Human IL-37/IL-1F7 Antibody Summary
Accession # Q9NZH6-2
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human IL-37/IL-1F7 by Western Blot. Western blot shows lysates of human peripheral blood mononuclear cells (PBMCs) untreated (-) or treated (+) with 2 µg/mL LPS for 24 hours and THP-1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 10 µg/mL LPS for 3 hours and 200 nM PMA for 24 hours. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human IL-37/IL-1F7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1975) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL-37/IL-1F7 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Human interleukin 1 family member 7 (IL-1F7), also named FIL-1Z, IL-1H4, and IL-1RP1, belongs to the IL-1 cytokine family, which currently has ten members. With the exception of IL-18 that maps to human chromosome 11, all other IL-1 family members map to the same cluster on human chromosome 2. Five alternatively spliced transcripts that arise through alternate exon usage have been described. These transcripts encode five different IL-1F7 isoforms (IL-1F7a through e also referred to as isofoms 1 through 5) that have distinct expression profiles. Polymorphism in the protein sequence of IL-1F7 isoforms also exists. Like IL-1 alpha, IL-1 beta and IL-18, all of the IL-1F7 variants lack a typical signal peptide. The longest IL-1F7 transcript, referred to as IL-1F7b or IL-1F7 isoform 1, encodes a 218 amino acid (aa) residues proprotein containing a 45 aa propeptide, which is removed by caspase-1 to generate the 173 aa mature segment. Mature IL-1F7b and other IL-1F7 variants lack potential N-linked glycosylation sites. The secreted mature IL-17F7b was reported to exist as a nondisulfide linked homodimers in solution, IL-1F7 shares approximately 21%, 24%, and 30% aa sequence identity with mature IL-1 alpha, IL-1 beta and IL-1ra, respectively. Mouse IL-1F7 has not been reported, but human IL-1F7 is active on mouse cells. IL-1F7b binds to IL-18 R alpha with low affinity but does not exert any IL-18 agonistic or antagonistic effects. IL-1F7b also binds to the IL-18BP to enhance the antagonistic effects of IL-18BP. It has been proposed that IL-1F7b form a trimeric complex with IL-18BP and IL-18 R beta. This complex blocks IL-18 activity by sequestering the signal transducing subunit and preventing it from participating in IL-18 signaling (1‑8).
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- Kumar S. et al. (2002) Cytokine 18:61.
- Bufler, P. et al. (2002) Proc. Natl. Acad. Sci. USA 99:13723.
- Gao, W. et al. (2003) J. Immunol. 170:1077.
- Pan, G. et al. (2001) Cytokine 13:1.
- Sims, J.E. et al. (2001) Trends Immunol. 22:533.
Citations for Human IL-37/IL-1F7 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 3
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Interleukin 37 promotes angiogenesis through TGF-? signaling
Authors: M Zhao, Y Hu, J Jin, Y Yu, S Zhang, J Cao, Y Zhai, R Wei, J Shou, W Cai, S Liu, X Yang, GT Xu, J Yang, DB Corry, SB Su, X Liu, T Yang
Sci Rep, 2017;7(1):6113.
Sample Types: Cell Lysates
Applications: Western Blot
IL-37 ameliorates the inflammatory process in psoriasis by suppressing proinflammatory cytokine production.
Authors: Teng X, Hu Z, Wei X, Wang Z, Guan T, Liu N, Liu X, Ye N, Deng G, Luo C, Huang N, Sun C, Xu M, Zhou X, Deng H, Edwards C, Chen X, Wang X, Cui K, Wei Y, Li J
J Immunol, 2014;192(4):1815-23.
Role of caspase-1 in nuclear translocation of IL-37, release of the cytokine, and IL-37 inhibition of innate immune responses.
Authors: Bulau A, Nold M, Li S, Nold-Petry C, Fink M, Mansell A, Schwerd T, Hong J, Rubartelli A, Dinarello C, Bufler P
Proc Natl Acad Sci U S A, 2014;111(7):2650-5.
Sample Types: In Vivo
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