Detection of Human Intelectin‑2 by Western Blot. Western blot shows lysates of human small intestine tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Intelectin‑2 Monoclonal Antibody (Catalog # MAB8004) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Intelectin‑2 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Intelectin‑2 in Human Colon. Intelectin‑2 was detected in formalin fixed paraffin-embedded sections of human small intestine using Mouse Anti-Human Intelectin‑2 Monoclonal Antibody (Catalog # MAB8004) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to Paneth cells in intestinal glands. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Intelectin-2 (ITLN-2; also endothelial lectin 2 and HL-2) is a 37-41 kDa, likely Ca-dependent carbohydrate-binding lectin that is a member of the X-lectin family of molecules. It is secreted by intestinal Paneth cells, and appears to participate in fungal microbe recognition. The human ITLN-2 precursor is 325 amino acids (aa) in length (SwissProt #:Q8WWU7). It contains a 26 aa signal sequence and a 299 aa mature region. Although mouse ITLN-2 is reportedly GPI-linked, and mouse and human ITLN-2 show considerable C-terminal aa identity, it is not known if human ITNL-2 is also GPI-linked. There is one fibrinogen-related domain in the mature molecule (aa 44-267). One potential splice variant is reported that shows a premature truncation after Gly264. Over aa 29-324, human ITLN-2 shares 79% aa sequence identity with mouse ITLN-2/ITLN-1b, and 85% aa sequence identity with human ITLN-1.
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