Human IFN-alpha 6/IFNA6 Antibody

Catalog # Availability Size / Price Qty
MAB10212-100
MAB10212-SP
Human IFN-alpha 6/IFNA6 Antibody in Western Blot
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Human IFN-alpha 6/IFNA6 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IFN-alpha 6/IFNA6 in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG2A Clone # 1009720
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human IFN-alpha 6/IFNA6
Leu28-Lys207
Accession # Q90W0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection  of Human IFN-alpha 6/IFNA6 by Western Blot. View Larger

Detection of Human IFN-alpha 6/IFNA6 by Western Blot. Western blot shows lysates of HEK001 human epidermal keratinocyte cell line and NHEK human normal epidermal keratinocytes. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human IFN-alpha 6/IFNA6 Monoclonal Antibody (Catalog # MAB10212) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IFN-alpha 6/IFNA6 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-alpha 6/IFNA6

Interferon kappa, also known as IFN-kappa, is a 207 aminoacids protein that in humans is encoded by the IFNK gene. IFN-kappa is a member of the type I interferon family which also includes IFN-alpha, -beta, -epsilon, and -omega. Type I interferons are a group of related glycoproteins that play an important role in host defenses against viral infections. Human IFN-kappa has been detected in keratinocytes, monocytes, and monocyte-derived dendritic cells and is reported to have contact-dependent antiviral activity. Human papillomavirus (HPV) 16 oncogene expression, which is necessary for the development of cervical cancer, has been shown to down-regulate human IFN-kappa expression.

Long Name
Interferon alpha 6
Entrez Gene IDs
3443 (Human); 15969 (Mouse)
Alternate Names
IFNA6; IFN-alpha 6; IFNalpha K; IFN-alpha K; IFN-kappa; interferon kappa; interferon, kappa; RP11-27J8.1; UNQ6124/PRO20084

Product Datasheets

FAQs

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Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

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