Human Lysine Hydroxylase 2/PLOD2 Antibody (408105)

R&D Systems | Catalog # MAB4445

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 408105
View all Lysine Hydroxylase 2/PLOD2 Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Lysine Hydroxylase 2/PLOD2
Ala26-Pro758
Accession # NP_891988

Specificity

Detects human Lysine Hydroxylase 2/PLOD2 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Lysine Hydroxylase 2/PLOD2 Antibody (408105)

Detection of Lysine Hydroxylase 2/PLOD2 by Western Blot

Detection of Lysine Hydroxylase 2/PLOD2 by Western Blot

Chronic hypoxia affects metabolism in luminal breast cancer cells. A, B Heatmap of genes involved in glycolysis (A) and cytoskeleton (B) pathways in 24 h (acute) and 5 days (chronic) hypoxia in MCF-7 and HCC1143 cells. NA, not available; ****padj < 0.0001; ***padj < 0.001; **padj < 0.01; *padj < 0.05; ns, not significant. White color means that genes were filtered out in targeted RNA sequencing data after filtering by DESeq2 package in R software. C Lactate levels (mM) in 6 cell lines under normoxia normalized to the OD value determined in SRB assay. D, E Lactate levels measured in three luminal (MCF-7, T47D, BT474) (D) and 3 basal A (HCC1143, SUM149PT, HCC1806) (E) breast cancer cell lines under acute normoxia/ hypoxia and chronic normoxia/ hypoxia normalized to the OD value determined in SRB assay. F CA9 RNA expression level under hypoxia in luminal and basal A cell lines detected by qRT-PCR. Log2(2^(-delta delta CT)) was calculated by normalizing to normoxia in each cell line. Error bars indicate SD for triplicate measurements. ***p < 0.001; **p < 0.01; *p < 0.05. G GAPDH and PLOD2 protein expression detected by Western blot. B-actin serves as loading control. H Quantification of GADPH signal normalized to B-actin with Image J. Error bars indicate SD for triplicate measurements. **p < 0.01; *p < 0.05; ns, not significant Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36826702), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Lysine Hydroxylase 2/PLOD2 by Western Blot

Detection of Lysine Hydroxylase 2/PLOD2 by Western Blot

Chronic hypoxia affects metabolism in luminal breast cancer cells. A, B Heatmap of genes involved in glycolysis (A) and cytoskeleton (B) pathways in 24 h (acute) and 5 days (chronic) hypoxia in MCF-7 and HCC1143 cells. NA, not available; ****padj < 0.0001; ***padj < 0.001; **padj < 0.01; *padj < 0.05; ns, not significant. White color means that genes were filtered out in targeted RNA sequencing data after filtering by DESeq2 package in R software. C Lactate levels (mM) in 6 cell lines under normoxia normalized to the OD value determined in SRB assay. D, E Lactate levels measured in three luminal (MCF-7, T47D, BT474) (D) and 3 basal A (HCC1143, SUM149PT, HCC1806) (E) breast cancer cell lines under acute normoxia/ hypoxia and chronic normoxia/ hypoxia normalized to the OD value determined in SRB assay. F CA9 RNA expression level under hypoxia in luminal and basal A cell lines detected by qRT-PCR. Log2(2^(-delta delta CT)) was calculated by normalizing to normoxia in each cell line. Error bars indicate SD for triplicate measurements. ***p < 0.001; **p < 0.01; *p < 0.05. G GAPDH and PLOD2 protein expression detected by Western blot. B-actin serves as loading control. H Quantification of GADPH signal normalized to B-actin with Image J. Error bars indicate SD for triplicate measurements. **p < 0.01; *p < 0.05; ns, not significant Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36826702), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Lysine Hydroxylase 2/PLOD2 Antibody (408105)

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Lysine Hydroxylase 2, see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Recombinant Human Lysine Hydroxylase 2

Reviewed Applications

Read 1 review rated 4 using MAB4445 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Lysine Hydroxylase 2/PLOD2

Lysine Hydroxylase 2 (LH2) also known as PLOD2 (procollagen-lysine, 2 oxoglutarate 5 dioxygenase 2) is a membrane-bound homodimeric enzyme localized to the cisternae of the rough ER. The enzyme catalyzes the hydroxylation of lysine residues in collagen-like peptides, which in turn serves as sites for carbohydrate attachment in collagen. Two alternatively spliced forms have 737 or 758 amino acids (aa). Human LH2 (758 aa form) shares 92.5% aa sequence identity with the appropriate isoform of mouse and rat LH2.

Alternate Names

LH2, PLOD2, TLH

Entrez Gene IDs

5352 (Human); 26432 (Mouse); 300901 (Rat)

Gene Symbol

PLOD2

UniProt

NP_891988

Additional Lysine Hydroxylase 2/PLOD2 Products

Product Documents for Human Lysine Hydroxylase 2/PLOD2 Antibody (408105)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Lysine Hydroxylase 2/PLOD2 Antibody (408105)

For research use only

Related Research Areas

Redox Enzymes

Citations for Human Lysine Hydroxylase 2/PLOD2 Antibody (408105)

Customer Reviews for Human Lysine Hydroxylase 2/PLOD2 Antibody (408105) (1)

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  • Human Lysine Hydroxylase 2/PLOD2 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: MDA-MB-231 human breast cancer cell line
    Species: Human
    Verified Customer | Posted 05/02/2016
    western blot detection of PLOD2 in MDA-MB-231 transfected with siRNA against PLOD2. We routinely detect PLOD2 around 100KDa,this is likely related to post-translation modifications of PLOD2
    Human Lysine Hydroxylase 2/PLOD2 Antibody (408105) MAB4445

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