Human IL-36 alpha /IL-1F6 Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB10782-100
MAB10782-SP

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Human IL‑36 alpha /IL‑1F6 ELISA Standard Curve.
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Human IL-36 alpha /IL-1F6 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-36 alpha/IL-1F6 in direct ELISAs.
Source
Recombinant Monoclonal Rabbit IgG Clone # 1087A
Purification
Protein A or G purified from cell culture supernatant
Immunogen
E. coli-derived human IL-36 alpha/IL-1F6
Lys6-Phe158
Accession # NP_055255
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
ELISA

This antibody functions as an ELISA detection antibody when paired with Rabbit Anti-Human IL‑36 alpha /IL‑1F6 Monoclonal Antibody (Catalog # MAB10783).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human IL-36 alpha/IL-1F6 DuoSet ELISA Kit (Catalog # DY1078-05) for convenient development of a sandwich ELISA.

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Human IL‑36 alpha /IL‑1F6 ELISA Standard Curve. Recombinant Human IL-36a/IL-1F6 protein was serially diluted 2-fold and captured by Rabbit Anti-Human IL-36a/IL-1F6 Monoclonal Antibody (Catalog # MAB10783) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rabbit Anti-Human IL-36a/IL-1F6 Monoclonal Antibody (Catalog # MAB10782) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-36 alpha/IL-1F6

Human IL-36 alpha, previously called IL-1F6 and FIL1 epsilon  (family of IL-1 member epsilon), is a member of the IL-1 family which includes IL-1 beta, IL-1 alpha, IL-1ra, IL-18, and novel family members IL-36 Ra (IL-1F5), IL-36 beta (IL-1F8), IL-36 gamma (IL-1F9), IL-37 (IL-1F7) and IL‑38 (IL‑1F10) (1‑4). All family members show a 12  beta ‑strand, beta -trefoil configuration, and are believed to have arisen from a common ancestral gene (1, 2). IL-36 alpha is an 18‑22 kDa, 158 amino acid (aa) intracellular and secreted protein that contains no signal sequence, no prosegment and no potential from N‑linked glycosylation sites (1‑3). It can be released in response to LPS and the cell ATP‑induced activation of the P2X7 receptor (5). A 120 aa isoform missing aa 1‑38 has been reported (6). Human IL‑36 alpha (aa 6 ‑ 158) shares 57‑68% aa sequence identity with mouse, rabbit, equine and bovine IL‑36 alpha and 27‑57% aa sequence identity with other novel IL‑1 family members. IL‑36 alpha is mainly found in skin and lymphoid tissues, but also in fetal brain, trachea, stomach and intestine (1, 3, 7). It is expressed by monocytes, B and T cells (1, 2). The receptor for IL‑36 alpha is a combination of IL‑1 Rrp2 (also called IL1RL2 or IL‑1 R6), mainly found in epithelia and keratinocytes, and the widely expressed IL‑1 RAcP (3, 7). IL-36 alpha, beta, and gamma all activate NF-kappa B and MAPK pathways in an IL‑1 Rrp2 dependent manner, and induce production of inflammatory cytokines and chemokines such as CXCL8/IL-8 (7). IL-36 alpha and other family members are overexpressed in psoriatic skin lesions, and transgenic overexpression of IL‑36 alpha in skin keratinocytes produces epidermal hyperplasia (7‑9). IL-36 alpha is present in kidney tubule epithelia, and it is highly expressed in intubulointerstitial lesions in mouse models of chronic glomerulonephritis, lupus nephritis and diabetic nephritis (10).

References
  1. Smith, D.E. et al. (2000) J. Biol. Chem. 275:1169.
  2. Dunn, E. et al. (2001) Trends Immunol. 22:533.
  3. Barksby, H.E. et al. (2007) Clin. Exp. Immunol. 149:217.
  4. Dinarello, C. et al. (2010) Nat. Immunol. 11:973.
  5. Martin, U. et al. (2009) J. Immunol. 183:4021.
  6. Entrez Accession # EAW73614.
  7. Towne, J.E. et al. (2004) J. Biol. Chem. 279:13677.
  8. Blumberg, H. et al. (2010) J. Immunol. 185:4354.
  9. Johnston, A. et al. (2011) J. Immunol. 186:2613.
  10. Ichii, O. et al. (2010) Lab. Invest. 90:459.
Long Name
Interleukin 36 alpha/Interleukin 1 Family 6
Entrez Gene IDs
27179 (Human); 54448 (Mouse)
Alternate Names
FIL1 epsilon; FIL1; FIL1(EPSILON); FIL1E; IL-1 epsilon; IL1(EPSILON); IL1E; IL-1E; IL-1F6 (FIL-1-epsilon); IL1F6; IL-1F6; IL36 alpha; IL-36 alpha; IL36A; interleukin 1 family, member 6 (epsilon); interleukin 1, epsilon; Interleukin 36, Alpha; Interleukin-1 epsilon; interleukin-1 family member 6; Interleukin-36 Alpha

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