Human IL-6 R alpha Antibody

(1 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human IL‑6 R alpha in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant mouse IL-6 R alpha and recombinant human LIF is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Protein A or G purified
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑6 R alpha
    Leu20-Asp358
    Accession # P08887
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human IL‑6 R alpha (Catalog # 227-SR)
  • Neutralization
    Measured by its ability to neutralize IL‑6 R alpha -mediated inhibition of proliferation in the M1 mouse myeloid leukemia cell line. The Neutralization Dose (ND50) is typically 1-4 µg/mL in the presence of 30 ng/mL Recombinant Human IL‑6 R alpha and 30 ng/mL Recombinant Human IL‑6.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
IL‑6 R alpha  Enhancement of IL‑6‑dependent Inhibition of Cell Proliferation and Neutralization by Human IL‑6 R alpha  Antibody.

Recombinant Human IL‑6 R alpha (Catalog # 227-SR) enhances Recombinant Human IL‑6 (Catalog # 206-IL) inhibition of proliferation in the M1 mouse myeloid leukemia cell line in a dose-dependent manner (orange line). Enhancement of Recombinant Human IL‑6 (30 ng/mL) activity elicited by Recombinant Human IL‑6 R alpha (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑6 R alpha Polyclonal Antibody (Catalog #
AB‑227‑NA). The ND50 is typically 1‑4 µg/mL.

Preparation and Storage
  • Reconstitution
    Reconstitute at 1 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-6 R alpha

The multi-functional factor interleukin 6 (IL-6) exerts its activities through binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa component receptor that binds IL-6 with low affinity (IL-6 R alpha ) and a signal-transducing component of 130 kDa (gp130) that does not bind IL-6 by itself, but is required for high-affinity binding of IL-6 by the complex. Both components of the receptor complex, IL-6 R alpha and gp130 have been cloned, sequenced, and expressed (1-4).

A soluble form of the IL-6 R alpha has been found in the urine of healthy adult humans (5). This soluble receptor apparently arises from proteolytic cleavage of membrane-bound IL-6 R alpha and is about 50kDa in size. No naturally-occurring mRNA encoding a truncated form of the IL-6 R alpha has been reported. Soluble forms of human and murine IL-6 R alpha s have been constructed, however, by insertion of termination codons into the regions of the IL-6 R alpha cDNAs encoding the external portions of the receptors and prior to the transmembrane domains. These soluble receptors have been expressed in COS-7 and CHO cells and have been shown to bind to IL-6 in solution and to augment the activity of IL-6 as a result of the binding of the IL-6/IL-6 R alpha complex to membrane-bound gp130 (6, 7).

  • References:
    1. Yamasaki et al. (1988) Science 241:825.
    2. Baumann et al. (1990) J. Biol. Chem. 265:19853.
    3. Hibi et al. (1990) Cell 63:1149.
    4. Schooltink et al. (1991) Eur. J. Biochem. 277:659.
    5. Novick et al. (1989) J. Exp. Med. 170:1409.
    6. Yasukawa et al. (1990) J. Biochem. 108:673.
    7. Saito et al. (1991) J. Immunology 147:168.
  • Long Name:
    Interleukin 6 Receptor alpha
  • Entrez Gene IDs:
    3570 (Human); 16194 (Mouse)
  • Alternate Names:
    CD126; IL-6 R alpha; IL6R alpha; IL6R; IL-6Ra
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Lipopolysaccharide-squamous cell carcinoma-monocyte interactions induce cancer-supporting factors leading to rapid STAT3 activation.
    Authors: Kurago ZB, Lam-ubol A, Stetsenko A
    Head Neck Pathol, 2008;2(1):1-12.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
Isotype Controls
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Normal Goat IgG Control

Ctrl AB-108-C 191  
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Goat IgG (H+L) APC-conjugated Antibody

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Goat IgG Horseradish Peroxidase-conjugated Antibody

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Goat IgG VisUCyte HRP Polymer

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Goat IgG (H+L) Fluorescein-conjugated Antibody

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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Chicken Anti-Goat IgG Biotinylated Antibody

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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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