Human IGFBP-1 Antibody

(5 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human IGFBP-1 in ELISAs and Western blots. In Western blots, detection of recombinant mouse IGFBP-1 is observed but no cross‑reactivity with recombinant human (rh) IGFBP‑2, rhIGFBP‑3, rhIGFBP‑4 or rhIGFBP-5 is observered.
  • Source
    Monoclonal Mouse IgG1 Clone # 33627
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    E. coli-derived recombinant human IGFBP-1
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
    • Human IGFBP-1 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Human IGFBP‑1 Antibody (Catalog # MAB675)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Human IGFBP‑1 Biotinylated Antibody (Catalog # BAF871)
  • ELISA Standard
     
    Recombinant Human IGFBP-1 Protein, CF (Catalog # 871-B1)
  • Neutralization
    Measured by its ability to neutralize IGFBP‑1 inhibition of IGF‑I-dependent proliferation in the MCF‑7 human breast cancer cell line. Karey, K.P. et al. (1988) Cancer Research 48:4083. The Neutralization Dose (ND50) is typically 10‑40 µg/mL in the presence of 5 µg/mL Recombinant Human IGFBP‑1 and 6 ng/mL Recombinant Human IGF‑I.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Recombinant Human and Mouse IGFBP‑1 by Western Blot. Western blot shows 25 ng of Recombinant Human IGFBP‑1 (Catalog # 871-B1), Recombinant Mouse IGFBP‑1 (Catalog # 1588-B1) and Recombinant Human IGFBP‑5 (Catalog # 875-B5). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human IGFBP‑1 Monoclonal Antibody (Catalog # MAB675) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IGFBP‑1 at approximately 26 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 3.
IGFBP‑1 Inhibition of IGF‑I-dependent Cell Proliferation and Neutralization by Human IGFBP‑1 Antibody. Recombinant Human IGFBP‑1 (Catalog # 871-B1) inhibits Recombinant Human IGF‑I (Catalog # 291-G1) induced proliferation in the MCF‑7 human breast cancer cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IGF‑I (6 ng/mL) activity elicited by Recombinant Human IGFBP‑1 (5 µg/mL) is neutralized (green line) by increasing concentrations of Human IGFBP‑1 Monoclonal Antibody (Catalog # MAB675). The ND50 is typically 10-40 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IGFBP-1

The superfamily of insulin-like growth factor (IGF) binding proteins include the six high-affinity IGF binding proteins (IGFBP) and at least four additional low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP). All IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule. IGFBPs modulate the biological activities of IGF proteins. Some IGFBPs may also have intrinsic bioactivity that is independent of their ability to bind IGF proteins. Post-transitional modifications of IGFBP, including glycosylation, phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins to IGF.

Human IGFBP-1 cDNA encodes a 259 amino acid (aa) residue precursor protein with a putative 25 aa residue signal peptide that is processed to generate the 234 aa residue mature protein. IGFBP-1 contains an integrin receptor recognition sequence (RGD sequence) but lacks potential N-linked glycosylation sites. IGFBP-1 is expressed in liver, decidua, kidneys and is the most abundant IGFBP in amniotic fluid. Serum levels of IGFBP-1 are lowest after meals. Hepatocyte production of IGFBP-1 is regulated at the transcriptional level due to the affects of insulin and corticosteriods. IGFBP-1 binds equally well to IGF-I and IGF-II, with phosphorylated forms of IGFBP-1 exhibiting higher binding affinities.

  • References:
    1. Jones, J.I. and D.R. Clemmons (1995) Endocrine Rev. 16:3.
    2. Kelley, K.M. et al. (1996) Int. J. Biochem. Cell Biol. 28:619.
  • Long Name:
    Insulin-like Growth Factor Binding Protein 1
  • Entrez Gene IDs:
    3484 (Human); 16006 (Mouse)
  • Alternate Names:
    alpha-pregnancy-associated endometrial globulin; amniotic fluid binding protein; binding protein-25; binding protein-26; binding protein-28; growth hormone independent-binding protein; hIGFBP-1; IBP1; IBP-1; IGF-binding protein 1; IGFBP1; IGFBP-1; IGF-BP25; insulin-like growth factor binding protein 1; insulin-like growth factor-binding protein 1; Placental protein 12; PP12AFBP
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Species
Applications
Sample Type
  1. Glioblastoma-derived Macrophage Colony-stimulating Factor (MCSF) Induces Microglial Release of Insulin-like Growth Factor-binding Protein 1 (IGFBP1) to Promote Angiogenesis.
    Authors: Nijaguna M, Patil V, Urbach S, Shwetha S, Sravani K, Hegde A, Chandramouli B, Arivazhagan A, Marin P, Santosh V, Somasundaram K
    J Biol Chem, 2015;290(38):23401-15.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  2. Evaluation of human first trimester decidual and telomerase-transformed endometrial stromal cells as model systems of in vitro decidualization.
    Authors: Saleh L, Otti GR, Fiala C, Pollheimer J, Knofler M
    Reprod. Biol. Endocrinol., 2011;9(0):155.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  3. Biological variation in fasting serum insulin-like growth factor binding protein-1 (IGFBP-1) among individuals with a varying glucose tolerance.
    Authors: Borai A, Livingstone C, Mehta S, Zarif H, Abdelaal F, Ferns G
    Clin. Biochem., 2009;0(0):.
    Species: Human
    Sample Type: Serum
    Application: ELISA Development
  4. Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
    Authors: Hu H, Kwun J, Aizenstein BD, Knechtle SJ
    Transplantation, 2009;87(12):1814-20.
    Species: Human
    Sample Type: Urine
    Application: Antibody Array Development
  5. Matrix metalloproteinase-7 degrades all insulin-like growth factor binding proteins and facilitates insulin-like growth factor bioavailability.
    Authors: Nakamura M, Miyamoto S, Maeda H, Ishii G, Hasebe T, Chiba T, Asaka M, Ochiai A
    Biochem. Biophys. Res. Commun., 2005;333(3):1011-6.
    Species: Human
    Sample Type: Recombinant Protein
    Application: WB
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Isotype Control

Ctrl MAB002 138  
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Mouse IgG1 Isotype Control

Ctrl MAB002R 1
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse IgG Antibody

WB AF007 4
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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