Interleukin 3 is a pleiotropic cytokine produced primarily by activated T cells or mast cells. IL-3 stimulates the proliferation and differentiation of hemopoietic cells including the pluripotent hematopoietic stem cells as well as various lineage-committed cells. The biological effects of IL-3 on the various cell types are mediated by the binding of IL-3 to specific cell surface receptor complexes. The functional high-affinity human IL-3 receptor is a heterodimer consisting of a ligand binding alpha subunit and the beta subunit. The alpha subunit alone binds IL-3 with low affinity. The beta subunit does not bind IL-3 by itself but is required for the high-affinity binding of IL-3 to the heterodimeric receptor complex. The beta subunit has also been found to be a component of the high-affinity receptor complex for IL-5 and GM-CSF. Both the alpha and the beta subunits are members of the cytokine receptor superfamily.
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Scientific Data Images for Human IL-3R alpha/CD123 Antibody
Detection of Human IL-3R alpha/CD123 by Western Blot.
Western blot shows lysates of HDLM‑2 human Hodgkin’s lymphoma cell line, TF‑1 human erythroleukemic cell line, and THP‑1 human acute monocytic leukemia cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human IL-3R alpha/CD123 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF841) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for IL-3R alpha/CD123 at approximately 70-75 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Detection of Human IL-3R alpha/CD123 by Western Blot
Characteristics of sEV isolated from AML patients’ plasma at diagnosis: (A) transmission electron microscopy (TEM) of isolated sEV, (B) size and concentration of sEV as determined by NanoSight, (C) Western blots show that sEV carry endocytic markers (Alix, CD9, and TSG101) but not cytoplasmic proteins such as Grp94 or Calnexin, and (D) Western blots show the presence of LAAs in the isolated sEV. LAAs are differentially expressed in each patient’s sEV. Each lane was loaded with 10 μg of sEV protein. TSG101 is a marker that confirms the endocytic origin of sEV. NC: sEV isolated from healthy donors’ plasma. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38137457), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Human IL-3R alpha/CD123 Antibody
Western Blot
Sample: HDLM‑2 human Hodgkin's lymphoma cell line, TF‑1 human erythroleukemic cell line, and THP‑1 human acute monocytic leukemia cell line
Reviewed Applications
Read 3 reviews rated 4 using AF841 in the following applications:
Formulation, Preparation, and Storage
Purification
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: IL-3R alpha
References
- Ogorochi, T. and A. Miyajima (1994) in Guidebook to Cytokines and Their Receptors, N.A. Nicola, ed. Oxford University, New York p. 40.
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Additional IL-3R alpha Products
Product Documents for Human IL-3R alpha/CD123 Antibody
Product Specific Notices for Human IL-3R alpha/CD123 Antibody
For research use only
Citations for Human IL-3R alpha/CD123 Antibody
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Application: MicroarraysSample Tested: EDTA PlasmaSpecies: HumanVerified Customer | Posted 06/10/2020
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Application: MicroarraysSample Tested: EDTA PlasmaSpecies: HumanVerified Customer | Posted 11/07/2018
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Application: MicroarraySample Tested: EDTA PlasmaSpecies: HumanVerified Customer | Posted 11/02/2018
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
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