Detects human Integrin alpha 2b/CD41 in direct ELISAs.
In direct ELISAs, no cross-reactivity
with recombinant human Integrin alpha 5, alpha 8, alpha V, beta 3, beta 5, beta 6, recombinant mouse Integrin beta 2b or beta 3 is observed.
Monoclonal Mouse IgG1 Clone # 745201
Protein A or G purified from hybridoma culture supernatant
heterodimer of human Integrin alpha 2B (Leu32-Arg993; R887L) Accession P08514 + human Integrin beta 3 (Gly27-Asp718) Accession P05106
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
2.5 µg/106 cells
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human Integrin alpha 2b/CD41 by Western Blot. Western blot shows lysates of human platelets. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Integrin alpha 2b/CD41 Monoclonal Antibody (Catalog # MAB7616) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Integrin alpha 2b/CD41 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 5.
Detection of Integrin alpha 2b/CD41 in Human Blood Platelets by Flow Cytometry. Human peripheral blood platelets were stained with Fluorescein-conjugated Anti-Mouse Integrin alpha 2b/CD41 (Clone P2) and either (A) Mouse IgG1 Isotype Control (Catalog # MAB002) or (B) Mouse Anti-Human Integrin alpha 2b/CD41 Monoclonal Antibody (Catalog # MAB7616) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). MAB7616 stains the same population as Clone P2.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin alpha 2b/CD41
Integrin alpha 2b (ITGA2b), also known as CD41 and GPIIb, is a transmembrane glycoprotein that is expressed by megakaryocytes and platelets. It is cleaved into two disulfide-linked chains (114 kDa and 22 kDa) during transit through the Golgi. Integrin alpha 2b associates with Integrin beta 3 to form complexes that interact with Fibrinogen, von Willebrand factor, Fibronectin, and Vitronectin. Integrin alpha 2b is required for platelet aggregation, and defects lead to disorders of coagulation. Within the extracellular domain, human Integrin alpha 2b shares 80% and 78% amino acid sequence identity with mouse and rat Integrin alpha 2b, respectively.
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