Human IL-31 RA Antibody

Catalog # Availability Size / Price Qty
AF2769
AF2769-SP
Detection of IL‑31RA in U937 Human Cell Line by Flow Cytometry.
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Product Details
Citations (3)
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Human IL-31 RA Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-31 RA in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross‑reactivity with recombinant mouse
IL‑31 RA is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IL-31 RA
Ala20-Ser516
Accession # Q8NI17
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human IL‑31 RA (Catalog # 2769-IL)
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of IL‑31RA in U937 Human Cell Line by Flow Cytometry. View Larger

Detection of IL‑31RA in U937 Human Cell Line by Flow Cytometry. U937 human histiocytic lymphoma cell line was stained with Goat Anti-Human IL‑31 RA Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF2769, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histo­gram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Immunocytochemistry IL‑31 RA  in K562 Human Cell Line. View Larger

IL‑31 RA in K562 Human Cell Line.
IL‑31 RA was detected in immersion fixed K562 human chronic myelogenous leukemia cell line using Goat Anti-Human IL‑31 RA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2769) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunocytochemistry IL‑31 RA  in THP‑1 Human Cell Line. View Larger

IL‑31 RA in THP‑1 Human Cell Line.
IL‑31 RA was detected in immersion fixed THP‑1 human acute monocytic leukemia cell line using Goat Anti-Human IL‑31 RA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2769) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-31 RA

The interleukin-31 receptor A subunit (IL-31 RA), also known as gp130-Like Monocyte Receptor (GLM-R or GPL), is a ~100 kDa type I transmembrane glycoprotein that is classified as being a type I cytokine receptor (1, 2). A heterodimeric complex of IL-31 RA and the oncostatin M receptor (OSM-R) functions as the signaling receptor for IL-31 (3). Both subunits are inducibly expressed throughout the myelomonocytic lineage and are upregulated by interferon-gamma and bacterial lipopolysaccharides (1‑3). IL-31 RA is also expressed on keratinocytes, dorsal root ganglia neurons, and variably on lung epithelial cells (3‑6). The 732 amino acid (aa) IL-31 RA contains a 19 aa signal sequence, a 500 aa extracellular domain (ECD), a 21 aa transmembrane domain and a 192 aa cytoplasmic domain. The ECD shares 60%, 58%, 73% and 70% aa identity with mouse, rat, canine and bovine IL-31 RA ECD, respectively. Human IL-31 receptors do not respond to mouse IL-31 (7). The ECD contains five fibronectin type III domains; the first two contain four conserved cysteine residues and a WSXWS motif common to type I cytokine receptors (2). Twelve alternately spliced human IL-31 RA isoforms are known and range in size from 356‑745 amino acids. A long (745 aa) and a short (560 aa) transmembrane form are the predominant forms, and many cell lines express both forms (8). The long form, like the 732 aa form, signals by recruiting STAT3, 5 or 1, while the short form does not recruit STATs and inhibits IL-31 signaling. The ratio of these forms and their co-expression with OSM-R determines a cell’s response to IL‑31 (8). In both humans and transgenic mice, IL-31 from skin-homing Th2 cells may contribute to the pruritis (itching) associated with nonatopic dermatitis, especially in infected skin (3, 9, 10).

References
  1. Ghilardi, N. et al. (2002) J. Biol. Chem. 277:16831.
  2. Diveu, C. et al. (2003) J. Biol. Chem. 278:49850.
  3. Dillon, S. R. et al. (2004) Nat. Immunol. 5:752.
  4. Chattopadhyay, S. et al. (2007) J. Biol. Chem. 282:3014.
  5. Perrigoue, J. G. et al. (2007) J. Exp. Med. 204:481.
  6. Bando, T. et al. (2006) Neuroscience 142:1263.
  7. Broxmeyer, H. E. et al. (2007) Exp. Hematol. 35:78.
  8. Diveu, C. et al. (2004) Eur. Cytokine. Netw. 15:291.
  9. Bilsborough, J. et al. (2006) J. Allergy Clin. Immunol. 117:418.
  10. Sonkoly, E. et al. (2006) J. Allergy Clin. Immunol. 117:411.
Long Name
Interleukin 31 Receptor A
Entrez Gene IDs
133396 (Human); 218624 (Mouse)
Alternate Names
class I cytokine receptor; CRL3; CRL3Glmr; Cytokine receptor-like 3; GLMR; GLM-R; GLM-RMGC125346; GP130 like receptor; Gp130-like monocyte receptor; Gp130-like receptor; hGLM-R; IL-31 RA; IL-31 receptor subunit alpha; IL-31R subunit alpha; IL31RA; IL-31RA; IL-31RAGPLCRL; IL-31R-alpha; interleukin 31 receptor A; interleukin-31 receptor subunit alpha; PRO21384; soluble type I cytokine receptor CRL3; zcytoR17

Product Datasheets

Citations for Human IL-31 RA Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Unbiased classification of sensory neuron types by large-scale single-cell RNA sequencing.
    Authors: Usoskin D, Furlan A, Islam S, Abdo H, Lonnerberg P, Lou D, Hjerling-Leffler J, Haeggstrom J, Kharchenko O, Kharchenko P, Linnarsson S, Ernfors P
    Nat Neurosci, 2015;18(1):145-53.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. A sensory neuron-expressed IL-31 receptor mediates T helper cell-dependent itch: Involvement of TRPV1 and TRPA1.
    Authors: Cevikbas F, Wang X, Akiyama T, Kempkes C, Savinko T, Antal A, Kukova G, Buhl T, Ikoma A, Buddenkotte J, Soumelis V, Feld M, Alenius H, Dillon S, Carstens E, Homey B, Basbaum A, Steinhoff M
    J Allergy Clin Immunol, 2014;133(2):448-60.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  3. Interleukin 31 mediates MAP kinase and STAT1/3 activation in intestinal epithelial cells and its expression is upregulated in inflammatory bowel disease.
    Authors: Dambacher J, Beigel F, Seiderer J, Haller D, Goke B, Auernhammer CJ, Brand S
    Gut, 2007;56(9):1257-65.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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