Human Laminin  alpha 1 N-Terminus Domain VI Antibody

Detection of Laminin alpha 1 in U2OS Human Cell Line by Flow Cytometry.

U2OS human osteosarcoma cell line was stained with Goat Anti-Human Laminin alpha 1 Antigen Affinity-Purified Polyclonal Antibody (Catalog # AF4187, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by APC-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Detection of Laminin alpha 1 N-Terminus Domain VI by Western Blot

Extracellular matrix (ECM) deposition in fetal bovine serum (FBS) or human platelet lysate (HPL)-cultured adipose-derived stem cell (ASC) sheets. (A) Gene expression of collagen 1 alpha 1 (COL1A1), fibronectin (FN1), and laminin (LAMA1) in ASCs under different culture condition (n = 3). (B) Sircol assay of ASCs cultured under different culture condition (n = 3). (C) Western blot analysis of the expression of the ECM proteins in FBS or HPL-cultured ASC sheets. (D) Venn diagram of the number of proteins detected on LC/MS in the ECM of ASC sheets cultured with FBS and HPL, respectively (*p < 0.05; **p < 0.01; ***p < 0.005 from control, #p < 0.05; ##p < 0.01; ###p < 0.005 between the indicated groups). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33195191), licensed under a CC-BY license. Not internally tested by R&D Systems.