Human ITFG1 Antibody
Human ITFG1 Antibody Summary
Accession # Q8TB96
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human ITFG1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, human brain (cerebellum) tissue, and human testis tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ITFG1 Monoclonal Antibody (Catalog # MAB8900) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for ITFG1 at approximately 95-100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Integrin-alpha FG-GAP repeat-containing protein 1 (ITFG1), also known as T cell immunomodulatory protein (TIP), was initially identified using bioinformatics and high-throughput cell-based screening assays to isolate novel factors involved in T cell biology. A ubiquitously expressed 98 kDa glycoprotein, ITFG1 contains an N-terminal signal peptide and a C-terminal transmembrane domain flanking twelve potential N-linked glycosylation sites. Human and mouse T cells treated with ITFG1 in vitro secreted the cytokines IFN-g, TNF-a and IL-10, while in vivo ITFG1 was protective in a mouse acute graft-versus-host disease (GVHD) model. Over amino acids (aa) 1-566, human ITFG1 shares 89% and 88% aa identity with mouse and rat ITFG1, respectively.
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