Human IL-9 DuoSet ELISA

Catalog # Availability Size / Price Qty
DY209-05
Ancillary Products Available
Human IL-9 ELISA Standard Curve
1 Image
Product Details
Citations (3)
FAQs
Supplemental Products
Reviews (1)

Human IL-9 DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Sufficient Materials
For five 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and Recombinant . The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required


PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Data Example

Human IL-9 ELISA Standard Curve

Product Datasheets

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-9

Mouse interleukin 9 (IL-9) was originally identified as T cell-derived T cell growth factor III/P40 that could support the long term growth of certain mouse T helper clones in the absence of antigen or antigen-presenting cells. Human IL-9 was independently cloned as a novel growth factor that is mitogenic for the human megakaryoblastic leukemic cell line, M07e. Human and mouse IL-9 share 56% identity at the amino acid level. Although mouse IL-9 is active on human cells, human IL-9 is not active on mouse cells.

Long Name:
Interleukin 9
Entrez Gene IDs:
3578 (Human); 16198 (Mouse)
Alternate Names:
Cytokine P40; HP40; IL9; IL-9; IL-9homolog of mouse T cell and mast cell growth factor 40; interleukin 9; interleukin-9; p40 cytokine; p40 T-cell and mast cell growth factor; P40; T-cell growth factor P40

Citations for Human IL-9 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. In Vitro Induction of T Helper 17 Cells by Synergistic Activation of Human Monocyte-Derived Langerhans Cell-Like Cells with Bacterial Agonists
    Authors: R Gramlich, E Aliahmadi, M Peiser
    Int J Mol Sci, 2019;20(6):.
    Species: Human
    Sample Types: Cell Culture Supernates
  2. Inflammatory Cytokines and T-Lymphocyte Subsets in Serum and Sputum in Patients with Bronchial Asthma and Chronic Obstructive Pulmonary Disease
    Authors: Y Bai, Q Zhou, Q Fang, L Song, K Chen
    Med. Sci. Monit., 2019;25(0):2206-2210.
    Species: Human
    Sample Types: Sputum
  3. Possible pathogenic role of T helper type 9 cells and interleukin (IL)-9 in atopic dermatitis.
    Authors: Ma L, Xue H, Guan X, Shu C, Zhang J, Yu J
    Clin Exp Immunol, 0;175(1):25-31.
    Species: Human
    Sample Types: Serum

FAQs

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Reviews for Human IL-9 DuoSet ELISA

Average Rating: 3 (Based on 1 Review)

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Human IL-9 DuoSet ELISA
By Anonymous on 04/02/2020
Application: Sample Tested: Serum and Plasma

We used this kit for the quantification of IL-9 in human serum and plasma. We could not detect IL-9 in our samples. Standard curve works very well and well-described protocol.