Human MANF Antibody

Catalog # Availability Size / Price Qty
AF3748
AF3748-SP
Detection of Human MANF by Western Blot.
3 Images
Product Details
Citations (5)
FAQs
Supplemental Products
Reviews (3)

Human MANF Antibody Summary

Species Reactivity
Human
Specificity
Detects human MANF in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human MANF
Leu22-Leu179
Accession # P55145
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below
Knockout Validated
MANF is specifically detected in HEK293T human embryonic kidney parental cell line but is not detectable in MANF knockout HEK293T cell line.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot View Larger

Detection of Human MANF by Western Blot. Western blot shows lysates of SH‑SY5Y human neuroblastoma cell line, U‑87 MG human glioblastoma/astrocytoma cell line, and Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human MANF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3748) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for MANF at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Knockout Validated Western Blot Shows Human MANF Antibody Specificity by Using Knockout Cell Line. View Larger

Western Blot Shows Human MANF Specificity by Using Knockout Cell Line. Western blot shows lysates of HEK293T human embryonic kidney parental cell line and MANF knockout HEK293T cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human MANF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3748) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for MANF at approximately 17 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293Tcell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MANF

Mesencephalic astrocyte-derived neurotrophic factor (MANF), also known as arginine-rich, mutated in early stage tumors (ARMET) and arginine-rich protein (ARP), is a 20 kDa member of the ARMET family of proteins (1). The name ARMET comes from the fact that the protein was initially thought to be 50 aa longer at the N‑terminus and to contain an arginine-rich region (2-5). The presence of a specific mutation changing the previously numbered codon 50 from ATG to AGG, or deletion of that codon, has been reported in a variety of solid tumors (2-4). Human MANF is synthesized as a 179 amino acid (aa) precursor that contains a 21 aa signal sequence and a 158 aa mature chain. Mature human MANF is 99%, 98%, and 96% aa identical to mature rat, mouse and bovine MANF, respectively. MANF is localized to the endoplasmic reticulum (ER) and Golgi apparatus, and is also secreted (5).  In the CNS, MANF selectively protects nigral dopaminergic neurons, versus GABAergic or serotonergic neurons, which suggests that MANF may be indicated for the treatment of Parkinson’s disease (1). MANF is also one of the 12 commonly unfolded protein response (UPR)-upregulated genes (5). One study showed that MANF plays an important role in protecting cells against tunicamycin and thapsigargin-induced cell death (5). Loss of MANF renders cells more susceptible to those drugs, but also increases cell proliferation and decreases cell size (5). Another study showed that MANF is an endoplasmic reticulum stress response (ERSR) gene in the heart that can be induced and secreted in response to ER stresses, including ischemia, and that extracellular MANF may protect cardiac myocytes in an autocrine and paracrine manner (6). 

References
  1. Petrova, P.S. et al. (2003) J. Mol. Neurosci. 20:173.
  2. Shridhar, V. et al. (1996) Oncogene 12:1931.
  3. Shridhar, R. et al. (1996) Cancer Res. 56:5576.
  4. Shridhar, V. et al. (1997) Oncogene 14:2213.
  5. Apostolou, A. et al. (2008) Exp. Cell Res. 314:2454.
  6. Tadimalla, A. et al. (2008) Circ. Res. 103:1249.
Long Name
Mesencephalic Astrocyte-derived Neurotrphic Factor
Entrez Gene IDs
7873 (Human); 74840 (Mouse); 315989 (Rat)
Alternate Names
Arginine-Rich Protein; arginine-rich, mutated in early stage tumors; ARMET; ARP; MANF; mesencephalic astrocyte-derived neurotrophic factor; Protein ARMET

Product Datasheets

Citations for Human MANF Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Human-Specific Regulation of Neurotrophic Factors MANF and CDNF by microRNAs
    Authors: J Konovalova, D Gerasymchu, SN Arroyo, S Kluske, F Mastroiann, AV Pereyra, A Domanskyi
    International Journal of Molecular Sciences, 2021;22(18):.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Capture
  2. Increased Serum Levels of Mesencephalic Astrocyte-Derived Neurotrophic Factor in Subjects With Parkinson's Disease
    Authors: E Galli, A Planken, L Kadastik-E, M Saarma, P Taba, P Lindholm
    Front Neurosci, 2019;13(0):929.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Capture
  3. Mesencephalic Astrocyte-Derived Neurotrophic Factor Is Upregulated with Therapeutic Fasting in Humans and Diet Fat Withdrawal in Obese Mice
    Authors: E Galli, J Rossi, T Neumann, JO Andressoo, S Drinda, P Lindholm
    Sci Rep, 2019;9(1):14318.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Capture
  4. Mesencephalic Astrocyte-derived Neurotrophic Factor Protects the Heart from Ischemic Damage and Is Selectively Secreted upon Sarco/endoplasmic Reticulum Calcium Depletion.
    Authors: Glembotski CC, Thuerauf DJ, Huang C
    J. Biol. Chem., 2012;287(31):25893-904.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Mesencephalic astrocyte-derived neurotrophic factor is an ischemia-inducible secreted endoplasmic reticulum stress response protein in the heart.
    Authors: Tadimalla A, Belmont PJ, Thuerauf DJ, Glassy MS, Martindale JJ, Gude N, Sussman MA, Glembotski CC
    Circ. Res., 2008;103(11):1249-58.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot

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Reviews for Human MANF Antibody

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Human MANF Antibody
By Anonymous on 03/11/2019
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human MANF Antibody
By Anonymous on 11/11/2018
Application: ELISA Sample Tested: Serum and Plasma Species: Human

Human MANF Antibody
By Anonymous on 11/02/2018
Application: Microarray Sample Tested: EDTA Plasma Species: Human