Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all MafF Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human MafF
Ser2-Ser164
Accession # Q9ULX9

Specificity

Detects human MafF in direct ELISAs and Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) MafG and rhMafK is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human MafF Antibody

Detection of Human MafF antibody by Western Blot.

Detection of Human MafF by Western Blot.

Western blot shows recombinant human (rh) MafF, MafG, and MafK (2 ng/lane). PVDF membrane was probed with 2 µg/mL Goat Anti-Human MafF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3917) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band for MafF was detected at approxi-mately 19 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human MafF antibody by Western Blot.

Detection of Human MafF by Western Blot.

Western blot shows lysates of MDA-MB-468 human breast cancer cell line and SK-Mel-28 human malignant melanoma cell line. PVDF membrane was probed with 2 µg/mL Goat Anti-Human MafF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3917) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band for MafF was detected at approximately 19 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

MafF antibody in HepG2 Human Cell Line by Immunocytochemistry (ICC).

MafF in HepG2 Human Cell Line.

MafF was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Goat Anti-Human MafF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3917) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (left panel, red; Catalog # NL001) and counterstained with DAPI (right panel, blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human MafF Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed HepG2 human hepatocellular carcinoma cell line

Western Blot

2 µg/mL
Sample: MDA-MB-468 human breast cancer cell line and SK-Mel-28 human malignant melanoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MafF

Maf family members form a unique subclass of basic-leucine zipper (bZIP) transcription factors. Maf proteins are subdivided into two groupings: large, including
c‑Maf, Nrl, MafA, and MafB; and small, including MafF, MafG, and MafK. Large Mafs contain an N-terminal acidic domain important for transcriptional activation that is lacking in small Maf family members. Small Maf/Nrf2 heterodimers have been implicated in the regulation of antioxidant response element-dependent genes.

Long Name

v-maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog F

Alternate Names

hMafF, transcription factor MafF, U-Maf, v-maf avian musculoaponeurotic fibrosarcoma oncogene family protein F, v-maf musculoaponeurotic fibrosarcoma (avian) oncogene family, protein F, V-maf musculoaponeurotic fibrosarcoma oncogene homolog F, v-maf musculoaponeurotic fibrosarcoma oncogene homolog F (avian)

Entrez Gene IDs

23764 (Human); 17133 (Mouse)

Gene Symbol

MAFF

UniProt

Q9ULX9

Additional MafF Products

Product Documents for Human MafF Antibody

Certificate of Analysis

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Product Specific Notices for Human MafF Antibody

For research use only

Citations for Human MafF Antibody

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Protocols

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