Human IL-22BP Antibody

Catalog # Availability Size / Price Qty
MAB10871
MAB10871-SP
Human IL-22BP Antibody in Intracellular Staining by Flow Cytometry
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Human IL-22BP Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-22BP in direct ELISAs. In direct ELISAs, no cross-reactivity with mouse IL-22BP is observed.
Source
Monoclonal Mouse IgG2B Clone # 875504
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IL-22BP
Thr22-Pro263
Accession # Q969J5
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection of IL‑22BP in Human (PBMC) Monocytes by Flow Cytometry. View Larger

Detection of IL‑22BP in Human (PBMC) Monocytes by Flow Cytometry. Human peripheral blood mononuclear cell (PBMC) monocytes were stained with Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P) and either (A) Mouse Anti-Human IL-22BP Monoclonal Antibody (Catalog # MAB10871) or (B) Mouse IgG2BFlow Cytometry Isotype Control (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-22BP

Interleukin 22 Binding Protein (IL-22BP), also known as Cytokine Receptor Family (CRF) 2‑10, CRF2-X, and IL-22 RA2, is a secreted glycoprotein belonging to the type II cytokine receptor family. It encodes a precursor protein of 231 amino acid (aa) residues with a 21 aa putative signal peptide and five potential N-linked glycosylation sites. IL-22BP lacks a transmembrane and cytoplasmic domain and is most closely related to the extracellular domains of IL‑22 R (CRF2-9) and IL-20 R (CRF2-8), sharing 33% and 34% aa sequence identity, respectively. It also shares sequence homology with the extracellular domains of IL-10 R (29%), IL-10 R beta (30%), the IFN receptors (23‑25%) and tissue factor (26%). IL-22BP antagonizes IL-22 activity by specifically binding IL-22 with high affinity and blocking its interaction with the cell surface IL-22 receptor heteromeric complex composed IL-22 R and IL‑20 R. IL-22BP is expressed in multiple tissues. The highest levels of expression are found in breast, lungs and colon. The major cell types producing IL-22BP are monocytes, activated B cells and epithelial cells (1), and is constitutively expressed by a subset of conventional dendritic cells (2). IL-22BP is regulated by the inflammasome and it has been suggested to modulate tumorigenesis in the intestine (3).

References
  1. Xu, W. et al. (2001) Proc. Natl. Acad. Sci. USA 98:9511.
  2. Martin, J. et al. (2014)  Mucosal Immunol. 7:101.
  3. Huber, S. et al. (2012)  Nature 491:259.
    Long Name
    Interleukin 22 Binding Protein
    Entrez Gene IDs
    116379 (Human); 237310 (Mouse)
    Alternate Names
    class II cytokine receptor; CRF2-10; CRF2-S1IL22BP; CRF2-X; Cytokine receptor class-II member 10; Cytokine receptor family 2 member 10; Cytokine receptor family type 2, soluble 1; IL-22 RA2; IL-22 receptor subunit alpha-2; IL22BP; IL-22BP; IL-22BPCRF2X; IL22RA2; IL-22RA2; IL-22R-alpha-2; interleukin 22 receptor, alpha 2; interleukin 22-binding protein; interleukin-22 receptor subunit alpha-2; Interleukin-22-binding protein; MGC150509; MGC150510; zcytoR16

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    Isotype Controls

    Reconstitution Buffers

    Secondary Antibodies

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