Human IgG2 Antibody
Human IgG2 Antibody Summary
This antibody functions as an ELISA capture antibody when paired with Rabbit Anti-Human IgG Monoclonal Antibody (Catalog # MAB11012).
This product is intended for assay development on various assay platforms requiring antibody pairs.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of IgG2by Western Blot. Western blot shows purified human IgG1(negative control), purified human IgG2, purified human IgG3(negative control), and human purified human IgG4(negative control). PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human IgG2Monoclonal Antibody (Catalog # MAB97941) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for IgG2at approximately 240 kDa (as indicated). Human IgG (Catalog # MAB1101) is shown as a loading control.This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
Human IgG2ELISA Standard Curve. Recombinant Human IgG2protein was serially diluted 2-fold and captured by Rabbit Anti-Human IgG2Monoclonal Antibody (Catalog # MAB97941) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rabbit Anti-Human IgG Monoclonal Antibody (Catalog # MAB11012) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
Do the Human IgG2 antibodies, Catalog #s MAB9794 and MAB97941, bind to the hinge region of IgG2?
We have not mapped the epitope of these antibodes. We observe no cross-reactivity to other closely related IgG isoforms IgG1, IgG3 and IgG4 by non-reducing Western blot.
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