Human Lysine Hydroxylase 2/PLOD2 Antibody

Catalog # Availability Size / Price Qty
MAB4445
MAB4445-SP
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews (1)

Human Lysine Hydroxylase 2/PLOD2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Lysine Hydroxylase 2/PLOD2 in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG2B Clone # 408105
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Lysine Hydroxylase 2/PLOD2
Ala26-Pro758
Accession # NP_891988
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Lysine Hydroxylase 2
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Lysine Hydroxylase 2, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lysine Hydroxylase 2/PLOD2

Lysine Hydroxylase 2 (LH2) also known as PLOD2 (procollagen-lysine, 2 oxoglutarate 5 dioxygenase 2) is a membrane-bound homodimeric enzyme localized to the cisternae of the rough ER. The enzyme catalyzes the hydroxylation of lysine residues in collagen-like peptides, which in turn serves as sites for carbohydrate attachment in collagen. Two alternatively spliced forms have 737 or 758 amino acids (aa). Human LH2 (758 aa form) shares 92.5% aa sequence identity with the appropriate isoform of mouse and rat LH2.

Entrez Gene IDs
5352 (Human); 26432 (Mouse); 300901 (Rat)
Alternate Names
2-oxoglutarate 5-dioxygenase (lysine hydroxylase) 2; 2-oxoglutarate 5-dioxygenase 2; LH2; Lysine Hydroxylase 2; PLOD2; procollagen-lysine; telopeptide lysyl hydroxylase; TLH

Product Datasheets

Citations for Human Lysine Hydroxylase 2/PLOD2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by:

  1. Lysyl Hydroxylase 2 Is Secreted By Tumor Cells and Can Modify Collagen in the Extracellular Space
    Authors: Jonathan Kurie
    J. Biol. Chem., 2016;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  2. Lysyl hydroxylase 2 induces a collagen cross-link switch in tumor stroma.
    Authors: Chen, Yulong, Terajima, Masahiko, Yang, Yanan, Sun, Li, Ahn, Young-Ho, Pankova, Daniela, Puperi, Daniel S, Watanabe, Takeshi, Kim, Min P, Blackmon, Shanda H, Rodriguez, Jaime, Liu, Hui, Behrens, Carmen, Wistuba, Ignacio, Minelli, Rosalba, Scott, Kenneth, Sanchez-Adams, Johannah, Guilak, Farshid, Pati, Debanand, Thilaganathan, Nishan, Burns, Alan R, Creighton, Chad J, Martinez, Elisabet, Zal, Tomasz, Grande-Allen, K Jane, Yamauchi, Mitsuo, Kurie, Jonathan
    J Clin Invest, 2015;125(3):1147-62.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human Lysine Hydroxylase 2/PLOD2 Antibody

Average Rating: 4 (Based on 1 Review)

5 Star
0%
4 Star
100%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Human Lysine Hydroxylase 2/PLOD2 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human Lysine Hydroxylase 2/PLOD2 Antibody
By Anonymous on 05/02/2016
Application: WB Sample Tested: MDA-MB-231 human breast cancer cell line Species: Human

western blot detection of PLOD2 in MDA-MB-231 transfected with siRNA against PLOD2. We routinely detect PLOD2 around 100KDa,this is likely related to post-translation modifications of PLOD2