Human IL-26/AK155 Antibody (197505)

Detection of IL-26/AK155 by Immunohistochemistry

IL-26 expression in peripheral joints is increased in axSpA. (A) Representative slides of IL-26 expression by immunohistochemistry in peripheral synovial tissue from HCs (n = 5; white) and axSpA (n = 9; black), PsA (n = 3; red), and RA (n = 7; blue) patients. Isotype staining in 3,3′diaminobenzidine (brown) is shown on the far left. 20× (scale bar 500 μm) and inlay 80× (scale bar 50 μm). (B) Semiquantitative analysis of IL-26+ pixels in peripheral joint tissue sections from the cohort described in (A). Statistical analysis (B): mean ± SEM, **p < 0.01 (Kruskal–Wallis test). Image collected and cropped by CiteAb from the following open publication (https://www.frontiersin.org/articles/10.3389/fimmu.2024.1355824/full), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of IL-26/AK155 by Flow Cytometry

IL26-expressing cells are only slightly increased in circulating CD4+ and CD8+ T cells from patients with axSpA and PsA. (A) Representative flow cytometry plots of total IL26+CD4+ T cells with the PrimeFlow assay in a PsA patient. RPLA13A represents the positive control for the assay. (B) Percentages of total IL26+CD4+ T cells (left) and percentages of IL26+ cells of CD26+CD161+CD4+ T cells (right) for HCs (n = 15; white) and axSpA (n = 15; black) and PsA (n = 14; red) patients gated from PBMCs stimulated in vitro with PMA and ionomycin in the PrimeFlow assay. (C) Representative flow cytometry plots of total IL-1R1+ Th17 cells in a PsA patient comparing IL-2 stimulation to unstimulated conditions. (D) Percentages of IL-1R1+ Th17 cells (Th17 cells gated as CD4+CD45RO+CD161+CCR6+CCR4+CXCR3−) of CD4+ cells from HCs (n = 21; white) and axSpA (n = 22; black), PsA (n = 14; red), and RA (n = 13; blue) patients after in vitro stimulation with IL-2 (1,000 IU/ml) overnight (left) and percentages of IL-1R1+CD26+CD161+ cells of CD4+ cells (right). (E) Percentages of total IL26+CD8+ T cells (left) and percentages of IL26+ cells of CD26+CD161+CD8+ T cells (right) for HCs (n = 15; white) and axSpA (n = 15; black) and PsA (n = 14; red) patients gated from PBMCs stimulated in vitro with PMA and ionomycin. Statistical analysis: mean ± SEM, *p < 0.05, **p < 0.01 (one-way ANOVA followed by Dunnett post-test for multiple comparisons). Image collected and cropped by CiteAb from the following open publication (https://www.frontiersin.org/articles/10.3389/fimmu.2024.1355824/full), licensed under a CC-BY license. Not internally tested by R&D Systems.