Human M-CSF Antibody

Catalog # Availability Size / Price Qty
AF216
AF216-SP
Cell Proliferation Induced by M‑CSF and Neutralization by Human M‑CSF Antibody.
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Product Details
Citations (6)
FAQs
Supplemental Products
Reviews (2)

Human M-CSF Antibody Summary

Species Reactivity
Human
Specificity
Detects human M-CSF in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant mouse (rm) M‑CSF is observed, and less than 1% cross-reactivity with recombinant rat M-CSF, rmSCF, and recombinant human SCF is observed. 
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human M-CSF
Glu33-Ser190
Accession # NP_757350
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human M-CSF (Catalog # 216-MC)
Neutralization
Measured by its ability to neutralize M‑CSF-induced proliferation in the M‑NFS‑60 mouse myelogenous leukemia lymphoblast cell line. Halenbeck, R. et al. (1989) Biotechnology 7:710. The Neutralization Dose (ND50) is typically 0.01-0.03 µg/mL in the presence of 2.5 ng/mL Recombinant Human M‑CSF.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by M‑CSF and Neutralization by Human M‑CSF Antibody. View Larger

Cell Proliferation Induced by M‑CSF and Neutralization by Human M‑CSF Antibody. Recombinant Human M-CSF (Catalog # 216-MC) stimulates proliferation in the M-NFS-60 mouse myelogenous leukemia lymphoblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human M-CSF (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human M-CSF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF216). The ND50 is typically 0.01-0.03 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: M-CSF

M-CSF, also known as CSF-1, is a four-alpha -helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation. M-CSF is also essential for the survival and proliferation of osteoclast progenitors. M-CSF also primes and enhances macrophage killing of tumor cells and microorganisms, regulates the release of cytokines and other inflammatory modulators from macrophages, and stimulates pinocytosis. M-CSF increases during pregnancy to support implantation and growth of the decidua and placenta. Sources of M-CSF include fibroblasts, activated macrophages, endometrial secretory epithelium, bone marrow stromal cells and activated endothelial cells. The M-CSF receptor (c-fms) transduces its pleotropic effects and mediates its endocytosis. M-CSF mRNAs of various sizes occur. Full length human M-CSF transcripts encode a 522 amino acid (aa) type I transmembrane (TM) protein with a 464 aa extracellular region, a 21 aa TM domain, and a 37 aa cytoplasmic tail that forms a 140 kDa covalent dimer. Differential processing produces two proteolytically cleaved, secreted dimers. One is an N- and O- glycosylated 86 kDa dimer, while the other is modified by both glycosylation and chondroitin-sulfate proteoglycan (PG) to generate a 200 kDa subunit. Although PG-modified M-CSF can circulate, it may be immobilized by attachment to type V collagen. Shorter transcripts encode M-CSF that lacks cleavage and PG sites and produces an N-glycosylated 68 kDa TM dimer and a slowly produced 44 kDa secreted dimer. Although forms may vary in activity and half-life, all contain the N-terminal 150 aa portion that is necessary and sufficient for interaction with the M-CSF receptor. The first 223 aa of mature human M-CSF shares 88%, 86%, 81% and 74% aa identity with corresponding regions of dog, cow, mouse and rat M-CSF, respectively. Human M-CSF is active in the mouse, but mouse M-CSF is reported to be species-specific.

Long Name
Macrophage Colony Stimulating Factor
Entrez Gene IDs
1435 (Human); 12977 (Mouse)
Alternate Names
colony stimulating factor 1 (macrophage); CSF1; CSF-1; Lanimostim; macrophage colony stimulating factor; macrophage colony-stimulating factor 1; MCSF; M-CSF; MCSFlanimostim; MGC31930

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Citations for Human M-CSF Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Fucoidan modulates cytokine production and migration of THP-1-derived macrophages via colony-stimulating factor-1
    Authors: Peng Li, Huayang Wang, Qianqian Shao, Beihua Kong, Xun Qu
    Molecular Medicine Reports
  2. Crosstalk between macrophage-derived PGE2 and tumor UHRF1 drives hepatocellular carcinoma progression
    Authors: J Zhang, H Zhang, X Ding, J Hu, Y Li, J Zhang, H Wang, S Qi, A Xie, J Shi, M Xiang, Y Bin, G Wang, L Wang, Z Wang
    Theranostics, 2022-05-01;12(8):3776-3793.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: Neutralization, Western Blot
  3. Long non-coding RNA HOMER3-AS1 drives hepatocellular carcinoma progression via modulating the behaviors of both tumor cells and macrophages
    Authors: J Pu, W Li, A Wang, Y Zhang, Z Qin, Z Xu, J Wang, Y Lu, Q Tang, H Wei
    Cell Death & Disease, 2021-11-23;12(12):1103.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  4. Tumoral NOX4 recruits M2 tumor-associated macrophages via ROS/PI3K signaling-dependent various cytokine production to promote NSCLC growth
    Authors: J Zhang, H Li, Q Wu, Y Chen, Y Deng, Z Yang, L Zhang, B Liu
    Redox Biol, 2019-02-06;22(0):101116.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Neutralization
  5. Pleiotrophin produced by multiple myeloma induces transdifferentiation of monocytes into vascular endothelial cells: a novel mechanism of tumor-induced vasculogenesis.
    Authors: Chen H, Campbell RA, Chang Y, Li M, Wang CS, Li J, Sanchez E, Share M, Steinberg J, Berenson A, Shalitin D, Zeng Z, Gui D, Perez-Pinera P, Berenson RJ, Said J, Bonavida B, Deuel TF, Berenson JR
    Blood, 2008-12-05;113(9):1992-2002.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  6. LARRPM restricts lung adenocarcinoma progression and M2 macrophage polarization through epigenetically regulating LINC00240 and CSF1
    Authors: Yue Li, Chen Chen, Hai-lin Liu, Zhen-fa Zhang, Chang-li Wang
    Cellular & Molecular Biology Letters

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Reviews for Human M-CSF Antibody

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Human M-CSF Antibody
By Anonymous on 07/23/2019
Application: ELISA Sample Tested: EDTA Plasma Species: Human

Human M-CSF Antibody
By Anonymous on 11/03/2017
Application: ELISA Sample Tested: Serum and Plasma Species: Human

This goat poly clonal was biotinylated upon receipt. It was then subsequently used as a detection antibody in a sandwich ELISA with the R&D Systems monoclonal MAB616. This assay was used to measure human M-CSF in serum samples.