Human LILRA2/CD85h/ILT1 Antibody

Catalog # Availability Size / Price Qty
MAB63641-100
MAB63641-SP
Human LILRA2/CD85h/ILT1 Antibody in Western Blot
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Human LILRA2/CD85h/ILT1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human LILRA-2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) LILRA-1, rhLILRA-3, rhLILRA-4, rhLILRA-5, rhLILRA-6, rhILT-2, rhILT-4, or rhILT-5 is observed.
Source
Monoclonal Mouse IgG2B Clone # 600002
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human LILRA2/CD85h/ILT1
Pro17-Ser437
Accession # Q8N149
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human LILRA2/CD85h/ILT1 by Western Blot. View Larger

Detection of Human LILRA2/CD85h/ILT1 by Western Blot. Western blot shows lysates of human peripheral blood mononuclear cells (PBMC). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human LILRA2/CD85h/ILT1 Monoclonal Antibody (Catalog # MAB63641) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for LILRA2/CD85h/ILT1 at approximately 69 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LILRA2/CD85h/ILT1

Immune-like transcript 1 (ILT1), also known as leukocyte immunoglobulin-like receptor 7 (LIR-7), leukocyte immunoglobulin-like receptor subfamily A member 2 (LILRA2), and CD85H, is a 51 kDa (unglycosylated) type I transmembrane glycoprotein and member of the leukocyte immunoglobulin-like receptor family. Human ILT1 is synthesized as a 483 amino acid (aa) precursor that contains a 23 aa signal sequence, a 426 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 13 aa cytoplasmic region. The ECD contains four Ig-like C2-type domains and seven potential sites for N-linked glycosylation. A splice variant produces a second isoform that has a one aa substitution for aa 419-436 in the longer form. There are no murine orthologs for human ILT1. ILT1 may act as a receptor for class I MHC antigens.

Long Name
Leukocyte Immunoglobulin-like Receptor, Subfamily A (with TM domain), Member 2
Entrez Gene IDs
11027 (Human)
Alternate Names
CD85 antigen-like family member H; CD85h antigen; CD85h; ILT1; ILT-1; ILT1CD85H; Leukocyte immunoglobulin-like receptor 7; leukocyte immunoglobulin-like receptor subfamily A member 2 soluble; leukocyte immunoglobulin-like receptor subfamily A member 2; leukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 2; LILRA2; LIR7; LIR7LIR-7Immunoglobulin-like transcript 1

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FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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