Human IL-13 R alpha 2 Antibody

(15 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human IL-13 R alpha 2 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse IL-13 R alpha 2 is observed and less than 1% cross-reactivity with recombinant human IL-13 R alpha 1 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human IL‑13 R alpha 2
    Cys22-Leu342
    Accession # Q14627
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.2 µg/mL
    See below
  • Simple Western
    10 µg/mL
    See below
  • Flow Cytometry
    2.5 µg/106 cells
    A375 human melanoma cell line
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • Blockade of Receptor-ligand Interaction
    In a functional ELISA, 2-6 µg/mL of this antibody will block 50% of the binding of 100 ng/mL of Recombinant Human IL-13 (Catalog # 213‑ILB) to immobilized Recombinant Human IL-13 R alpha 2 Fc Chimera (Catalog # 614-INS) coated at 4 µg/mL (100 µL/well). At 40 μg/mL, this antibody will block >90% of the binding.
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples

Detection of Human IL‑13 R alpha 2 by Western Blot. Western blot shows lysates of human placenta tissue and human testis tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human IL‑13 R alpha 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF146) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL‑13 R alpha 2 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

IL-13 R alpha 2 in Human Prostate Cancer Tissue. IL‑13 R alpha 2 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Goat Anti-Human IL‑13 R alpha 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF146) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to glandular epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human IL‑13 R alpha 2 by Simple WesternTM. Simple Western lane view shows lysates of human placenta tissue and human testis tissue, loaded at 0.2 mg/mL. A specific band was detected for IL‑13 R alpha 2 at approximately 40 kDa (as indicated) using 10 µg/mL of Goat Anti-Human IL‑13 R alpha 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF146) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.        

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-13 R alpha 2

Two type1 membrane proteins belonging to the hemopoietin receptor family have been cloned and shown to bind IL-13 with differing affinities. The lower affinity IL-13 binding protein, previously designated IL-13 R alpha, IL-13 R alpha l or NR4, is now referred to as IL-13 R alpha 1. The high affinity IL-13 binding protein, previously also designated IL-13 R or IL-13 R alpha l, is now referred to as IL-13 R alpha 2. Human IL-13 R alpha 2 was originally cloned from the Caki-1 human renal carcinoma cell line. The IL-13 R alpha 2 cDNA encodes a 380 amino acid (aa) residue precursor protein with a putative 26 aa residue signal peptide, a 317 residue extracellular domain, a 20 aa residue transmembrane region and a 17 aa residue cytoplasmic tail. Human and mouse IL‑13 R alpha 2 share 59% aa sequence identity. The extracellular domain of IL-13 R alpha 2 is also closely related to that of IL-13 R alpha 1. However, the 17 aa residue cytoplasmic domain of IL-13 R alpha 2 is much shorter than that of IL-13 R alpha 1, suggesting that the two receptors are functionally distinct. IL-13 R alpha 1 has been shown to combine with the IL-4 R to form a high-affinity receptor complex capable of transducing an IL‑13‑dependent proliferative signal. The role of IL-13 R alpha 2 in IL-13 signaling remains to be elucidated. The amino-terminal 27 aa residues of the human and mouse IL‑13 R alpha 2 are nearly identical to that of a soluble mouse IL-13 binding protein purified from mouse serum and urine.

