Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 686703
View all IRF1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human IRF1
Thr147-Pro325
Accession # P10914

Specificity

Detects human IRF1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) IRF2 (aa 151-349), rhIRF3 (aa 206-427), rhIRF4 (aa 130-451), rhIRF5 (aa 214-395), rhIRF6 (aa 176-366), rhIRF7 (aa 289-503), rhIRF8 (aa 255‑426), rhIRF9 (aa 238-393), or recombinant mouse IRF1 (aa 147-329) is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human IRF1 Antibody

Detection of Human IRF1 antibody by Western Blot.

Detection of Human IRF1 by Western Blot.

Western blot shows lysates of Raji human Burkitt's lymphoma cell line, MOLT-4 human acute lymphoblastic leukemia cell line, and Jurkat human acute T cell leukemia cell line. PVDF Membrane was probed with 0.5 µg/mL of Human IRF1 Monoclonal Antibody (Catalog # MAB4830) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IRF1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human IRF1 Antibody

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: Raji human Burkitt's lymphoma cell line, MOLT‑4 human acute lymphoblastic leukemia cell line, and Jurkat human acute T cell leukemia cell line

Formulation, Preparation, and Storage

Purification

IgM-specific Affinity-purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: IRF1

IRF1 (interferon regulatory factor 1) is a 45 kDa member of the IRF family of proteins. It is induced by TLR9 and INF-gamma stimulation, and positively regulates the expression of IFN-beta, iNOS and 1L-12 p35.  Human IRF1 is 325 amino acids (aa) in length. It contains an N-terminal DNA-binding basic region (aa 1-140) and an acidic C-terminus (aa 141-325). Lys275 and 299 can be SUMOylated, which blocks activity, or ubiquinated at Lys299, which initiates degradation. There is a splice variant that shows an 11 aa substitution for the C-terminal 86 amino acids. Over aa 171-325, human IRF1 shares 83% aa identity with both canine and porcine IRF1.   

Long Name

Interferon Regulatory Factor 1

Alternate Names

MAR

Entrez Gene IDs

3659 (Human); 16362 (Mouse); 24508 (Rat)

Gene Symbol

IRF1

UniProt

P10914

Additional IRF1 Products

Product Documents for Human IRF1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human IRF1 Antibody

For research use only

Customer Reviews for Human IRF1 Antibody

There are currently no reviews for this product. Be the first to review Human IRF1 Antibody and earn rewards!

Have you used Human IRF1 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies