Key Product Details

Species Reactivity

Human

Applications

ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 985028
View all IgG2 Primary Antibodies »

Product Specifications

Immunogen

Human IgG2 protein

Specificity

Detects human IgG2 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human IgG2 Antibody

Human IgG2ELISA Standard Curve.

Human IgG2ELISA Standard Curve.

Recombinant Human IgG2protein was serially diluted 2-fold and captured by Mouse Anti-Human IgG2Monoclonal Antibody (Catalog # MAB9794) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rabbit Anti-Human IgG Monoclonal Antibody (Catalog # MAB11012) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Applications for Human IgG2 Antibody

Application
Recommended Usage

ELISA

This antibody functions as an ELISA capture antibody when paired with Rabbit Anti-Human IgG Monoclonal Antibody (Catalog # MAB11012).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IgG2

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G2

Alternate Names

Constant region of heavy chain of IgG2, immunoglobulin gamma 2 (Gm marker), immunoglobulin heavy constant gamma 2 (Gm marker)

Additional IgG2 Products

Product Documents for Human IgG2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human IgG2 Antibody

For research use only

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FAQs for Human IgG2 Antibody

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  • Q: Do the Human IgG2 antibodies, Catalog #s MAB9794 and MAB97941, bind to the hinge region of IgG2?

    A: We have not mapped the epitope of these antibodes. We observe no cross-reactivity to other closely related IgG isoforms IgG1, IgG3 and IgG4 by non-reducing Western blot. 

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