Human Latrophilin 2/LPHN2 Antibody

R&D Systems | Catalog # MAB105881

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Flow Cytometry, CyTOF-ready

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 1030202
View all Latrophilin 2/LPHN2 Primary Antibodies »

Product Specifications

Immunogen

Human Latrophilin 2/LPHN2 synthetic peptide
Accession # O95490

Specificity

Detects human Latrophilin 2/LPHN2 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Latrophilin 2/LPHN2 Antibody

Detection of Human Latrophilin 2/LPHN2 by Western Blot.

Western blot shows lysates of HEK293T human embryonic kidney cell line either mock transfected or transfected with human Latrophilin 2/LPHN2. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Latrophilin 2/LPHN2 Monoclonal Antibody (Catalog # MAB105881) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Latrophilin 2/LPHN2 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of LPHN2 in HEK293 Human Cell Line transfected with Human LPHN2 by Flow Cytometry.

HEK293 Human Cell Line transfected with Human LPHN2 was stained with Mouse Anti-Human LPHN2 Monoclonal Antibody (Catalog # MAB105881, filled histogram) or isotype control antibody (MAB10041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our Staining Intracellular Molecules protocol.

Applications for Human Latrophilin 2/LPHN2 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: HEK293 Human Cell Line transfected with Human LPHN2

Western Blot

1 µg/mL
Sample: HEK293T human embryonic kidney cell line transfected with human Latrophilin 2/LPHN2

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Latrophilin 2/LPHN2

Latrophilin 2 (LPHN2) is a G-protein coupled receptor which functions in both cell adhesion and signal transduction. Latrophilin represents a phylogenetically conserved family of receptors which has an unusual structure consisting of two fragments that are produced by proteolytic cleavage of the parental molecule and behave independently in the plasma membrane. LPHN2 is a ubiquitous receptor for alpha-latroxin which is a protein toxin in black widow spider venom that is toxic to vertebrates.

Long Name

Latrophilin 2

Alternate Names

ADGRL2, CIRL2, LEC1, LPHH1, LPHN2

Entrez Gene IDs

23266 (Human)

Gene Symbol

ADGRL2

UniProt

O95490

Additional Latrophilin 2/LPHN2 Products

Product Documents for Human Latrophilin 2/LPHN2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Latrophilin 2/LPHN2 Antibody

For research use only

Citations for Human Latrophilin 2/LPHN2 Antibody

Customer Reviews for Human Latrophilin 2/LPHN2 Antibody

There are currently no reviews for this product. Be the first to review Human Latrophilin 2/LPHN2 Antibody and earn rewards!

Have you used Human Latrophilin 2/LPHN2 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies