Human LILRA2/CD85h/ILT1 Antibody Summary
Accession # Q8N149
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human LILRA2/CD85h/ILT1 by Western Blot. Western blot shows lysates of human peripheral blood mononuclear cells (PBMC). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human LILRA2/CD85h/ILT1 Monoclonal Antibody (Catalog # MAB63641) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for LILRA2/CD85h/ILT1 at approximately 69 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Immune-like transcript 1 (ILT1), also known as leukocyte immunoglobulin-like receptor 7 (LIR-7), leukocyte immunoglobulin-like receptor subfamily A member 2 (LILRA2), and CD85H, is a 51 kDa (unglycosylated) type I transmembrane glycoprotein and member of the leukocyte immunoglobulin-like receptor family. Human ILT1 is synthesized as a 483 amino acid (aa) precursor that contains a 23 aa signal sequence, a 426 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 13 aa cytoplasmic region. The ECD contains four Ig-like C2-type domains and seven potential sites for N-linked glycosylation. A splice variant produces a second isoform that has a one aa substitution for aa 419-436 in the longer form. There are no murine orthologs for human ILT1. ILT1 may act as a receptor for class I MHC antigens.
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