Detection of Human LAT by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line and MOLT‑4 human acute lymphoblastic leukemia cell line. PVDF Membrane was probed with 0.1 µg/mL of Human LAT Monoclonal Antibody (Catalog # MAB63341) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for LAT at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of LAT in Human PBMCs by Flow Cytometry.
Human peripheral blood lymphocytes were stained with Human LAT Monoclonal Antibody (Catalog # MAB63341) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B) and Human CD3 epsilon PE-conjugated Monoclonal Antibody (Catalog # FAB100P). Quadrant markers were set based on control antibody staining (Catalog # MAB0041).
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
LAT (Linker for activation of T cells; also pp36) is a 36-38 kDa type III (no signal sequence; extracellular N-terminus) transmembrane adaptor phosphoprotein. It is expressed on mast cells, NK cells, monocytes (but not macrophages), platelets and T cells. LAT is phosphorylated upon ligation of the TCR, GPVI, and Fc epsilon RI, and serves as an anchor for several SH2-domain containing signaling proteins, including PLCg1, SLP76 and Grb2. Human LAT is 262 amino acids (aa) in length. It contains a four aa extracellular segment with a 235 aa cytoplasmic domain (aa 28-262). Palmitoylation occurs on Cys26 and 29. There are 13 Ser/Thr phosphorylation sites plus five Tyr sites. Multiple potential splice variants are reported. There is an alternative start site 36 aa upstream of the standard site that may be accompanied by a deletion of aa 114-142. There is also a deletion of aa 88-97, and three substitutions: two aa for aa 215-262, 38 aa for aa 55-262, and 50 aa for aa 35-262. Over aa 30-113, human LAT shares 73% aa identity with mouse LAT.
Linker for Activation of T cells
Entrez Gene IDs:
27040 (Human); 16797 (Mouse); 81511 (Rat)
36 kDa phospho-tyrosine adapter protein; LAT; LAT1,36 kDa phospho-tyrosine adaptor protein; linker for activation of T cells; linker for activation of T-cells family member 1; p36-38; pp36
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