # NL009) and counterstained with DAPI (blue). Specific staining for HOXD10 was localized to nucleus, while Nestin co-staining was cytoplasmic. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
HOXD10 is a sequence-specific transcription factor that belongs to the Abd-B homeobox family. It is a nuclear protein that is expressed in developing limb buds and is involved in differentiation and limb development. HOXD10 mutations have been associated with foot deformities such as rocker-bottom foot and the deformity seen in Charcot-Marie-Tooth disease. HOXD10 also functions as a candidate tumor suppressor. In gastric cancer there is downregulation in gastric cancer tissues and cell lines relative to normal stomach tissues. It has also been reported to suppress ovarian cancer, hepatocellular carcinoma and other cancer cells. Re-expression of HOXD10 results in significant inhibition of cell survival, induction of cell apoptosis and impairment of cell migration and invasion.
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