Human IL-37/IL-1F7 Antibody

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Detection of Human IL-37/IL-1F7 by Western Blot
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Product Details
Citations (2)
Supplemental Products

Human IL-37/IL-1F7 Antibody Summary

Species Reactivity
Detects human IL-37/IL-1F7 in direct ELISAs. In direct ELISAs, no cross‑reactivity with recombinant human (rh) IL-1 alpha, rhIL-1 beta, rhIL-1ra, rhIL-36Ra/IL-1F5, rhIL-36 alpha/IL-1F6, rhIL-36 beta/IL-1F8, or rhIL-18/IL-1F4 is observed.
Monoclonal Mouse IgG2B Clone # 261506
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human IL-37/IL-1F7
Accession # NP_775297
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.


Recommended Concentration
Blockade of Receptor-ligand Interaction
In a functional ELISA, 2-6 µg/mL of this antibody will block 50% of the binding of 1 μg/mL of Recombinant Human IL-18 R alpha /IL-1 R5 Fc Chimera (Catalog # 816-LR) to immobilized Recombinant Human IL-37/IL-1F7 (Catalog # 1975-IL) coated at 100 ng/mL (100 µL/well). At 50 μg/mL, this antibody will block >90% of the binding.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human IL-37/IL-1F7 by Western Blot View Larger

Detection of Human IL-37/IL-1F7 by Western Blot IL-37d inhibited pro-inflammatory cytokines expression.a–c A549 cells were transfected with the mock, IL-37b, or IL-37d recombinant plasmid, respectively, and stimulated with or without IL-1 beta (10 ng/ml) for the indicated times. The expressions of IL-37b and IL-37d were detected by RT-PCR (a) and western blot (b). The levels of IL-6 in the cell culture supernatants were determined by ELISA (c). The expression of IL-37d in bone marrow-derived macrophages (BMDMs) from wild-type and IL-37dtg mouse was detected by RT-PCR (d). BMDMs were stimulated with or without LPS (100 ng/ml) for 3 h, 6 h, or 9 h. The mRNA levels of IL-1 alpha, IL-1 beta, IL-6, and TNF-alpha were determined by qRT-PCR (e–h) and the levels of IL-6 and TNF in the cell culture supernatants were determined by cytometric beads array (CBA) (i, j). ND, Not detectable. *P < 0.05; **P < 0.01; ***P < 0.001, ns, no significant difference. Data are shown as the mean ± SEM from three independent experiments Image collected and cropped by CiteAb from the following publication (, licensed under a CC-BY license. Not internally tested by R&D Systems.

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-37/IL-1F7

Human interleukin 1 family member 7 (IL-1F7), also named FIL-1Z, IL-1H4, and IL-1RP1, belongs to the IL-1 cytokine family, which currently has ten members. With the exception of IL-18 that maps to human chromosome 11, all other IL-1 family members map to the same cluster on human chromosome 2. Five alternatively spliced transcripts that arise through alternate exon usage have been described. These transcripts encode five different IL-1F7 isoforms (IL-1F7a through e also referred to as isofoms 1 through 5) that have distinct expression profiles. Polymorphism in the protein sequence of IL-1F7 isoforms also exists. Like IL-1 alpha, IL-1 beta and IL-18, all of the IL-1F7 variants lack a typical signal peptide. The longest IL-1F7 transcript, referred to as IL-1F7b or IL-1F7 isoform 1, encodes a 218 amino acid (aa) residues proprotein containing a 45 aa propeptide, which is removed by caspase-1 to generate the 173 aa mature segment. Mature IL-1F7b and other IL-1F7 variants lack potential N-linked glycosylation sites. The secreted mature IL-17F7b was reported to exist as a nondisulfide linked homodimers in solution, IL-1F7 shares approximately 21%, 24%, and 30% aa sequence identity with mature IL-1 alpha, IL-1 beta and IL-1ra, respectively. Mouse IL-1F7 has not been reported, but human IL-1F7 is active on mouse cells. IL-1F7b binds to IL-18 R alpha with low affinity but does not exert any IL-18 agonistic or antagonistic effects. IL-1F7b also binds to the IL-18BP to enhance the antagonistic effects of IL-18BP. It has been proposed that IL-1F7b form a trimeric complex with IL-18BP and IL-18 R beta. This complex blocks IL-18 activity by sequestering the signal transducing subunit and preventing it from participating in IL-18 signaling (1‑8).

  1. Smith, D.E. et al. (2000) J. Biol. Chem. 275:1169.
  2. Kumar S. et al. (2000) J. Biol. Chem. 275:10308.
  3. Busfield S.J. et al. (2000) Genomics 66:213.
  4. Kumar S. et al. (2002) Cytokine 18:61.
  5. Bufler, P. et al. (2002) Proc. Natl. Acad. Sci. USA 99:13723.
  6. Gao, W. et al. (2003) J. Immunol. 170:1077.
  7. Pan, G. et al. (2001) Cytokine 13:1.
  8. Sims, J.E. et al. (2001) Trends Immunol. 22:533.
Long Name
Interleukin 37
Entrez Gene IDs
27178 (Human)
Alternate Names
FIL1 zeta; FIL1; FIL1(ZETA); FIL1ZFIL1 zeta; IL-1F7; IL-1F7IL-1 zeta; IL1H4IL1F7 (canonical product IL-1F7b); IL-1H4IL-1F7b (IL-1H4, IL-1H, IL-1RP1); IL-1RP1IL-1X protein; IL1RP1interleukin 1 family member 7; IL-1X; IL37; IL-37; interleukin 1 family, member 7 (zeta); interleukin 1, zeta; interleukin-1 family member 7; Interleukin-1 homolog 4; interleukin-1 superfamily z; Interleukin-1 zeta; Interleukin-1-related protein

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Citations for Human IL-37/IL-1F7 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Interleukin-37 reduces inflammation and impairs phagocytosis of platelets in immune thrombocytopenia (ITP)
    Authors: Y Zhao, X Ni, P Xu, Q Liu, T Sun, X Liu, X Ji, J Qiu, J Li, S Wang, P Han, J Peng, M Hou, G Li
    Cytokine, 2019-09-23;125(0):154853.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  2. IL-37 isoform D downregulates pro-inflammatory cytokines expression in a Smad3-dependent manner
    Authors: M Zhao, Y Li, C Guo, L Wang, H Chu, F Zhu, Y Li, X Wang, Q Wang, W Zhao, Y Shi, W Chen, L Zhang
    Cell Death Dis, 2018-05-22;9(6):582.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization


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