Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, Flow Cytometry, Immunocytochemistry, CyTOF-ready

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2381D
View all LRTM1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide containing human LRTM1
Accession # Q9HBL6

Specificity

Detects human LRTM1 in direct ELISAs.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human LRTM1 Antibody

Detection of Human LRTM1 antibody by Western Blot.

Detection of Human LRTM1 by Western Blot.

Western blot shows lysates of HEK293T human embryonic kidney cell line either mock transfected or transfected with human LRTM1 and eGFP. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human LRTM1 Monoclonal Antibody (Catalog # MAB10046) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for LRTM1 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of LRTM1 antibody in HEK293 Human Cell Line Transfected with Human LRTM1 and eGFP antibody by Flow Cytometry.

Detection of LRTM1 in HEK293 Human Cell Line Transfected with Human LRTM1 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with (A) human LRTM1 or (B) irrelevant transfectants and eGFP was stained with Rabbit Anti-Human LRTM1 Monoclonal Antibody (Catalog # MAB10046) followed by APC-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). Quadrant markers were set based on control antibody staining (Catalog # MAB1050). View our protocol for Staining Membrane-associated Proteins.
LRTM1 antibody in TK-6 and A549 Human Cell Lines by Immunocytochemistry (ICC).

LRTM1 in TK-6 and A549 Human Cell Lines.

LRTM1 was detected in immersion fixed TK-6 human lymphoblastoid cell line (left panel; positive stain) and A549 human lung carcinoma cell line (right panel; negative stain) using Rabbit Anti-Human LRTM1 Monoclonal Antibody (Catalog # MAB10046) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
LRTM1 antibody in Human Spleen by Immunohistochemistry (IHC-P).

LRTM1 in Human Spleen.

LRTM1 was detected in immersion fixed paraffin-embedded sections of human spleen using Rabbit Anti-Human LRTM1 Monoclonal Antibody (Catalog # MAB10046) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human LRTM1 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: HEK293 Human Cell Line Transfected with Human LRTM1 and EGFP

Immunocytochemistry

3-25 µg/mL
Sample: Immersion fixed TK-6 human lymphoblastoid cell line

Immunohistochemistry

3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human spleen

Western Blot

2 µg/mL
Sample: HEK293T human embryonic kidney cell line transfected with human LRTM1 and eGFP

Reviewed Applications

Read 1 review rated 5 using MAB10046 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LRTM1

LRTM1 belongs to the extracellular LRRs superfamily.  LRTM1 is involved in positive regulation of synapse assembly and is an integral component of the membrane.  LRTM1 has the highest expression level in pineal body.

Long Name

Leucine-rich Repeats and Transmembrane Domains 1

Alternate Names

HT017, leucine-rich repeat and transmembrane domain-containing protein 1, leucine-rich repeats and transmembrane domains 1

Entrez Gene IDs

57408 (Human); 319476 (Mouse)

Gene Symbol

LRTM1

UniProt

Q9HBL6

Additional LRTM1 Products

Product Documents for Human LRTM1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human LRTM1 Antibody

For research use only

Related Research Areas

Neuronal Lineage Markers

Customer Reviews for Human LRTM1 Antibody (1)

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  • Human LRTM1 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Spleen tissue
    Species: Human
    Verified Customer | Posted 12/31/2021
    Human LRTM1 Antibody MAB10046

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Protocols

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