Human IL-12 Antibody

Catalog # Availability Size / Price Qty
AB-219-NA
Cell Proliferation Induced by IL‑12 and Neutralization by Human IL‑12 Antibody.
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Product Details
Citations (2)
FAQs
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Human IL-12 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-12 in direct ELISAs and Western blots. In these formats, less than 2% cross-reactivity with recombinant mouse IL-12 is observed.
Source
Polyclonal Goat IgG
Purification
Protein A or G purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑12 heterodimer
Arg23-Ser219 of p35; Ile23-Ser328 of p40
Accession # P29459 (p35) & P29460 (p40)
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human IL‑12 (Catalog # 219-IL)
Neutralization
Measured by its ability to neutralize IL‑12-induced proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) [Yokota, T. et al. (1986) Proc. Natl. Acad. Sci. USA 83:5894]. The Neutralization Dose (ND50) is typically 1-2 µg/mL in the presence of 1 ng/mL Recombinant Human IL‑12.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Neutralization Cell Proliferation Induced by IL‑12 and Neutralization by Human IL‑12 Antibody. View Larger

Cell Proliferation Induced by IL‑12 and Neutralization by Human IL‑12 Antibody. Recombinant Human IL‑12 (Catalog # 219-IL) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑12 (1 ng/mL) is neutralized (green line) by increasing concentrations of Human IL‑12 Polyclonal Antibody (Catalog # AB-219-NA). The ND50 is typically 1-2 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 1 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-12

Interleukin 12, also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine originally identified in the medium of activated human B lymphoblastoid cell lines. The p40 subunit of IL-12 has been shown to have extensive amino acid sequence homology to the extracellular domain of the human IL-6 receptor while the p35 subunit shows distant but significant sequence similarity to IL-6, G-CSF, and chicken MGF. These observations have led to the suggestion that IL-12 might have evolved from a cytokine/soluble receptor complex. Human and murine IL-12 share 70% and 60% amino acid sequence homology in their p40 and p35 subunits, respectively. IL-12 apparently shows species specificity with human IL-12 reportedly showing minimal activity in the murine system.

IL-12 is produced by macrophages and B lymphocytes and has been shown to have multiple effects on T cells and natural killer (NK) cells. These effects include inducing production of IFN-gamma and TNF by resting and activated T and NK cells, synergizing with other IFN-gamma  inducers at both the transcriptional and post-transcriptional levels. This interaction induces IFN-gamma  gene expression, enhancing the cytotoxic activity of resting NK and T cells, inducing and synergizing with IL-2 in the generation of lymphokine-activated killer (LAK) cells, acting as a co-mitogen to stimulate proliferation of resting T cells, and inducing proliferation of activated T and NK cells. Current evidence indicates that IL-12, produced by macrophages in response to infectious agents, is a central mediator of the cell-mediated immune response by its actions on the development, proliferation, and activities of TH1 cells. In its role as the initiator of cell-mediated immunity, it has been suggested that IL-12 has therapeutic potential as a stimulator of cell-mediated immune responses to microbial pathogens, metastatic cancers, and viral infections such as AIDS.

Long Name
Interleukin 12
Entrez Gene IDs
3592 (Human); 16159 (Mouse); 84405 (Rat); 403977 (Canine); 493741 (Feline)
Alternate Names
CLMF p35; CLMF1; Cytotoxic lymphocyte maturation factor 35 kDa subunit; IL-12 subunit p35; IL12; IL-12; IL-12, subunit p35; IL12A; interleukin 12, p35; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35); interleukin-12 alpha chain; interleukin-12 subunit alpha; natural killer cell stimulatory factor 1, 35 kD subunit; NF cell stimulatory factor chain 1; NK cell stimulatory factor chain 1; NKSF1; p35

Product Datasheets

Citations for Human IL-12 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. A Novel Bromodomain Inhibitor Reverses HIV-1 Latency through Specific Binding with BRD4 to Promote Tat and P-TEFb Association
    Authors: H Huang, S Liu, M Jean, S Simpson, H Huang, M Merkley, T Hayashi, W Kong, I Rodríguez-, X Zhang, HO Yosief, H Miao, J Que, JJ Kobie, J Bradner, NG Santoso, W Zhang, J Zhu
    Front Microbiol, 2017;8(0):1035.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  2. IL-33 induces IL-9 production in human CD4+ T cells and basophils.
    Authors: Blom L, Poulsen BC, Jensen BM, Hansen A, Poulsen LK
    PLoS ONE, 2011;6(7):e21695.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut

FAQs

  1. Does the human IL-12 polyclonal antibody (Catalog # AB-219-NA) recognize IL-12 p70?

    • In Western blots, this antibody recognizes the p70 heterodimer as well as the p40 and p35 subunits.

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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