|Detection of Human IL‑22 by Western Blot. Western blot shows lysates of human tonsil tissue and human breast cancer tissue. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human IL‑22 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF782) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for IL‑22 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.|
|IL‑22 in Human PBMCs. IL‑22 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) treated with lipopolysaccharide (LPS) using 10 µg/mL Goat Anti-Human IL‑22 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF782) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.|
IL‑10 Secretion Induced by IL‑22 and Neutralization by Human IL‑22 Antibody. |
Recombinant Human IL‑22 (Catalog # 782-IL) stimulates IL‑10 secretion in the COLO 205 human colorectal adenocarcinoma cell line in a dose-dependent manner (orange line), as measured by the Human IL‑10 DuoSet ELISA Development Kit (Catalog # DY217B). IL‑10 secretion elicited by Recombinant Human IL‑22 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑22 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF782). The ND50 is typically 0.5-2.5 µg/mL.
Interleukin-22 (IL-22), also known as IL-10-related T cell-derived inducible factor (IL-TIF) was initially identified as a gene induced by IL-9 in mouse T cells and mast cells. Human IL-22 cDNA encodes a 179 amino acid (aa) residue protein with a putative 33 aa signal peptide that is cleaved to generate a 147 aa mature protein that shares approximately 79% and 22% aa sequence identity with mouse IL-22 and human IL-10, respectively. The human IL-22 gene is localized to chromosome 12q15. Although it exists as a single copy gene in human and in many mouse strains, the mouse IL-22 gene is duplicated in some mouse strains including C57B1/6, FVB and 129. The two mouse genes designated IL-TIF alpha and IL-TIF beta, share greater than 98% sequence homology in their coding region. IL-22 has been shown to activate STAT1 and STAT3 in several hepatoma cell lines and upregulate the production of acute phase proteins. IL-22 is produced by normal T cells upon anti-CD3 stimulation in humans. Mouse IL-22 expression is also induced in various organs upon lipopolysaccharide injection, suggesting that IL-22 may be involved in inflammatory responses. The functional IL-22 receptor complex consists of two receptor subunits, IL-22 R (previously an orphan receptor named CRF2-9) and IL-10 R beta (previously known as CRF2-4), belonging to the class II cytokine receptor family.
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