Human IL-13 R alpha 2 Antibody

• Species Reactivity
  Human
• Specificity
  Detects human IL-13 R alpha 2 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse IL-13 R alpha 2 is observed and less than 1% cross-reactivity with recombinant human IL-13 R alpha 1 is observed.
• Source
  Polyclonal Goat IgG
• Purification
  Antigen Affinity-purified
• Immunogen
  Mouse myeloma cell line NS0-derived recombinant human IL‑13 R alpha 2
  Cys22-Leu342
  Accession # Q14627
• Formulation
  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Endotoxin Level
  <0.10 EU per 1 μg of the antibody by the LAL method.
• Label
  Unconjugated

• Western Blot
  0.2 µg/mL
  See below
• Simple Western
  10 µg/mL
  See below
• Flow Cytometry
  2.5 µg/10⁶ cells
  A375 human melanoma cell line
• Immunohistochemistry
  5-15 µg/mL
  See below
• Blockade of Receptor-ligand Interaction
  In a functional ELISA, 2-6 µg/mL of this antibody will block 50% of the binding of 100 ng/mL of Recombinant Human IL-13 (Catalog # 213‑ILB) to immobilized Recombinant Human IL-13 R alpha 2 Fc Chimera (Catalog # 614-INS) coated at 4 µg/mL (100 µL/well). At 40 μg/mL, this antibody will block >90% of the binding.
• CyTOF-ready
  Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Western Blot click image to view larger	Detection of Human IL‑13 R alpha 2 by Western Blot. Western blot shows lysates of human placenta tissue and human testis tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human IL‑13 R alpha 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF146) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL‑13 R alpha 2 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry click image to view larger	IL-13 R alpha 2 in Human Prostate Cancer Tissue. IL‑13 R alpha 2 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Goat Anti-Human IL‑13 R alpha 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF146) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to glandular epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Simple Western click image to view larger	Detection of Human IL‑13 R alpha 2 by Simple WesternTM. Simple Western lane view shows lysates of human placenta tissue and human testis tissue, loaded at 0.2 mg/mL. A specific band was detected for IL‑13 R alpha 2 at approximately 40 kDa (as indicated) using 10 µg/mL of Goat Anti-Human IL‑13 R alpha 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF146) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reconstitution
  Reconstitute at 0.2 mg/mL in sterile PBS.

  Reconstitution Buffer Available
• Shipping
  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
• Stability & Storage
  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

• References:
  1. Caput, D. et al. (1996) J. Biol. Chem. 271:16921.
  2. Donaldson, D.D. et al. (1998) J. Immunol. 161:2317.
  3. Aman, M.J. et al. (1996) J. Biol. Chem. 271:29265.
  4. Hilton, D.J. et al. (1996) Proc. Natl. Acad. Sci. USA 93:497.
  5. Zhang, J.G. et al. (1997) J. Biol. Chem. 272:9474.
   

• Long Name:
  Interleukin 13 Receptor alpha 2

• Entrez Gene IDs:
  3598 (Human); 16165 (Mouse)

• Alternate Names:
  cancer/testis antigen 19; CD213a2 antigen; CD213a2; CT19; IL-13 R alpha 2; IL-13 receptor subunit alpha-2; IL13BP; IL13R alpha 2; IL-13R subunit alpha-2; IL13R; IL-13R; IL13RA2; IL-13Ra2; IL-13R-alpha-2; interleukin 13 binding protein; interleukin 13 receptor alpha 2 chain; interleukin 13 receptor, alpha 2; interleukin-13 receptor subunit alpha-2; Interleukin-13-binding protein

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