Human IRF1 Antibody

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Detection of Human IRF1 by Western Blot.
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Product Details
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Human IRF1 Antibody Summary

Species Reactivity
Detects human IRF1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) IRF2 (aa 151-349), rhIRF3 (aa 206-427), rhIRF4 (aa 130-451), rhIRF5 (aa 214-395), rhIRF6 (aa 176-366), rhIRF7 (aa 289-503), rhIRF8 (aa 255‑426), rhIRF9 (aa 238-393), or recombinant mouse IRF1 (aa 147-329) is observed.
Monoclonal Mouse IgG2B Clone # 686703
IgM-specific Affinity-purified from hybridoma culture supernatant
E. coli-derived recombinant human IRF1
Accession # P10914
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human IRF1 antibody by Western Blot. View Larger

Detection of Human IRF1 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line, MOLT-4 human acute lymphoblastic leukemia cell line, and Jurkat human acute T cell leukemia cell line. PVDF Membrane was probed with 0.5 µg/mL of Human IRF1 Monoclonal Antibody (Catalog # MAB4830) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IRF1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IRF1

IRF1 (interferon regulatory factor 1) is a 45 kDa member of the IRF family of proteins. It is induced by TLR9 and INF-gamma stimulation, and positively regulates the expression of IFN-beta, iNOS and 1L-12 p35.  Human IRF1 is 325 amino acids (aa) in length. It contains an N-terminal DNA-binding basic region (aa 1-140) and an acidic C-terminus (aa 141-325). Lys275 and 299 can be SUMOylated, which blocks activity, or ubiquinated at Lys299, which initiates degradation. There is a splice variant that shows an 11 aa substitution for the C-terminal 86 amino acids. Over aa 171-325, human IRF1 shares 83% aa identity with both canine and porcine IRF1.   

Long Name
Interferon Regulatory Factor 1
Entrez Gene IDs
3659 (Human); 16362 (Mouse); 24508 (Rat)
Alternate Names
interferon regulatory factor 1; IRF1; MAR; MARIRF-1

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