Human Langerin/CD207 Antibody Summary
Accession # Q9UJ71
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Langerin/CD207 in Human Skin. Langerin/CD207 was detected in immersion fixed paraffin-embedded sections of human skin using 15 µg/mL Goat Anti-Human Langerin/CD207 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2088) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of Langerhans cells in the skin epidermis. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Langerin (also known as CD207) is a type II transmembrane glycoprotein which is member K of the C-type lectin domain family 4 (1). Langerin is used as a marker for Langerhans cells (LCs) which represent the immature dendritic cells in the epidermis (1, 2). LCs uniquely contain “tennis racket”-shaped endosomal recycling compartment subdomains with pentalamellar membranes termed Birbeck granules (1-3). Langerin is necessary and sufficient for Birbeck granule formation (1). The 328 amino acid (aa) human langerin sequence contains a 43 aa cytoplasmic domain, a 21 aa transmembrane domain and a 264 aa extracellular domain (ECD) that contains a coiled-coil domain and a single C-type lectin domain. Trimerization greatly increases the lectin binding affinity (4). Langerin internalizes endogenous proteins such as type I procollagen. Internalization by LC is thought to lead to suppression of self reactions (4-6). Langerin also mediates endocytosis of non-peptide antigens containing mannose, N-acetyl glucosamine and fucose that are expressed by mycobacteria and fungae (4, 7). Some antigens, such as the M. leprae glycolipid arabinomycolate, are ultimately presented by human LC CD1a in cutaneous-draining lymph nodes (8). Langerin performs a barrier-like function to HIV-1 transmission due to its internalization of virus particles for destruction (9). A rare human polymorphism within the lectin domain, W264R, abolishes both carbohydrate recognition and Birbeck granule formation (10, 11). Genetic deletion of mouse langerin was not shown to have functional consequence other than abolishing Birbeck granule formation (12). Human langerin shares 68%, 62%, 71% aa identity with mouse, rat, and bovine langerin ECD, respectively.
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- Stambach, N.S. and M.E. Taylor (2003) Glycobiology 13:401.
- Tada, Y. et al. (2006) J. Invest. Dermatol. 126:1549.
- Ritter, U. and A. Osterloh (2007) Med. Microbiol. Immunol. 196:51.
- Takahara, K. et al. (2003) Int. Immunol. 16:819.
- Hunger, R.E. et al. (2004) J. Clin. Invest. 113:701.
- De Witte, L. et al. (2007) Nat. Med. 13:367.
- Verdijk, P. et al. (2005) J. Invest. Dermatol. 124:714.
- Ward, E.M. et al. (2006) J. Biol. Chem. 281:15450.
- Kissenpfennig, A. et al. (2005) Mol. Cell. Biol. 25:88.
Citations for Human Langerin/CD207 Antibody
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Citations: Showing 1 - 4
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Chemokine Levels in the Penile Coronal Sulcus Correlate with HIV-1 Acquisition and Are Reduced by Male Circumcision in Rakai, Uganda
PLoS Pathog, 2016;12(11):e1006025.
Sample Types: Whole Tissue
HIV-1 replication in Langerhans and interstitial dendritic cells is inhibited by neutralizing and Fc-mediated inhibitory antibodies.
Authors: Peressin M, Holl V, Schmidt S, Decoville T, Mirisky D, Lederle A, Delaporte M, Xu K, Aubertin AM, Moog C
J. Virol., 2011;85(2):1077-85.
Sample Types: Whole Cells
Plasmacytoid dendritic cells infiltrate the skin in positive tuberculin skin test indurations.
Authors: Bond E, Liang F, Sandgren KJ, Smed-Sorensen A, Bergman P, Brighenti S, Adams WC, Betemariam SA, Rangaka MX, Lange C, Wilkinson RJ, Andersson J, Lore K
J. Invest. Dermatol., 2011;132(1):114-23.
Sample Types: Whole Tissue
In situ distribution of HIV-binding CCR5 and C-type lectin receptors in the human endocervical mucosa.
Authors: Hirbod T, Kaldensjo T, Broliden K
PLoS ONE, 2011;6(9):e25551.
Sample Types: Whole Tissue
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