Human IGFBP-6 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human IGFBP-6 in direct ELISAs. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse (rm) IGFBP-6 is observed, less than 4% cross-reactivity with rmIGFBP-3 and recombinant human (rh) IGFBP-4 is observed, and no cross-reactivity with rhIGFBP‑1, -2, -3, rmIGFBP-1, or -2 is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 164428
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human IGFBP-6
    Ala25-Gly240
    Accession # P24592
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Neutralization
    Measured by its ability to neutralize IGFBP‑6 inhibition of IGF‑II-dependent proliferation in the MCF‑7 human breast cancer cell line. Karey, K. P. et al. (1988) Cancer Research 48:4083. The Neutralization Dose (ND50) is typically 5‑25 µg/mL in the presence of 0.8 µg/mL Recombinant Human IGFBP‑6 and 14 ng/mL Recombinant Human IGF‑II.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
IGFBP‑6 Inhibition of IGF‑II-dependent Cell Proliferation and Neutralization by Human IGFBP‑6 Antibody. Recombinant Human IGFBP‑6 (Catalog # 876-B6) inhibits Recombinant Human IGF‑II (Catalog # 292-G2) induced proliferation in the MCF‑7 human breast cancer cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IGF‑II (14 ng/mL) activity elicited by Recombinant Human IGFBP‑6 (0.8 µg/mL) is neutralized (green line) by increasing concen­trations of Mouse Anti-Human IGFBP‑6 Monoclonal Antibody (Catalog # MAB8762). The ND50 is typically 5-25 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IGFBP-6

The superfamily of insulin-like growth factor (IGF) binding proteins include the six high-affinity IGF binding proteins (IGFBP) and at least four additional low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP). All IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule. IGFBPs modulate the biological activities of IGF proteins. Some IGFBPs may also have intrinsic bioactivity that is independent of their ability to bind IGF proteins. Post-transitional modifications of IGFBP, including glycosylation, phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins to IGF. Human IGFBP-6 cDNA encodes a 240 amino acid (aa) precursor protein with a putative 24 aa signal peptide that is processed to generate the 216 aa mature protein that is O-glycosylated. IGFBP-6 is expressed in ovarian cells, prostatic cells, and fibroblasts. IGFBP-6 is found predominantly in CSF and serum. IGFBP-6 binds preferentially to IGF-II, exhibiting a 2-fold higher affinity for IGF-II than for IGF-I.

  • References:
    1. Jones, J.I. and D.R. Clemmons (1995) Endocrine Rev. 16:3.
    2. Kelley, K.M. et al. (1996) Int. J. Biochem. Cell Biol. 28:619.
  • Long Name:
    Insulin-like Growth Factor Binding Protein 6
  • Entrez Gene IDs:
    3489 (Human); 16012 (Mouse)
  • Alternate Names:
    IBP6; IBP-6; IGF binding protein 6; IGF-binding protein 6; IGFBP6; IGFBP-6; insulin-like growth factor binding protein 6; insulin-like growth factor-binding protein 6
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