Human ICA1 Antibody

Catalog # Availability Size / Price Qty
AF4084
AF4084-SP
Detection of ICA1 in A172 Human Cell Line by Flow Cytometry.
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Human ICA1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human ICA1 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human ICA1
Ser2-Ala482
Accession # ABW03414
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human ICA1
Immunohistochemistry
5-15 µg/mL
Immersion fixed paraffin-embedded sections of human pancreatic cancer tissue subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Intracellular Staining by Flow Cytometry Detection of ICA1 antibody in A172 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of ICA1 in A172 Human Cell Line by Flow Cytometry. A172 human glioblastoma cell line was stained with Goat Anti-Human ICA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4084, filled histo-gram) or isotype control antibody (AB-108-C, open histo-gram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (F0107). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Immunohistochemistry View Larger

ICA1 in paraffin-embedded human pancreas. ICA1 was detected in immersion fixed paraffin-embedded sections of human pancreas using Goat Anti-Human ICA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4084) at 0.5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (red; Catalog # VC004) and counterstained with hematoxylin (blue). Specific staining was localized to islets of Langerhans.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ICA1

Islet Cell Autoantigen 1 (ICA1), also known as ICA 69 kDa (ICA69), is a cytosolic protein that is associated with the Golgi complex and with immature secretory granules. Its N-terminal region shares similarity with arfaptins (ADP-ribosylation factor-interacting-proteins), suggesting that ICA1 likely plays a role in membrane trafficking. Human ICA-1 is a 483 amino acids (aa) protein. As a result of aa insertions, deletions and substitutions, multiple isoforms exist. Human ICA-1 shows 89% and 94% aa identity to mouse and canine ICA-1, respectively.

Long Name
Islet Cell Autoantigen 1
Entrez Gene IDs
3382 (Human)
Alternate Names
diabetes mellitus type I autoantigen; ICA1; ICA69; ICAp69; ICAp69Islet cell autoantigen p69,69 kDa islet cell autoantigen; islet cell autoantigen 1 (69kD); islet cell autoantigen 1 isoform; islet cell autoantigen 1; islet cell autoantigen 1, 69kDa; p69

Product Datasheets

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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