|Cell Proliferation Induced by IL‑19 and Neutralization by Human IL‑19 Antibody. Recombinant Human IL‑19 (Catalog # 1035-IL) stimulates proliferation in the BaF3 mouse pro‑B cell line co-transfected with human IL‑20 R alpha and IL‑20 R beta in a dose‑dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑19 (3 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‑19 Monoclonal Antibody (Catalog # MAB10351). The ND50 is typically 0.1‑0.4 µg/mL.|
Human Interleukin 19 (IL-19) is a member of the IL-10 family of related cytokines. Its gene contains two alternate translation initiation sites, generating precursors of 215 amino acids (aa) and 177 aa, respectively. Both isoforms are processed to 17 kDa, 153 aa mature molecules. IL-19 contains seven helices and is secreted as a 35 kDa monomer. There are two potential N-linked glycosylation sites, and it is likely that the molecule is glycosylated. Mature human IL-19 shares 69% aa sequence identity with mouse IL-19. Although mouse IL-19 is active on human cells, human IL-19 is not active on mouse cells. IL-19 expression is limited to activated keratinocytes and monocytes. IL-19 binds a receptor complex consisting of the IL-20 receptor alpha (IL-20 R alpha, also known as IL-20 R1) and the IL-20 receptor beta (IL-20 R beta or IL-20 R2). This receptor complex is also shared by IL-20 and IL-24. Functionally, IL-19 induces IL-6 and TNF-alpha production by monocytes, and drives T-helper cell differentiation towards a Th2 response (1‑5).
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