Human IFN-alpha Antibody

Catalog #: 21100-1
6 Citations Datasheet / COA / SDS

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21100-1

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Citations (6)

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Human IFN-alpha Antibody Summary

Species Reactivity

Human

Specificity

Neutralizes human interferon alpha; binds to human interferon alpha with high affinity (K_A ≈ 10⁹ M^-1)

Source

Monoclonal Mouse IgG₁ Clone # MMHA-2

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human Leukocyte Interferon

Formulation

Phosphate-buffered saline (PBS) containing 0.1% bovine serum albumin (BSA).

Endotoxin Level

<1.0 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilutions should be determined by each laboratory for each application.

ELISA

Optimal dilutions should be determined by each laboratory for each application.

Neutralization

One neutralization unit is the amount of antiserum required to neutralize one unit of human interferon alpha (Hu-IFN-alpha ) to a 50% endpoint. Interferon was titrated with the use of the cytopathic effect inhibition assay as described [Rubinstein, S., Famille tti, P.C., and Pestka, S. (1981) "Convenient Assay for Interferons," J. Virol. 37, 755-758; Familletti, P.C., Rubinstein, S., and Pestka, S. (1981) "A Convenient and Rapid Cytopathic Effect Inhibition Assay for Interferon," in Methods in Enzymology, Vol. 78 (S. Pestka, ed.), Academic Press, New York, 387-394]. In this antiviral assay for interferon, about 1 unit/ml of interferon is the quantity necessary to produce a cytopathic effect of 50%. The units are determined with respect to the international reference standard for Hu-IFN-alpha provided by the National Institutes of Health [see Pestka, S. (1986) "Interferon Standards and General Abbreviations," in Methods in Enzymology (S. Pestka, ed.), Academic Press, New York 119, 14-23].

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

After receipt, the product may be stored at -20°C for short-term use ( ≤ 6 months). For long-term storage, we recommend storing the product at -70°C or below for retention of full activity. When thawing, the contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum. Re-freezing should be done on dry ice or in a dry ice/alcohol bath. Further dilution of the product should be in buffers containing protein such as 0.1% bovine serum albumin (BSA).

Background: IFN-alpha

Interferon-alpha (IFN-alpha), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R2) and a 125 kDa ligand binding and signal transduction subunit (IFN-alpha R1) that is involved both in ligand binding and signal transduction. IFN-alpha has both anti-viral and immunomodulatory activities on target cells.

Long Name

Interferon alpha

Entrez Gene IDs

3440 (Human)

Alternate Names

IFNA; IFNA2; IFNA2b; IFNalpha; IFN-alpha; IFN-alphaA; INFA2; interferon alpha 2; LeIF D

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Citations for Human IFN-alpha Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Antigen delivery to dendritic cells shapes human CD4+ and CD8+ T cell memory responses to Staphylococcus aureus
Authors: J Uebele, C Stein, MT Nguyen, A Schneider, F Kleinert, O Tichá, G Bierbaum, F Götz, I Bekeredjia
PLoS Pathog., 2017-05-25;13(5):e1006387.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
Constitutively Active MAVS Inhibits HIV-1 Replication via Type I Interferon Secretion and Induction of HIV-1 Restriction Factors.
Authors: Gupta S, Termini J, Issac B, Guirado E, Stone G
PLoS ONE, 2016-02-05;11(2):e0148929.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
Plasmacytoid dendritic cells and myeloid cells differently contribute to BAFF over-expression during primary HIV infection.
Authors: Borhis G, Burelout C, Chaoul N, Smith N, Goujard C, Meyer L, Paul S, Saoudin H, Hosmalin A, Gilbert C, Herbeuval J, Richard Y
AIDS, 2016-01-28;30(0):365-376.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
Evidence for a pathophysiological role of keratinocyte-derived type III interferon (IFN-lambda) in cutaneous lupus erythematosus.
Authors: Zahn S, Rehkamper C, Kummerer BM, Ferring-Schmidt S, Bieber T, Tuting T, Wenzel J
J. Invest. Dermatol., 2010-08-19;131(1):133-40.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P
Neutralizing type I IFN antibodies trigger an IFN-like response in endothelial cells.
Authors: Moll HP, Freudenthaler H, Zommer A
J. Immunol., 2008-04-15;180(8):5250-6.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
Virally stimulated plasmacytoid dendritic cells produce chemokines and induce migration of T and NK cells.
Authors: Megjugorac NJ, Young HA, Amrute SB, Olshalsky SL, Fitzgerald-Bocarsly P
J. Leukoc. Biol., 2004-01-23;75(3):504-14.
Species: Human
Sample Types:
Applications: Flow Cytometry