Key Product Details

Species Reactivity

Human

Applications

Western Blot, Neutralization, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 341419
View all Kallikrein 13 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Kallikrein 13
Val19-Ile262
Accession # Q9UKR3

Specificity

Detects human Kallikrein 13 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Kallikrein 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 14, 15, rhKallikrein B1, rhFactorVII, rhHGFA, rhUPA, rhThrombin, rhFactor X, or rhFactor XI is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications for Human Kallikrein 13 Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Kallikrein 13 (Catalog # 2625-SE), see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Recombinant Human Kallikrein 13 (Catalog # 2625-SE)

Neutralization

Measured by its ability to neutralize Recombinant Human Kallikrein 13 (1 µg/mL, Catalog # 2625-SE) cleavage of the fluorogenic peptide substrate Boc-VPR-AMC (100 µM, Catalog # ES011). The Neutralization Dose (ND50) is typically 2.4 µg/mL.

Reviewed Applications

Read 1 review rated 5 using MAB2625 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Kallikrein 13

Human tissue Kallikrein 13 (hK13) is a member of the human tissue kallikrein family. The gene encoding for rhK13 is termed KLK13 and it resides on chromosome 19q13.3-4 along with fourteen other members of the family. KLK13 spans about 8.7 kb genomic DNA and the longest transcript is 831 bp, encoding for a protein of 277 amino acids (1). Another five shorter splice variants have also been identified. They are specifically expressed in the testicular tissue and encode for five truncated forms of hK13 (2). Due to the aspartic acid residue in the substrate binding pocket, the enzymatic activity of hK13 is predicted be trypsin-like. It has been shown that recombinant hK13 produced in yeast can cleave synthetic peptides after the arginine residue and some extracellular matrix components (3). However, its exact physiological substrates and functions remain obscure. Despite the lack of knowledge on the physiological function of hK13, several studies have demonstrated that hK13 is implicated with cancer of the breast and ovary and it can serve as a favorable prognostic biomarker for these malignancies (4, 5). The 277 amino acid hKLK13 precursor consists of a signal peptide (residues 1 to 18), a pro peptide (residues 19 to 25) and an active protein (residues 26 to 277). The amino acid sequence of hKLK13 is 84%, 81%, and 80% identical to that of canine, mouse and rat.

References

  1. Yousef, G.M. et al. (2000) J. Biol. Chem. 275:11891.
  2. Chang, A. et al. (2001) Anticancer Res. 21:3147.
  3. Kapadia, C. et al. (2004) Biochem. Biophys. Res. Commun. 323:1084.
  4. Chang, A. et al. (2002) Br. J. Cancer. 86:1457.
  5. Scorilas, A. et al. (2004) J. Clin. Oncol. 22:678.

Alternate Names

KLK13

Entrez Gene IDs

26085 (Human)

Gene Symbol

KLK13

UniProt

Q9UKR3

Additional Kallikrein 13 Products

Product Documents for Human Kallikrein 13 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Kallikrein 13 Antibody

For research use only

Citations for Human Kallikrein 13 Antibody

Customer Reviews for Human Kallikrein 13 Antibody (1)

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  • Human Kallikrein 13 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 07/05/2022
    Human Kallikrein 13 Antibody MAB2625

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