Human IL-1 RAcP/IL-1 R3 Antibody

R&D Systems | Catalog # AF676

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Flow Cytometry, CyTOF-ready

Cited:

Western Blot, ELISA Development (Capture)

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all IL-1 RAcP/IL-1 R3 Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human  IL‑1 RAcP/IL‑1 R3
Ser21-Glu359
Accession # Q9NPH3

Specificity

Detects human IL‑1 RAcP/IL‑1 R3 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant mouse IL-1 RAcP is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human IL-1 RAcP/IL-1 R3 Antibody

Detection of IL‑1 RAcP/IL‑1 R3 in PBMC Monocytes by Flow Cytometry

Human peripheral blood monocytes were stained with Goat Anti-Human IL‑1 RAcP/IL‑1 R3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF676, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins.

Applications for Human IL-1 RAcP/IL-1 R3 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: Human peripheral blood monocytes

Western Blot

0.1 µg/mL
Sample: Recombinant Human IL‑1 RAcP/IL‑1 R3 Fc Chimera (Catalog # 676-CP)

Reviewed Applications

Read 2 reviews rated 5 using AF676 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.<br/>*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-1 RAcP/IL-1 R3

IL-1 Receptor Accessory Protein (also IL-1 R3) is a ubiquitous, 70-90 kDa member of the interleukin-1 receptor family of proteins (1-5). It serves as a
non‑ligand‑binding accessory component of the receptors for IL-1 alpha, IL-1 beta, and IL-33 (6, 7). Together with IRAK4 and MyD88, it generates a functional signaling complex with IL‑1 RI; by itself, it generates a non-signaling, but high-affinity binding complex with IL-1 RII (8). In addition, it interacts with ST2 on mast cells and Th2 T cells to create a functional IL-33 receptor complex (7). Mature human IL-1 RAcP is a type I transmembrane glycoprotein that is 550 amino acids in length. It contains a 347 amino acid (aa) extracellular region (aa 21-367), a 21 aa transmembrane segment, and a 182 aa cytoplasmic domain (9). The extracellular region shows three C2‑type Ig-like domains, the most membrane proximal of which is suggested to be responsible for dimerization with IL-1 RI (10). There are three alternative splice forms reported for IL-1 RAcP. One is transmembrane, and shows a 239 aa substitution for the C-terminal 122 amino acids (11). The other two are soluble; one shows a six aa substitution for aa 351-570, while a second shows a 45 aa substitution for aa 302-579 (12, 13). The soluble receptor isoforms appear to be inhibitory to IL-1 signaling. When present with soluble IL-1 RII, soluble IL-1 RAcP increases the IL-1 binding affinity of IL-1 RII more than 100-fold, thus neutralizing the effects of IL-1 (14). The human and mouse IL-1 RAcP precursors are 89% aa identical; within the extracellular region, they share 86% aa identity.

References

  1. Subramaniam, S. et al. (2004) Dev. Comp. Immunol. 28:415.
  2. Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
  3. Dunne, A. and L.A.J. O'Neill (2003) Sci STKE. Feb 25;2003(171):re3.
  4. Huang, J. et al. (1997) Proc. Natl. Acad. Sci. USA 94:12829.
  5. Greenfeder, S. A. et al. (1995) J. Biol. Chem. 270:13757.
  6. Brikos, C. et al. (2007) Mol. Cell. Proteomics 6:1551.
  7. Chackerian, A.A. et al. (2007) J. Immunol. 179:2551.
  8. Lang, D. et al. (1998) J. Immunol. 161:6871.
  9. SwissProt. Accession # Q9NPH3.
  10. Yoon, D-Y. and C.A. Dinarello (1998) J. Immunol. 160:3170.
  11. Lu, H-L. et al. (2008) Mol. Immunol. 45:1374.
  12. Jensen, L.E. et al. (2000) J. Immunol. 164:5277.
  13. Jensen, L.E. and A.S. Whitehead (2003) Cell. Signal. 15:793.
  14. Smith, D.E. et al. (2003) Immunity 18:87.

Long Name

Interleukin 1 Receptor Accessory Protein

Alternate Names

IL-1 R3, IL-1RAcP, IL1RAcP, IL1RAP

Entrez Gene IDs

3556 (Human); 16180 (Mouse)

Gene Symbol

IL1RAP

UniProt

Q9NPH3

Additional IL-1 RAcP/IL-1 R3 Products

Product Documents for Human IL-1 RAcP/IL-1 R3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human IL-1 RAcP/IL-1 R3 Antibody

For research use only

Citations for Human IL-1 RAcP/IL-1 R3 Antibody

Customer Reviews for Human IL-1 RAcP/IL-1 R3 Antibody (2)

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  • Human IL-1 RAcP/IL-1 R3 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 05/17/2019
    Human IL-1 RAcP/IL-1 R3 Antibody AF676
  • Human IL-1 RAcP/IL-1 R3 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma and Cell culture supernatant
    Species: Human
    Verified Customer | Posted 11/09/2017
    AF676was used as both the capture and the detection antibody for the sandwich ELISA for sIL-1R3. The immunoassay standard was 676-CP. Assay had sensitivity of ~6pg/ml.
    Human IL-1 RAcP/IL-1 R3 Antibody AF676

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