Human LAT Antibody

Catalog # Availability Size / Price Qty
MAB63341
MAB63341-SP
Detection of Human LAT by Western Blot.
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Product Details
Citations (1)
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Human LAT Antibody Summary

Species Reactivity
Human
Specificity
Detects human LAT in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 661002
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human LAT
His30-Gln113
Accession # O43561
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human LAT antibody by Western Blot. View Larger

Detection of Human LAT by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and MOLT-4 human acute lymphoblastic leukemia cell line. PVDF Membrane was probed with 0.1 µg/mL of Human LAT Monoclonal Antibody (Catalog # MAB63341) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for LAT at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of LAT antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of LAT in Human PBMCs by Flow Cytometry. Human peripheral blood lymphocytes were stained with Human LAT Monoclonal Antibody (Catalog # MAB63341) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B) and Human CD3e PE-conjugated Monoclonal Antibody (Catalog # FAB100P). Quadrant markers were set based on control antibody staining (Catalog # MAB0041).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LAT

LAT (Linker for activation of T cells; also pp36) is a 36-38 kDa type III (no signal sequence; extracellular N-terminus) transmembrane adaptor phosphoprotein. It is expressed on mast cells, NK cells, monocytes (but not macrophages), platelets and T cells. LAT is phosphorylated upon ligation of the TCR, GPVI, and Fc epsilon RI, and serves as an anchor for several SH2-domain containing signaling proteins, including PLCg1, SLP76 and Grb2. Human LAT is 262 amino acids (aa) in length. It contains a four aa extracellular segment with a 235 aa cytoplasmic domain (aa 28-262). Palmitoylation occurs on Cys26 and 29. There are 13 Ser/Thr phosphorylation sites plus five Tyr sites. Multiple potential splice variants are reported. There is an alternative start site 36 aa upstream of the standard site that may be accompanied by a deletion of aa 114-142. There is also a deletion of aa 88-97, and three substitutions: two aa for aa 215-262, 38 aa for aa 55-262, and 50 aa for aa 35-262. Over aa 30-113, human LAT shares 73% aa identity with mouse LAT.  

Long Name
Linker for Activation of T cells
Entrez Gene IDs
27040 (Human); 16797 (Mouse); 81511 (Rat)
Alternate Names
36 kDa phospho-tyrosine adapter protein; LAT; LAT1,36 kDa phospho-tyrosine adaptor protein; linker for activation of T cells; linker for activation of T-cells family member 1; p36-38; pp36

Product Datasheets

Citation for Human LAT Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Rab6-dependent retrograde traffic of LAT controls immune synapse formation and T cell activation
    Authors: JM Carpier, AE Zucchetti, L Bataille, S Dogniaux, M Shafaq-Zad, S Bardin, M Lucchino, M Maurin, LD Joannas, JG Magalhaes, L Johannes, T Galli, B Goud, C Hivroz
    J. Exp. Med., 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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