Human IL-13 R alpha 2 Antibody Summary
Cys22-Leu342
Accession # Q14627
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples

Detection of Human IL‑13 R alpha 2 by Western Blot. Western blot shows lysates of human placenta tissue and human testis tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human IL-13 Ra2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF146) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL-13 Ra2 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

IL-13 R alpha 2 in Human Prostate Cancer Tissue. IL-13 Ra2 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Goat Anti-Human IL-13 Ra2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF146) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to glandular epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human IL‑13 R alpha 2 by Simple WesternTM. Simple Western lane view shows lysates of human placenta tissue and human testis tissue, loaded at 0.2 mg/mL. A specific band was detected for IL-13 Ra2 at approximately 59 kDa (as indicated) using 10 µg/mL of Goat Anti-Human IL-13 Ra2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF146) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-13 R alpha 2
Two type1 membrane proteins belonging to the hemopoietin receptor family have been cloned and shown to bind IL-13 with differing affinities. The lower affinity IL-13 binding protein, previously designated IL-13 R alpha, IL-13 R alpha l or NR4, is now referred to as IL-13 R alpha 1. The high affinity IL-13 binding protein, previously also designated IL-13 R or IL-13 R alpha l, is now referred to as IL-13 R alpha 2. Human IL-13 R alpha 2 was originally cloned from the Caki-1 human renal carcinoma cell line. The IL-13 R alpha 2 cDNA encodes a 380 amino acid (aa) residue precursor protein with a putative 26 aa residue signal peptide, a 317 residue extracellular domain, a 20 aa residue transmembrane region and a 17 aa residue cytoplasmic tail. Human and mouse IL‑13 R alpha 2 share 59% aa sequence identity. The extracellular domain of IL-13 R alpha 2 is also closely related to that of IL-13 R alpha 1. However, the 17 aa residue cytoplasmic domain of IL-13 R alpha 2 is much shorter than that of IL-13 R alpha 1, suggesting that the two receptors are functionally distinct. IL-13 R alpha 1 has been shown to combine with the IL-4 R to form a high-affinity receptor complex capable of transducing an IL‑13‑dependent proliferative signal. The role of IL-13 R alpha 2 in IL-13 signaling remains to be elucidated. The amino-terminal 27 aa residues of the human and mouse IL‑13 R alpha 2 are nearly identical to that of a soluble mouse IL-13 binding protein purified from mouse serum and urine.
- Caput, D. et al. (1996) J. Biol. Chem. 271:16921.
- Donaldson, D.D. et al. (1998) J. Immunol. 161:2317.
- Aman, M.J. et al. (1996) J. Biol. Chem. 271:29265.
- Hilton, D.J. et al. (1996) Proc. Natl. Acad. Sci. USA 93:497.
- Zhang, J.G. et al. (1997) J. Biol. Chem. 272:9474.
Product Datasheets
Citations for Human IL-13 R alpha 2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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The IL-4/IL-13 signaling axis promotes prostatic fibrosis
Authors: Q D'Arcy, M Gharaee-Ke, A Zhilin-Rot, JA Macoska
PLoS ONE, 2022;17(10):e0275064.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
IL-13 receptors as possible therapeutic targets in diffuse intrinsic pontine glioma
Authors: NE Berlow, MN Svalina, MJ Quist, TP Settelmeye, V Zherebitsk, M Kogiso, L Qi, Y Du, CE Hawkins, E Hulleman, XN Li, SH Gultekin, C Keller
PLoS ONE, 2018;13(4):e0193565.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Optimization of IL13R?2-Targeted Chimeric Antigen Receptor T Cells for Improved Anti-tumor Efficacy against Glioblastoma
Authors: CE Brown, B Aguilar, R Starr, X Yang, WC Chang, L Weng, B Chang, A Sarkissian, A Brito, JF Sanchez, JR Ostberg, M D'Apuzzo, B Badie, ME Barish, SJ Forman
Mol. Ther., 2018;26(1):31-44.
Species: Human
Sample Types: Xenograft
Applications: Flow Cytometry -
Identification of a novel role of IL-13R?2 in human Glioblastoma multiforme: interleukin-13 mediates signal transduction through AP-1 pathway
Authors: R Bhardwaj, A Suzuki, P Leland, BH Joshi, RK Puri
J Transl Med, 2018;16(1):369.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
IL-4 primes the dynamics of breast cancer progression via DUSP4 inhibition
Authors: M Gaggianesi, A Turdo, A Chinnici, E Lipari, T Apuzzo, A Benfante, I Sperduti, S Di Franco, S Meraviglia, E Lo Presti, F Dieli, V Caputo, G Militello, S Vieni, G Stassi, M Todaro
Cancer Res., 2017;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Cigarette smoke extracts induce overexpression of the proto-oncogenic gene interleukin-13 receptor ?2 through activation of the PKA-CREB signaling pathway to trigger malignant transformation of lung vascular endothelial cells and angiogenesis
Authors: Mei Meng
Cell. Signal, 2016;0(0):.
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IL-13R?2 uses TMEM219 in chitinase 3-like-1-induced signalling and effector responses
Nat Commun, 2016;7(0):12752.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation -
Expression of surfactant protein D in airways of asthmatics and interleukin-13 modulation of surfactant protein D in human models of airway epithelium.
Authors: Xu J, Singhera G, Dorscheid D
Respir Res, 2015;16(0):26.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
IL-13Ralpha2 mediates PNR-induced migration and metastasis in ERalpha-negative breast cancer.
