Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human IL-19 Leu25-Ala177 Accession # AAN40906
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
Human LPS-treated peripheral blood mononuclear cells fixed with paraformaldehyde and permeabilized with saponin
Measured by its ability to neutralize IL‑19-induced proliferation in the BaF3 mouse pro‑B cell line co-transfected with human IL‑20 R alpha and IL‑20 R beta. The Neutralization Dose (ND50) is typically 0.2-0.5 µg/mL in the presence of 3 ng/mL Recombinant Human IL‑19.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Cell Proliferation Induced by IL‑19 and Neutralization by Human IL‑19 Antibody. Recombinant Human IL‑19 (Catalog # 1035-IL) stimulates proliferation in the BaF3 mouse pro‑B cell line co-transfected with human IL‑20 R alpha and IL‑20 R beta in a dose‑dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑19 (3 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‑19 Monoclonal Antibody (Catalog # MAB1035). The ND50 is typically 0.2‑0.5 µg/mL.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Human Interleukin 19 (IL-19) is a member of the IL-10 family of related cytokines. Its gene contains two alternate translation initiation sites, generating precursors of 215 amino acids (aa) and 177 aa, respectively. Both isoforms are processed to 17 kDa, 153 aa mature molecules. IL-19 contains seven helices and is secreted as a 35 kDa monomer. There are two potential N-linked glycosylation sites, and it is likely that the molecule is glycosylated. Mature human IL-19 shares 69% aa sequence identity with the mature mouse homologue. Although mouse IL-19 is active on human cells, human IL-19 is not active on mouse cells. IL-19 expression is limited to activated keratinocytes and monocytes. IL-19 binds a receptor complex consisting of the IL-20 receptor alpha (IL-20 R alpha, also known as IL-20 R1) and the IL-20 receptor beta (IL-20 R beta or IL-20 R2). This receptor complex is also shared by IL-20 and IL-24. Functionally, IL-19 induces IL-6 and TNF-alpha production by monocytes, and drives T-helper cell differentiation towards a Th2 response (1‑5).
Gallagher, G. et al. (2000) Genes Immun. 1:442.
Gallagher, G. et al. (2004) Int. Immunopharmacol. 4:615.
Laio, Y-C. et al. (2002) J. Immunol. 169:4288.
Romer, J. et al. (2003) J. Invest. Dermatol. 121:1306.
Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.