Human IL-22BP Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human IL-22BP in direct ELISAs. In direct ELISAs, no cross-reactivity with mouse IL-22BP is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 875504
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human IL-22BP
    Thr22-Pro263
    Accession # Q969J5
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Intracellular Staining by Flow Cytometry
    2.5 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of IL‑22BP in Human (PBMC) Monocytes by Flow Cytometry. Human peripheral blood mononuclear cell (PBMC) monocytes were stained with Mouse Anti-Human CD14 PE‑conjugated Monoclonal Antibody (Catalog # FAB3832P) and either (A) Mouse Anti-Human IL‑22BP Monoclonal Antibody (Catalog # MAB10871) or (B) Mouse IgG2B Flow Cytometry Isotype Control (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-22BP

Interleukin 22 Binding Protein (IL-22BP), also known as Cytokine Receptor Family (CRF) 2‑10, CRF2-X, and IL-22 RA2, is a secreted glycoprotein belonging to the type II cytokine receptor family. It encodes a precursor protein of 231 amino acid (aa) residues with a 21 aa putative signal peptide and five potential N-linked glycosylation sites. IL-22BP lacks a transmembrane and cytoplasmic domain and is most closely related to the extracellular domains of IL‑22 R (CRF2-9) and IL-20 R (CRF2-8), sharing 33% and 34% aa sequence identity, respectively. It also shares sequence homology with the extracellular domains of IL-10 R (29%), IL-10 R beta (30%), the IFN receptors (23‑25%) and tissue factor (26%). IL-22BP antagonizes IL-22 activity by specifically binding IL-22 with high affinity and blocking its interaction with the cell surface IL-22 receptor heteromeric complex composed IL-22 R and IL‑20 R. IL-22BP is expressed in multiple tissues. The highest levels of expression are found in breast, lungs and colon. The major cell types producing IL-22BP are monocytes, activated B cells and epithelial cells (1), and is constitutively expressed by a subset of conventional dendritic cells (2). IL-22BP is regulated by the inflammasome and it has been suggested to modulate tumorigenesis in the intestine (3).

  • References:
    1. Xu, W. et al. (2001) Proc. Natl. Acad. Sci. USA 98:9511.
    2. Martin, J. et al. (2014)  Mucosal Immunol. 7:101.
    3. Huber, S. et al. (2012)  Nature 491:259.
    1. Long Name:
      Interleukin 22 Binding Protein
    2. Entrez Gene IDs:
      116379 (Human); 237310 (Mouse)
    3. Alternate Names:
      class II cytokine receptor; CRF2-10; CRF2-S1IL22BP; CRF2-X; Cytokine receptor class-II member 10; Cytokine receptor family 2 member 10; Cytokine receptor family type 2, soluble 1; IL-22 RA2; IL-22 receptor subunit alpha-2; IL22BP; IL-22BP; IL-22BPCRF2X; IL22RA2; IL-22RA2; IL-22R-alpha-2; interleukin 22 receptor, alpha 2; interleukin 22-binding protein; interleukin-22 receptor subunit alpha-2; Interleukin-22-binding protein; MGC150509; MGC150510; zcytoR16
    Related Research Areas
    Isotype Controls
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    Mouse IgG2B Isotype Control

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    Mouse IgG2B Isotype Control

    Ctrl MAB0042 6
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    Secondary Antibodies
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    Flow F0102B 10  
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    Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

    Flow, IHC, ICC NL007 9
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    Donkey Anti-Mouse IgG Biotinylated Antibody

    WB BAF018 1
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    Mouse IgG HRP-conjugated Antibody

    WB HAF018 9  
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    Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

    Flow, IHC, ICC NL008 8
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    Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

    Flow F0101B 2  
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    Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

    Flow, IHC, ICC NL009 5
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    Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

    Flow F0103B 5  
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    Mouse IgG Antibody

    WB AF007 4
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    Goat Anti-Mouse IgG Biotinylated Antibody

    WB BAF007 4
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    Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

    IHC VC002  
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    Mouse IgG VisUCyte HRP Polymer Antibody

    IHC VC001  
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    Rat Anti-Mouse IgG2B APC-conjugated Antibody

    Flow F0133 1  
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    Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

    G-202-C 2
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    Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

    Flow F0114  
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    Rat Anti-Mouse IgG2B Fluorescein-conjugated Antibody

    Flow F0134 1
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    Rat Anti-Mouse IgG2B PE-conjugated Antibody

    Flow F0132  
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    Mouse IgG2B Antibody

    Flow MAB0043  
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