Authors: Zhao Z, Wang L, Xu W
Oncogene, 2015;34(12):1596-607.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Role of IL13RA2 in Sunitinib Resistance in Clear Cell Renal Cell Carcinoma.
Authors: Shibasaki N, Yamasaki T, Kanno T, Arakaki R, Sakamoto H, Utsunomiya N, Inoue T, Tsuruyama T, Nakamura E, Ogawa O, Kamba T
PLoS ONE, 2015;10(6):e0130980.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Cytokine induction of VCAM-1 but not IL13Ralpha2 on glioma cells: a tale of two antibodies.
Authors: Mahadev V, Starr R, Wright S, Martinez C, Jensen M, Barish M, Forman S, Brown C
PLoS ONE, 2014;9(5):e95123.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Absence of a role for interleukin-13 in inflammatory bowel disease.
Authors: Biancheri P, Di Sabatino A, Ammoscato F, Facciotti F, Caprioli F, Curciarello R, Hoque S, Ghanbari A, Joe-Njoku I, Giuffrida P, Rovedatti L, Geginat J, Corazza G, MacDonald T
Eur J Immunol, 2014;44(2):370-85.
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Interleukin-13 displaying retargeted oncolytic measles virus strains have significant activity against gliomas with improved specificity.
Authors: Allen C, Paraskevakou G, Iankov I, Giannini C, Schroeder M, Sarkaria J, Schroeder M, Puri RK, Russell SJ, Galanis E
Mol. Ther., 2008;16(9):1556-64.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Development of an in vitro potency bioassay for therapeutic IL-13 antagonists: the A-549 cell bioassay.
Authors: Miller R, Sadhukhan R, Wu C
J. Immunol. Methods, 2008;334(1):134-41.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Matrix metalloproteinase 8 contributes to solubilization of IL-13 receptor alpha2 in vivo.
Authors: Chen W, Tabata Y, Gibson AM, Daines MO, Warrier MR, Wills-Karp M, Hershey GK
J. Allergy Clin. Immunol., 2008;122(3):625-32.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Effect of IL-13 receptor alpha2 levels on the biological activity of IL-13 variant R110Q.
Authors: Andrews AL, Bucchieri F, Arima K, Izuhara K, Holgate ST, Davies DE, Holloway JW
J. Allergy Clin. Immunol., 2007;120(1):91-7.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
IL-13 receptor alpha 2: a regulator of IL-13 and IL-4 signal transduction in primary human fibroblasts.
Authors: Andrews AL, Nasir T, Bucchieri F, Holloway JW, Holgate ST, Davies DE
J. Allergy Clin. Immunol., 2006;118(4):858-65.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation -
Level of expression of IL-13R alpha 2 impacts receptor distribution and IL-13 signaling.
Authors: Daines MO, Tabata Y, Walker BA, Chen W, Warrier MR, Basu S, Hershey GK
J. Immunol., 2006;176(12):7495-501.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Protection of human conjunctival fibroblasts from NO-induced apoptosis by interleukin-4 or interleukin-13.
Authors: Fujitsu Y, Fukuda K, Kimura K, Seki K, Kumagai N, Nishida T
Invest. Ophthalmol. Vis. Sci., 2005;46(3):797-802.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Soluble interleukin-13Ralpha2 decoy receptor inhibits Hodgkin's lymphoma growth in vitro and in vivo.
Authors: Trieu Y, Wen XY, Skinnider BF, Bray MR, Li Z, Claudio JO, Masih-Khan E, Zhu YX, Trudel S, McCart JA, Mak TW, Stewart AK
Cancer Res., 2004;64(9):3271-5.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Immunoprecipitation -
Tandem CAR T cells targeting HER2 and IL13Ralpha2 mitigate tumor antigen escape.
Authors: Hegde M, Mukherjee M, Grada Z, Pignata A, Landi D, Navai S, Wakefield A, Fousek K, Bielamowicz K, Chow K, Brawley V, Byrd T, Krebs S, Gottschalk S, Wels W, Baker M, Dotti G, Mamonkin M, Brenner M, Orange J, Ahmed N
J Clin Invest, 0;126(8):3036-52.
Species: Human
Sample Types: Whole Cells
Applications: CAR-T (Flow Cytometry) -
Combinational targeting offsets antigen escape and enhances effector functions of adoptively transferred T cells in glioblastoma.
Authors: Hegde M, Corder A, Chow K, Mukherjee M, Ashoori A, Kew Y, Zhang Y, Baskin D, Merchant F, Brawley V, Byrd T, Krebs S, Wu M, Liu H, Heslop H, Gottschalk S, Yvon E, Ahmed N
Mol Ther, 0;21(11):2087-101.
Species: Human
Sample Types: Whole Tissue
Applications: CAR-T (IHC-P) -
Trivalent CAR T cells overcome interpatient antigenic variability in glioblastoma.
Authors: Bielamowicz K, Fousek K, Byrd T, Samaha H, Mukherjee M, Aware N, Wu M, Orange J, Sumazin P, Man T, Joseph S, Hegde M, Ahmed N
Neuro Oncol, 0;20(4):506-518.
Species: Xenograft
Sample Types: Whole Tissue
Applications: CAR-T (IHC-F) -
Bioactivity and Safety of IL13Ralpha2-Redirected Chimeric Antigen Receptor CD8+ T Cells in Patients with Recurrent Glioblastoma.
Authors: Brown C, Badie B, Barish M, Weng L, Ostberg J, Chang W, Naranjo A, Starr R, Wagner J, Wright C, Zhai Y, Bading J, Ressler J, Portnow J, D'Apuzzo M, Forman S, Jensen M
Clin Cancer Res, 0;21(18):4062-72.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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