Human IgG3 Antibody Summary
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human IgG3 by Western Blot. Western blot shows lysates of purified human IgG1 (negative control), purified human IgG2 (negative control), purified human IgG3, and human purified human IgG4 (negative control). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human IgG3 Monoclonal Antibody (Catalog # MAB10122) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IgG3 at approximately 240 kDa (as indicated). Human IgG (Catalog # MAB1101) is shown as a loading control.This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
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