  • References:
    1. Caput, D. et al. (1996) J. Biol. Chem. 271:16921.
    2. Donaldson, D.D. et al. (1998) J. Immunol. 161:2317.
    3. Aman, M.J. et al. (1996) J. Biol. Chem. 271:29265.
    4. Hilton, D.J. et al. (1996) Proc. Natl. Acad. Sci. USA 93:497.
    5. Zhang, J.G. et al. (1997) J. Biol. Chem. 272:9474.
    6.  
  • Long Name:
    Interleukin 13 Receptor alpha 2
  • Entrez Gene IDs:
    3598 (Human); 16165 (Mouse)
  • Alternate Names:
    cancer/testis antigen 19; CD213a2 antigen; CD213a2; CT19; IL-13 R alpha 2; IL-13 receptor subunit alpha-2; IL13BP; IL13R alpha 2; IL-13R subunit alpha-2; IL13R; IL-13R; IL13RA2; IL-13Ra2; IL-13R-alpha-2; interleukin 13 binding protein; interleukin 13 receptor alpha 2 chain; interleukin 13 receptor, alpha 2; interleukin-13 receptor subunit alpha-2; Interleukin-13-binding protein
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. IL-4 primes the dynamics of breast cancer progression via DUSP4 inhibition
    Authors: M Gaggianesi, A Turdo, A Chinnici, E Lipari, T Apuzzo, A Benfante, I Sperduti, S Di Franco, S Meraviglia, E Lo Presti, F Dieli, V Caputo, G Militello, S Vieni, G Stassi, M Todaro
    Cancer Res., 2017;0(0):.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. IL-13R?2 uses TMEM219 in chitinase 3-like-1-induced signalling and effector responses
    Nat Commun, 2016;7(0):12752.
    Species: Human
    Sample Type: Cell Lysates
    Application: IP
  3. Role of IL13RA2 in Sunitinib Resistance in Clear Cell Renal Cell Carcinoma.
    Authors: Shibasaki N, Yamasaki T, Kanno T, Arakaki R, Sakamoto H, Utsunomiya N, Inoue T, Tsuruyama T, Nakamura E, Ogawa O, Kamba T
    PLoS ONE, 2015;10(6):e0130980.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC - Paraffin embedded
  4. IL-13Ralpha2 mediates PNR-induced migration and metastasis in ERalpha-negative breast cancer.
    Authors: Zhao Z, Wang L, Xu W
    Oncogene, 2015;34(12):1596-607.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  5. Expression of surfactant protein D in airways of asthmatics and interleukin-13 modulation of surfactant protein D in human models of airway epithelium.
    Authors: Xu J, Singhera G, Dorscheid D
    Respir Res, 2015;16(0):26.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  6. Absence of a role for interleukin-13 in inflammatory bowel disease.
    Authors: Biancheri P, Di Sabatino A, Ammoscato F, Facciotti F, Caprioli F, Curciarello R, Hoque S, Ghanbari A, Joe-Njoku I, Giuffrida P, Rovedatti L, Geginat J, Corazza G, MacDonald T
    Eur J Immunol, 2014;44(2):370-85.
  7. Cytokine induction of VCAM-1 but not IL13Ralpha2 on glioma cells: a tale of two antibodies.
    Authors: Mahadev V, Starr R, Wright S, Martinez C, Jensen M, Barish M, Forman S, Brown C
    PLoS ONE, 2014;9(5):e95123.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  8. Matrix metalloproteinase 8 contributes to solubilization of IL-13 receptor alpha2 in vivo.
    Authors: Chen W, Tabata Y, Gibson AM, Daines MO, Warrier MR, Wills-Karp M, Hershey GK
    J. Allergy Clin. Immunol., 2008;122(3):625-32.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  9. Interleukin-13 displaying retargeted oncolytic measles virus strains have significant activity against gliomas with improved specificity.
    Authors: Allen C, Paraskevakou G, Iankov I, Giannini C, Schroeder M, Sarkaria J, Schroeder M, Puri RK, Russell SJ, Galanis E
    Mol. Ther., 2008;16(9):1556-64.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  10. Development of an in vitro potency bioassay for therapeutic IL-13 antagonists: the A-549 cell bioassay.
    Authors: Miller R, Sadhukhan R, Wu C
    J. Immunol. Methods, 2008;334(1):134-41.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  11. Effect of IL-13 receptor alpha2 levels on the biological activity of IL-13 variant R110Q.
    Authors: Andrews AL, Bucchieri F, Arima K, Izuhara K, Holgate ST, Davies DE, Holloway JW
    J. Allergy Clin. Immunol., 2007;120(1):91-7.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  12. IL-13 receptor alpha 2: a regulator of IL-13 and IL-4 signal transduction in primary human fibroblasts.
    Authors: Andrews AL, Nasir T, Bucchieri F, Holloway JW, Holgate ST, Davies DE
    J. Allergy Clin. Immunol., 2006;118(4):858-65.
    Species: Human
    Sample Type: Cell Lysates
    Application: IP
  13. Level of expression of IL-13R alpha 2 impacts receptor distribution and IL-13 signaling.
    Authors: Daines MO, Tabata Y, Walker BA, Chen W, Warrier MR, Basu S, Hershey GK
    J. Immunol., 2006;176(12):7495-501.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  14. Protection of human conjunctival fibroblasts from NO-induced apoptosis by interleukin-4 or interleukin-13.
    Authors: Fujitsu Y, Fukuda K, Kimura K, Seki K, Kumagai N, Nishida T
    Invest. Ophthalmol. Vis. Sci., 2005;46(3):797-802.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  15. Soluble interleukin-13Ralpha2 decoy receptor inhibits Hodgkin's lymphoma growth in vitro and in vivo.
    Authors: Trieu Y, Wen XY, Skinnider BF, Bray MR, Li Z, Claudio JO, Masih-Khan E, Zhu YX, Trudel S, McCart JA, Mak TW, Stewart AK
    Cancer Res., 2004;64(9):3271-5.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: IP
Expand to show all 15 Citations
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