Human ICA1 Antibody Summary
Accession # ABW03414
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of ICA1 in A172 Human Cell Line by Flow Cytometry. A172 human glioblastoma cell line was stained with Goat Anti-Human ICA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4084, filled histo-gram) or isotype control antibody (AB-108-C, open histo-gram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (F0107). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
ICA1 in paraffin-embedded human pancreas. ICA1 was detected in immersion fixed paraffin-embedded sections of human pancreas using Goat Anti-Human ICA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4084) at 0.5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (red; Catalog # VC004) and counterstained with hematoxylin (blue). Specific staining was localized to islets of Langerhans.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Islet Cell Autoantigen 1 (ICA1), also known as ICA 69 kDa (ICA69), is a cytosolic protein that is associated with the Golgi complex and with immature secretory granules. Its N-terminal region shares similarity with arfaptins (ADP-ribosylation factor-interacting-proteins), suggesting that ICA1 likely plays a role in membrane trafficking. Human ICA-1 is a 483 amino acids (aa) protein. As a result of aa insertions, deletions and substitutions, multiple isoforms exist. Human ICA-1 shows 89% and 94% aa identity to mouse and canine ICA-1, respectively.
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