Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Neutralization, Immunocytochemistry

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Neutralization

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all IL-22 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human IL-22
Ala34-Ile179
Accession # Q9GZX6

Specificity

Detects human, recombinant mouse, and recombinant rat IL-22 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human IL-10 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human IL‑22 Antibody

Detection of Human IL-22 antibody by Western Blot.

Detection of Human IL‑22 by Western Blot.

Western blot shows lysates of human tonsil tissue and human breast cancer tissue. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human IL-22 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF782) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for IL-22 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

IL‑22 antibody in Human PBMCs by Immunocytochemistry (ICC).

IL‑22 in Human PBMCs.

IL-22 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) treated with lipopolysaccharide (LPS) using 10 µg/mL Goat Anti-Human IL-22 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF782) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

IL‑10 Secretion Induced by IL‑22 and Neutralization by Human IL‑22 Antibody.

IL‑10 Secretion Induced by IL‑22 and Neutralization by Human IL‑22 Antibody.

Recombinant Human IL-22 (Catalog # 782-IL) stimulates IL-10 secretion in the COLO 205 human colorectal adeno-carcinoma cell line in a dose-dependent manner (orange line), as measured by the Human IL-10 DuoSet ELISA Development Kit (Catalog # DY217B). IL-10 secretion elicited by Recombinant Human IL-22 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-22 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF782). The ND50 is typically 0.5-2.5 µg/mL.

Detection of Human IL-22 by Western Blot

Detection of Human IL-22 by Western Blot

Distribution, expression and correlation of IL-22 cells with liver fibrosis in the liver.(A) IL-22 positive cells mainly located in portal areas with brownish-yellow color by immunohistochemistry. (B) Immunohistochemistry double staining identities IL-22 cells were produced by CD4 cells in liver tissue. Black arrows indicate double-positive cells. The frequencies of IL-22 cells (C) and the expression of IL-22 mRNA (D) in the liver tissue were significantly higher in HCV-OLT patients, compared to the OLT and HCV patients. (E) Protein in the liver also had the same change by Western-blotting. IL-22 cells are positively correlated with alpha -SMA (F) and fibrosis staging (G), not with grading score (H) and HCVRNA (I). *P < 0.05, **P < 0.01, ***P < 0.001, **** P < 0.0001, ns p>0.05. Image collected and cropped by CiteAb from the following open publication (https://dx.plos.org/10.1371/journal.pone.0154419), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human IL-22 by Western Blot

Detection of Human IL-22 by Western Blot

Distribution, expression and correlation of IL-22 cells with liver fibrosis in the liver.(A) IL-22 positive cells mainly located in portal areas with brownish-yellow color by immunohistochemistry. (B) Immunohistochemistry double staining identities IL-22 cells were produced by CD4 cells in liver tissue. Black arrows indicate double-positive cells. The frequencies of IL-22 cells (C) and the expression of IL-22 mRNA (D) in the liver tissue were significantly higher in HCV-OLT patients, compared to the OLT and HCV patients. (E) Protein in the liver also had the same change by Western-blotting. IL-22 cells are positively correlated with alpha -SMA (F) and fibrosis staging (G), not with grading score (H) and HCVRNA (I). *P < 0.05, **P < 0.01, ***P < 0.001, **** P < 0.0001, ns p>0.05. Image collected and cropped by CiteAb from the following open publication (https://dx.plos.org/10.1371/journal.pone.0154419), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human Human IL-22 Antibody by Immunohistochemistry

Detection of Human Human IL-22 Antibody by Immunohistochemistry

IL-22 and IL-22R1 are expressed in human brain. IL-22, IL-22R1, GFAP, and Caveolin-1 immunohistochemistry peroxidase stainings of brain tissue sections of control (a) and MS (b–d) patients. MOG and HE stainings were used to detect MS demyelinating plaques (d). All four pictures belonging to one column (a, b, c, or d) were always immediately adjacent to each other. Pictures a, b, and c were taken at areas at the border between GM and NAWM, whereas pictures in d were taken from the same location at the edge between NAWM and a plaque. Inserts in columns a and b represent a threefold magnification of the selected area. Arrow: astrocyte-like pattern. a study patient B-C2, b and d study patient B-MS3, c study patient B-MS5 (Table 2). Scale bar, 50 μm (a, b: ×20, c, d ×40). GM: gray matter, NAWM: normal appearing white matter, WM: white matter. Representative pictures obtained from the observations of seven control and five MS autopsy samples Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26077779), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human IL‑22 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs) treated with lipopolysaccharide (LPS)

Western Blot

1 µg/mL
Sample: Human tonsil tissue and human breast cancer tissue

Neutralization

Measured by its ability to neutralize IL‑22-induced IL‑10 secretion in the COLO 205 human colorectal adenocarcinoma cell line [Marehalli, L. et al. (2004) Intl. Immunopharmacol. 4:679]. The Neutralization Dose (ND50) is typically 0.5-2.5 µg/mL in the presence of 1 ng/mL Recombinant Human IL‑22.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-22

Interleukin-22 (IL-22), also known as IL-10-related T cell-derived inducible factor (IL-TIF) was initially identified as a gene induced by IL-9 in mouse T cells and mast cells. Human IL-22 cDNA encodes a 179 amino acid (aa) residue protein with a putative 33 aa signal peptide that is cleaved to generate a 147 aa mature protein that shares approximately 79% and 22% aa sequence identity with mouse IL-22 and human IL-10, respectively. The human IL-22 gene is localized to chromosome 12q15. Although it exists as a single copy gene in human and in many mouse strains, the mouse IL-22 gene is duplicated in some mouse strains including C57B1/6, FVB and 129. The two mouse genes designated IL-TIF alpha and IL-TIF beta, share greater than 98% sequence homology in their coding region. IL-22 has been shown to activate STAT1 and STAT3 in several hepatoma cell lines and upregulate the production of acute phase proteins. IL-22 is produced by normal T cells upon anti-CD3 stimulation in humans. Mouse IL-22 expression is also induced in various organs upon lipopolysaccharide injection, suggesting that IL-22 may be involved in inflammatory responses. The functional IL-22 receptor complex consists of two receptor subunits, IL-22 R (previously an orphan receptor named CRF2-9) and IL-10 R beta (previously known as CRF2-4), belonging to the class II cytokine receptor family.

References

  1. Dumoutier, L. et al. (2000) J. Immunol. 164:1814.
  2. Xie, M-H. et al. (2000) J. Biol. Chem. 275:31335.
  3. Dumoutier, L. et al. (2000) Proc. Natl. Acad. Sci. USA 97:10144.
  4. Kotenko, S.V. et al. (2001) J. Biol. Chem. 276:2725.

Long Name

Interleukin 22

Alternate Names

IL-TIF, IL22

Entrez Gene IDs

50616 (Human); 50929 (Mouse); 500836 (Rat)

Gene Symbol

IL22

UniProt

Q9GZX6

Additional IL-22 Products

Product Documents for Human IL‑22 Antibody

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Product Specific Notices for Human IL‑22 Antibody

For research use only

Citations for Human IL‑22 Antibody

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Protocols

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