Human Meprin  alpha  Subunit/MEP1A Antibody

Catalog # Availability Size / Price Qty
AF3220
AF3220-SP

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Citations (3)
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Human Meprin  alpha  Subunit/MEP1A Antibody Summary

Species Reactivity
Human
Specificity
Detects human Meprin alpha Subunit/MEP1A in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross‑reactivity with recombinant mouse MEP1A and recombinant human MEP1B is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Meprin alpha Subunit/MEP1A
Val22-Gln601
Accession # AAA21338
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Meprin  alpha  Subunit/MEP1A (Catalog # 3220-ZN)
Immunohistochemistry
5-15 µg/mL
Immersion fixed paraffin-embedded sections of human intestine (jejunum)
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Meprin  alpha  Subunit/MEP1A (Catalog # 3220-ZN), see our available Western blot detection antibodies
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
HEK293 human embryonic kidney cell line fixed with paraformaldehyde and permeabilized with saponin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Meprin alpha Subunit/MEP1A

Meprins are multimeric proteases composed of alpha and beta  subunits, which are members of the astacin family of zinc endopeptidases (1, 2). Both subunits form disulfide‑linked homo- or heterooligomers, which are also referred to as Meprin A (composed of alpha  subunits with or without beta  subunits) and Meprin B (composed of beta  subunits only) (3). Although the two subunits share 42% identity in their amino acid sequence, they differ significantly in their oligomeric structure, post-translational processing and subsequently cellular location, and substrate and peptide bond specificity (4). The 746 amino acid sequence of human meprin alpha subunit precursor consists of a signal peptide (residues 1 to 21), a pro region (residues 22 to 65), and a mature chain (residues 66 to 746) containing following domains, catalytic (residues 62 to 263), MAM (residues 264 to 433), MATH (residues 434 to 593), EGF-like (residues 670 to 710), transmembrane (residues 713 to 740), and cytoplasmic (residues 741 to 746). The pro enzyme terminating at residue 601 was expressed and the secreted protein purified from conditioned medium. The molecular masses of rhMEP1A are similar to those observed for the alpha  subunit of rat Meprin A (5).

References
  1. Bond, J.S. and Beynon, R.J. (1995) Protein Sci. 4:1247.
  2. Stocker, W. et al. (1995) Protein Sci. 4:823.
  3. Bertenshaw, G.P. et al. (2001) J. Biol. Chem. 276:13248.
  4. Ishmael, F.T. et al. (2005) J. Biol. Chem. 280:13895.
  5. Bertenshaw, G.P. et al. (2003) J. Biol. Chem. 278:2522.
Entrez Gene IDs
4224 (Human); 17287 (Mouse); 25684 (Rat)
Alternate Names
bA268F1.1 (meprin A alpha (PABA peptide hydrolase)); EC 3.4.24; EC 3.4.24.18; endopeptidase-2; MEP1A; meprin A subunit alpha; meprin A, alpha (PABA peptide hydrolase); Meprin alpha Subunit; N-benzoyl-L-tyrosyl-P-amino-benzoic acid hydrolase subunit alpha; PABA peptide hydrolase; PPH alpha; PPHA

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Citations for Human Meprin  alpha  Subunit/MEP1A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Tracking cellular and molecular changes in a species-specific manner during experimental tumor progression in vivo
    Authors: E Indersie, KB Hooks, C Capdeviell, M Fabre, N Dugot-Sena, A Desplat, S Lepreux, A Merched, CF Grosset, M Hagedorn
    Oncotarget, 2018;9(22):16149-16162.
    Species: Human
    Sample Types: Complex Sample Type
    Applications: IHC
  2. Meprin ? contributes to collagen deposition in lung fibrosis
    Authors: V Biasin, M Wygrecka, LM Marsh, C Becker-Pau, L Brcic, B Ghanim, W Klepetko, A Olschewski, G Kwapiszews
    Sci Rep, 2017;7(0):39969.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  3. ADAM10 is the major sheddase responsible for the release of membrane-associated meprin A.
    Authors: Herzog C, Haun R, Ludwig A, Shah S, Kaushal G
    J Biol Chem, 2014;289(19):13308-22.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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