Human IgG4 Antibody

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Detection of Human IgG4by Western Blot.
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Product Details
Citations (1)
Supplemental Products

Human IgG4 Antibody Summary

Species Reactivity
Detects human IgG4 in direct ELISAs.
Monoclonal Mouse IgG2A Clone # 985547
Protein A or G purified from hybridoma culture supernatant
Purified IgG4 from normal human plasma
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
2 µg/mL
See below

This antibody functions as an ELISA capture antibody when paired with Rabbit Anti-Human IgG Monoclonal Antibody (Catalog # MAB11012).

This product is intended for assay development on various assay platforms requiring antibody pairs.


Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human IgG<sub>4</sub>by Western Blot. View Larger

Detection of Human IgG4by Western Blot. Western blot shows lysates of purified human IgG1(negative control), purified human IgG2(negative control), purified human IgG3(negative control), and human purified human IgG4. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human IgG4Monoclonal Antibody (Catalog # MAB9895) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IgG1at approximately 240 kDa (as indicated). Human IgG (Catalog # MAB1101) is shown as a loading control.This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

Human IgG4ELISA Standard Curve. Recombinant Human IgG4protein was serially diluted 2-fold and captured by Mouse Anti-Human IgG4Monoclonal Antibody (Catalog # MAB9895) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rabbit Anti-Human IgG Monoclonal Antibody (Catalog # MAB11012) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IgG4

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G4
Alternate Names
Ig gamma-4 chain C region; IgG4; immunoglobulin heavy constant gamma 4 (G4m marker); MGC117419

Product Datasheets

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Citation for Human IgG4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Skin basal cell carcinomas assemble a pro-tumorigenic spatially organized and self-propagating Trem2+ myeloid niche
    Authors: Haensel, D;Daniel, B;Gaddam, S;Pan, C;Fabo, T;Bjelajac, J;Jussila, AR;Gonzalez, F;Li, NY;Chen, Y;Hou, J;Patel, T;Aasi, S;Satpathy, AT;Oro, AE;
    Nature communications
    Species:  Human
    Sample Types: Organoids
    Applications: Neutralization Control


  1. Does the Human IgG4 Antibody (Cat# MAB9895) cross-react with IgG1, IgG2 or IgG3?

    • This antibody was obtained by IgG4 immunization. It does not detect IgG1, IgG2 and IgG3 by western blot. Considering that the different isotype subclasses differ in the constant region (and hinges),  it likely recognizes an epitope in the Fc region.

View all Antibody FAQs

Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

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