Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Chinese Hamster

Applications

Validated:

Western Blot, Flow Cytometry, CyTOF-ready

Cited:

Western Blot, ELISA Detection, Interferent Testing

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 745201
View all Integrin alpha 2b/CD41 Primary Antibodies »

Product Specifications

Immunogen

heterodimer of human Integrin alpha 2B (Leu32-Arg993; R887L) Accession P08514 + human Integrin beta 3 (Gly27-Asp718) Accession P05106

Specificity

Detects human Integrin alpha 2b/CD41 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Integrin alpha 5, alpha 8, alpha V, beta 3, beta 5, beta 6, recombinant mouse Integrin beta 2b or beta 3 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Integrin  alpha 2b/CD41 Antibody

Detection of Human Integrin a2b/CD41 antibody by Western Blot.

Detection of Human Integrin alpha 2b/CD41 by Western Blot.

Western blot shows lysates of human platelets. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Integrin a2b/CD41 Monoclonal Antibody (Catalog # MAB7616) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). A specific band was detected for Integrin a2b/CD41 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 5.

Detection of Integrin a2b/CD41 antibody in Human Blood Platelets antibody by Flow Cytometry.

Detection of Integrin alpha 2b/CD41 in Human Blood Platelets by Flow Cytometry.

Human peripheral blood platelets were stained with Fluorescein-conjugated Anti-Mouse Integrin a2b/CD41 (Clone P2) and either (A) Mouse IgG1Isotype Control (MAB002) or (B) Mouse Anti-Human Integrin a2b/CD41 Mono-clonal Antibody (Catalog # MAB7616) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). MAB7616 stains the same population as Clone P2.

Detection of Integrin alpha 2b/CD41 in Human Blood Platelets by Flow Cytometry.

Human peripheral blood platelets were stained with Mouse Anti-Human Integrin a2b/CD41 Mono-clonal Antibody (Catalog # MAB7616, filled histogram) or Mouse IgG1 Isotype Control (MAB002, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Integrin  alpha 2b/CD41 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Human peripheral blood platelets

Western Blot

2 µg/mL
Sample: Human platelets

Reviewed Applications

Read 1 review rated 5 using MAB7616 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Integrin alpha 2b/CD41

Integrin alpha 2b (ITGA2b), also known as CD41 and GPIIb, is a transmembrane glycoprotein that is expressed by megakaryocytes and platelets. It is cleaved into two disulfide-linked chains (114 kDa and 22 kDa) during transit through the Golgi. Integrin alpha 2b associates with Integrin beta 3 to form complexes that interact with Fibrinogen, von Willebrand factor, Fibronectin, and Vitronectin. Integrin alpha 2b is required for platelet aggregation, and defects lead to disorders of coagulation. Within the extracellular domain, human Integrin alpha 2b shares 80% and 78% amino acid sequence identity with mouse and rat Integrin alpha 2b, respectively.

Alternate Names

CD41, GP2B, GPIIb, GTA, HPA3, ITGA2b

Entrez Gene IDs

3674 (Human); 16399 (Mouse); 29350 (Rat); 102119413 (Cynomolgus Monkey)

Gene Symbol

ITGA2B

Additional Integrin alpha 2b/CD41 Products

Product Documents for Human Integrin  alpha 2b/CD41 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Integrin  alpha 2b/CD41 Antibody

For research use only

Citations for Human Integrin  alpha 2b/CD41 Antibody

Customer Reviews for Human Integrin  alpha 2b/CD41 Antibody (1)

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  • Human Integrin  alpha 2b/CD41 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 09/22/2019
    Human Integrin  alpha 2b/CD41 Antibody MAB7616

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