Human MBL Antibody

Catalog # Availability Size / Price Qty
AF2307
AF2307-SP
Product Details
Citations (6)
FAQs
Supplemental Products
Reviews (3)

Human MBL Antibody Summary

Species Reactivity
Human
Specificity
Detects human MBL in ELISAs and Western blots. In sandwich ELISAs, less than 0.05% cross-reactivity with recombinant mouse (rm) MBL and rmMBL‑2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human MBL
Glu21-Ile248
Accession # AAH96182
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human MBL (Catalog # 2307-MB)

Human MBL Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Human MBL Biotinylated Antibody (Catalog # BAF2307)

Standard: Recombinant Human MBL Protein (Catalog # 2307-MB)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MBL

MBL belongs to the collectin family of innate immune defense proteins, which bind to an array of carbohydrate patterns on pathogen surfaces. MBL homotrimerizes to form a structural unit that further associates into oligomeric structures. Circulating MBL is complexed with three MBL-associated serine proteases (MASPs). Human MBL shares approximately 60% amino acid sequence identity with mouse MBL1 or MBL2.

References
     
Long Name
Mannose Binding Lectin
Entrez Gene IDs
4153 (Human)
Alternate Names
COLEC1; COLEC1collectin-1; Collectin-1; HSMBPC; Mannan-binding protein; mannose-binding lectin (protein C) 2, soluble (opsonic defect); mannose-binding lectin (protein C) 2, soluble; Mannose-binding lectin; mannose-binding protein C; MBL; MBL2; MBL2D; MBLmannan-binding lectin; MBP; MBP1; MBP1mannose-binding lectin 2, soluble (opsonic defect); MBP-C; MGC116832; MGC116833

Product Datasheets

Citations for Human MBL Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
Filter your results:

Filter by:

  1. Mannan-Binding Lectin Suppresses Peptidoglycan-Induced TLR2 Activation and Inflammatory Responses
    Authors: F Wang, Y Li, C Yang, Y Mu, Y Wang, W Zhang, Y Yang, C Chen, S Song, Z Shen, W Wang, J Li, J Zhai, K Guo, R Sun, L Yu, M Wang
    Mediators Inflamm., 2019;2019(0):1349784.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture Treatment Control
  2. Moraxella catarrhalis Evades Host Innate Immunity via Targeting Cartilage Oligomeric Matrix Protein.
    Authors: Liu G, Gradstedt H, Ermert D, Englund E, Singh B, Su Y, Johansson M, Aspberg A, Agarwal V, Riesbeck K, Blom A
    J Immunol, 2016;196(3):1249-58.
    Species: Bacteria - Moraxella catarrhalis
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Mannan-binding lectin inhibits Candida albicans-induced cellular responses in PMA-activated THP-1 cells through Toll-like receptor 2 and Toll-like receptor 4.
    Authors: Wang M, Wang F, Yang J, Zhao D, Wang H, Shao F, Wang W, Sun R, Ling M, Zhai J, Song S
    PLoS ONE, 2013;8(12):e83517.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  4. A metalloproteinase karilysin present in the majority of Tannerella forsythia isolates inhibits all pathways of the complement system.
    Authors: Jusko M, Potempa J, Karim AY, Ksiazek M, Riesbeck K, Garred P, Eick S, Blom AM
    J. Immunol., 2012;188(5):2338-49.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development
  5. Scabies mite peritrophins are potential targets of human host innate immunity.
    Authors: Mika A, Goh P, Holt DC
    PLoS Negl Trop Dis, 2011;5(9):e1331.
    Species: Human
    Sample Types: Serum
    Applications: Western Blot
  6. Serglycin inhibits the classical and lectin pathways of complement via its glycosaminoglycan chains: implications for multiple myeloma.
    Authors: Skliris A, Happonen KE, Terpos E, Labropoulou V, Borset M, Heinegard D, Blom AM, Theocharis AD
    Eur. J. Immunol., 2011;41(2):437-49.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA Development

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human MBL Antibody

Average Rating: 4.3 (Based on 3 Reviews)

5 Star
33.33%
4 Star
66.67%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Human MBL Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human MBL Antibody
By Anonymous on 10/29/2019
Application: Simple Western Sample Tested: Serum Species: Human

Human serum analyzed by Simple Western under reducing conditions.


Human MBL Antibody
By Anonymous on 09/10/2019
Application: WB Sample Tested: Recombinant protein Species: Human

Human MBL Antibody
By Anonymous on 12/20/2018
Application: ELISA Sample Tested: EDTA Plasma Species: Human