Human IL-5 Antibody
Human IL-5 Antibody Summary
Accession # P05113
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Cell Proliferation Induced by IL‑5 and Neutralization by Human IL‑5 Antibody. Recombinant Human IL-5 (Catalog # 205-IL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human IL-5 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-5 Monoclonal Antibody (Catalog # MAB205). The ND50 is typically 0.01-0.03 µg/mL.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Interleukin 5 is a T cell-derived factor that promotes the proliferation, differentiation and activation of eosinophils. In mice, IL-5 has also been shown to be a growth and differentiation factor for B cells. Various names previously used to describe IL-5 include: T cell replacing factor (TRF), B cell growth factor II (BCGFII), B cell differentiation factor μ (BCDF μ), eosinophil differentiation factor (EDF) and eosinophil colony-stimulating factor (Eo-CSF). Biologically active IL-5 is a disulfide-linked homodimer. The cDNAs for murine and human IL-5 encode precursor proteins with signal peptides that are cleaved to form mature proteins containing 113 and 115 amino acid residues, respectively. Murine and human IL-5 are 70% identical in their amino acid sequences and show species cross‑reactivity. IL-5 exerts its activity on target cells by binding to specific cell surface receptors. The functional high‑affinity receptor for human IL-5 has been shown to be composed of a low-affinity IL-5 binding alpha -subunit and a non-binding common beta -subunit that is shared with the high‑affinity receptors for GM‑CSF and IL-3.
Citations for Human IL-5 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 3
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Inhibition of interleukin-5 induced false positive anti-drug antibody responses against mepolizumab through the use of a competitive blocking antibody
Authors: Daniel Sikkema
J. Immunol. Methods, 2016-11-24;0(0):.
Applications: Competitive Binding Assay
Human CD8+ T-cells recognizing peptides from Mycobacterium tuberculosis (Mtb) presented by HLA-E have an unorthodox Th2-like, multifunctional, Mtb inhibitory phenotype and represent a novel human T-cell subset.
Authors: van Meijgaarden K, Haks M, Caccamo N, Dieli F, Ottenhoff T, Joosten S
PLoS Pathog, 2015-03-24;11(3):e1004671.
Sample Types: Whole Cells
Regulation of the interleukin-5 receptor alpha-subunit on peripheral blood eosinophils from healthy subjects.
Authors: Hellman C, 107334, Hallden G, Hylander B, Lundahl J
Lagging strand gap suppression connects BRCA-mediated fork protection to nucleosome assembly through PCNA-dependent CAF-1 recycling, 2003-01-01;131(1):75-81.
Sample Types: Whole Cells
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