hh Human IL-20 Antibody AF1102: R&D Systems

Human IL-20 Antibody

(3 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human IL‑20 in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant human (rh) IL‑19 is observed, approximately 15% cross-reactivity with recombinant mouse IL‑20 is observed, and less than 1% cross-reactivity with rhIL‑10 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human IL‑20
    Leu25-Glu176
    Accession # AAF36679
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human IL‑20 (Catalog # 1102-IL)
  • Neutralization
    Measured by its ability to neutralize IL‑20-induced proliferation in the BaF3 mouse pro‑B cell line co-transfected with human IL‑20 R alpha and IL‑20 R beta. The Neutralization Dose (ND50) is typically 0.1-0.4 µg/mL in the presence of 2 ng/mL Recombinant Human IL‑20.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cell Proliferation Induced by IL‑20 and Neutralization by Human IL‑20 Antibody. Recombinant Human IL‑20 (Catalog # 1102-IL) stimulates proliferation in the BaF3 mouse pro‑B cell line co-transfected with human IL‑20 R alpha and IL‑20 R beta in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑20 (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑20 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1102). The ND50 is typically 0.1-0.4 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-20

Human Interleukin 20 (IL-20) was identified by searching sequence databases for translated sequences containing a signal sequence and amphipathic helices found in helical cytokines. Human IL-20 is synthesized as a 176 amino acid (aa) precursor with a 24 aa signal sequence and a 152 aa mature segment. There are no N-linked glycosylation sites and it is doubtful that the native molecule is glycosylated. Although IL-20 is a distant member of the IL-10 family, it functions as a monomer. IL‑20 shares less than 40% aa sequence identity with other IL-10 family members. Mouse and human IL‑20 share 77% aa sequence identity in their mature segments. Human IL‑20 is active on mouse cells. IL-20 production has been found in skin and trachea. In particular, activated keratinocytes and, possibly, monocytes are reported to express IL-20. There are two heterodimeric receptor complexes for IL-20. The first is composed of IL-20 R alpha and IL-20 R beta. The second is composed of IL‑22 R and IL-20 R beta. Whereas the IL-22 R/IL-20 R beta complex is shared with IL-24/mda-7, the IL-20 R alpha /IL-20 R beta  complex is shared with both IL-19 and IL-24. Little is known about the function of IL-20. It is reported to induce the proliferation of multipotential hematopoietic progenitor cells, direct the differentiation and expansion of keratinocytes, and promote the release of proinflammatory mediators in keratinocytes and other IL-20 receptor expressing cells (1-6).

  • References:
    1. Blumberg, H. et al. (2001) Cell 104:9.
    2. Liu, L. et al. (2003) Blood 102:3206. 
    3. Rich, B.E. and T.S. Kupper (2001) Curr. Biol. 11:R531.
    4. Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929. 
    5. Dumoutier, L. (2001) J. Immunol. 167:3545. 
    6. Romer, J. (2003) J. Invest. Dermatol. 121:1306.
  • Long Name:
    Interleukin 20
  • Entrez Gene IDs:
    50604 (Human); 58181 (Mouse)
  • Alternate Names:
    Cytokine Zcyto10; IL10D; IL20; IL-20; IL-20ZCYTO10four alpha helix cytokine; interleukin 20; interleukin-20; MGC96907
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. IL-20 Signaling in Activated Human Neutrophils Inhibits Neutrophil Migration and Function
    Authors: P Gough, S Ganesan, SK Datta
    J. Immunol., 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Functional Assay
  2. Induction of phenotype modifying cytokines by FERMT1 mutations.
    Authors: Heinemann A, He Y, Zimina E, Boerries M, Busch H, Chmel N, Kurz T, Bruckner-Tuderman L, Has C
    Hum. Mutat., 2011;32(4):397-406.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  3. The Th17 cytokine IL-22 induces IL-20 production in keratinocytes: a novel immunological cascade with potential relevance in psoriasis.
    Authors: Wolk K, Witte E, Warszawska K, Schulze-Tanzil G, Witte K, Philipp S, Kunz S, Docke WD, Asadullah K, Volk HD, Sterry W, Sabat R
    Eur. J. Immunol., 2009;39(12):3570-81.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
Isotype Controls
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

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