Human IgG Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB11012-100
MAB11012-SP
Human IgG Antibody in Data
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Human IgG Antibody Summary

Species Reactivity
Human
Specificity
Detects human IgG in direct ELISAs. As a pan-Human IgG-specific antibody, sandwich immunoassay specificity is determined by the capture antibody.
Source
Recombinant Monoclonal Rabbit IgG Clone # 1268C
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Purified IgG from human serum
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
ELISA

This antibody functions as a Human IgG ELISA detection antibody when paired with Mouse Anti-Human IgG1 Monoclonal Antibody (Catalog # MAB9894), Rabbit Anti-Human IgG1 Monoclonal Antibody (Catalog # MAB9930), Mouse Anti-Human IgG2 Monoclonal Antibody (Catalog # MAB9794), Rabbit Anti-Human IgG2 Monoclonal Antibody (Catalog # MAB97941), Mouse Anti-Human IgG4 Monoclonal Antibody (Catalog # MAB9895), Rabbit Anti-Human IgG4 Monoclonal Antibody (Catalog # MAB98951), or Human IgG Monoclonal Antibody (Catalog # MAB11013).

This product is intended for assay development on various assay platforms requiring antibody pairs.

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Human IgG1ELISA Standard Curve. Recombinant Human IgG1protein was serially diluted 2-fold and captured by Mouse Anti-Human IgG1Monoclonal Antibody (Catalog # MAB9894) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rabbit Anti-Human IgG Monoclonal Antibody (Catalog # MAB11012) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Human IgG2ELISA Standard Curve. Recombinant Human IgG2protein was serially diluted 2-fold and captured by Mouse Anti-Human IgG2Monoclonal Antibody (Catalog # MAB9794) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rabbit Anti-Human IgG Monoclonal Antibody (Catalog # MAB11012) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Human IgG4ELISA Standard Curve. Recombinant Human IgG4protein was serially diluted 2-fold and captured by Mouse Anti-Human IgG4Monoclonal Antibody (Catalog # MAB9895) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rabbit Anti-Human IgG Monoclonal Antibody (Catalog # MAB11012) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Human IgG ELISA Standard Curve. Recombinant Human IgG protein was serially diluted 2-fold and captured by Rabbit Anti-Human IgG Monoclonal Antibody (Catalog # MAB11013) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rabbit Anti-Human IgG Monoclonal Antibody (Catalog #MAB11012) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG

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