Human MBL Antibody

R&D Systems | Catalog # AF2307

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Bacteria - Moraxella catarrhalis, Schistosoma mansoni

Applications

Validated:

Western Blot, ELISA Capture (Matched Antibody Pair)

Cited:

Western Blot, Neutralization, Flow Cytometry, Bioassay, ELISA Capture, ELISA Detection, ELISA Development

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all MBL Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human MBL
Glu21-Ile248
Accession # AAH96182

Specificity

Detects human MBL in ELISAs and Western blots. In sandwich ELISAs, less than 0.05% cross-reactivity with recombinant mouse (rm) MBL and rmMBL‑2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human MBL Antibody

Human MBL ELISA Standard Curve

Human MBL ELISA Standard Curve

Human MBL was serially diluted and captured by Goat Anti-Human MBL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2307) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human MBL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2307) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)
Human MBL ELISA Standard Curve

Human MBL ELISA Standard Curve

Human BMP-9 was serially diluted and captured by Goat Anti-Human MBL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2307) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human MBL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2307) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human MBL Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human MBL (Catalog # 2307-MB)

Human MBL Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 0.2-0.8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human MBL Biotinylated Antibody (Catalog # BAF2307)
  • Standard: Recombinant Human MBL Protein (Catalog # 2307-MB)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 3 reviews rated 4.3 using AF2307 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MBL

MBL belongs to the collectin family of innate immune defense proteins, which bind to an array of carbohydrate patterns on pathogen surfaces. MBL homotrimerizes to form a structural unit that further associates into oligomeric structures. Circulating MBL is complexed with three MBL-associated serine proteases (MASPs). Human MBL shares approximately 60% amino acid sequence identity with mouse MBL1 or MBL2.

References

     

Long Name

Mannose Binding Lectin

Alternate Names

COLEC1, Collectin-1, MBL2, MBL2D, MBP1

Entrez Gene IDs

4153 (Human)

Gene Symbol

MBL2

UniProt

AAH96182

Additional MBL Products

Product Documents for Human MBL Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human MBL Antibody

For research use only

Citations for Human MBL Antibody

Customer Reviews for Human MBL Antibody (3)

4.3 out of 5
3 Customer Ratings
5 Stars
33%
4 Stars
67%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • Human MBL Antibody
    Name: Anonymous
    Application: Simple Western
    Sample Tested: Serum
    Species: Human
    Verified Customer | Posted 10/29/2019
    Human serum analyzed by Simple Western under reducing conditions.
    Human MBL Antibody AF2307
  • Human MBL Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Recombinant protein
    Species: Human
    Verified Customer | Posted 09/10/2019
    Human MBL Antibody AF2307
  • Human MBL Antibody
    Name: Balaji Mahender
    Application: ELISA
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 12/20/2018

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