Human Indoleamine 2,3-dioxygenase/IDO Antibody
Human Indoleamine 2,3-dioxygenase/IDO Antibody Summary
Accession # P14902
This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human Indoleamine 2,3‑dioxygenase/IDO Monoclonal Antibody (Catalog # MAB10498).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Indoleamine 2,3-dioxygenase/IDO DuoSet ELISA Kit (Catalog # DY6030-05) for convenient development of a sandwich ELISA.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Human Indoleamine 2,3‑dioxygenase/IDO ELISA Standard Curve. Recombinant Human Indoleamine 2,3‑dioxygenase/IDO protein was serially diluted 2-fold and captured by Mouse Anti-Human Indoleamine 2,3‑dioxygenase/IDO Monoclonal Antibody (MAB10498) coated on a Clear Polystyrene Microplate (DY990). Mouse Anti-Human Indoleamine 2,3‑dioxygenase/IDO Monoclonal Antibody (Catalog # MAB104981) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994 ).
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Indoleamine 2,3-dioxygenase/IDO
Indoleamine 2,3-dioxygenase (IDO) is a heme-containing intracellular dioxygenase catalyzing the degradation of the essential amino acid L-tryptophan to N-formyl-kynurenine (1). This degradation is the first and rate-limiting step of the L-kynurenine pathway (2). IDO is widely expressed in dendritic cells, macrophages, microglia, eosinophils, fibroblasts, endothelial cells, and most tumor cells. In immune cells, its expression is mainly induced by cytokines such as IFN-gamma, IFN-alpha, IFN-beta, and IL‑10. IDO has an antimicrobial function due to its decreasing the availability of the essential amino acid tryptophan in inflammatory environments (3). Recent studies have demonstrated that IDO induces immunosuppression during infection, pregnancy, transplantation, autoimmunity, and neoplasia (3-5).
- Lewis-Ballester, A. et al. (2009) Proc. Natl. Acad. Sci. USA. 106:17371.
- Costantino, G. (2009) Expert Opin. Ther. Targets 13:247.
- Xu, H. et al. (2008) Immunol. Lett. 121:1.
- Lob, S. et al. (2009) Nat. Rev. Cancer 9:445.
- Curti, A. et al. (2009) Blood 113:2394.
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