Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Intracellular Staining by Flow Cytometry, Immunoprecipitation, CyTOF-ready

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Meprin alpha Subunit/MEP1A Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Meprin alpha Subunit/MEP1A
Val22-Gln601
Accession # AAA21338

Specificity

Detects human Meprin alpha Subunit/MEP1A in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross‑reactivity with recombinant mouse MEP1A and recombinant human MEP1B is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human Meprin  alpha  Subunit/MEP1A Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human intestine (jejunum)

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Meprin  alpha  Subunit/MEP1A (Catalog # 3220-ZN), see our available Western blot detection antibodies

Intracellular Staining by Flow Cytometry

0.25 µg/106 cells
Sample: HEK293 human embryonic kidney cell line fixed with paraformaldehyde and permeabilized with saponin

Western Blot

0.1 µg/mL
Sample: Recombinant Human Meprin  alpha  Subunit/MEP1A (Catalog # 3220-ZN)

Reviewed Applications

Read 1 review rated 5 using AF3220 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Meprin alpha Subunit/MEP1A

Meprins are multimeric proteases composed of alpha and beta  subunits, which are members of the astacin family of zinc endopeptidases (1, 2). Both subunits form disulfide‑linked homo- or heterooligomers, which are also referred to as Meprin A (composed of alpha  subunits with or without beta  subunits) and Meprin B (composed of beta  subunits only) (3). Although the two subunits share 42% identity in their amino acid sequence, they differ significantly in their oligomeric structure, post-translational processing and subsequently cellular location, and substrate and peptide bond specificity (4). The 746 amino acid sequence of human meprin alpha subunit precursor consists of a signal peptide (residues 1 to 21), a pro region (residues 22 to 65), and a mature chain (residues 66 to 746) containing following domains, catalytic (residues 62 to 263), MAM (residues 264 to 433), MATH (residues 434 to 593), EGF-like (residues 670 to 710), transmembrane (residues 713 to 740), and cytoplasmic (residues 741 to 746). The pro enzyme terminating at residue 601 was expressed and the secreted protein purified from conditioned medium. The molecular masses of rhMEP1A are similar to those observed for the alpha  subunit of rat Meprin A (5).

References

  1. Bond, J.S. and Beynon, R.J. (1995) Protein Sci. 4:1247.
  2. Stocker, W. et al. (1995) Protein Sci. 4:823.
  3. Bertenshaw, G.P. et al. (2001) J. Biol. Chem. 276:13248.
  4. Ishmael, F.T. et al. (2005) J. Biol. Chem. 280:13895.
  5. Bertenshaw, G.P. et al. (2003) J. Biol. Chem. 278:2522.

Alternate Names

MEP1A, PPHA

Entrez Gene IDs

4224 (Human); 17287 (Mouse); 25684 (Rat)

Gene Symbol

MEP1A

UniProt

AAA21338

Additional Meprin alpha Subunit/MEP1A Products

Product Documents for Human Meprin  alpha  Subunit/MEP1A Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Meprin  alpha  Subunit/MEP1A Antibody

For research use only

Related Research Areas

Metalloproteases and Regulators

Citations for Human Meprin  alpha  Subunit/MEP1A Antibody

Customer Reviews for Human Meprin  alpha  Subunit/MEP1A Antibody (1)

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  • Human Meprin  alpha  Subunit/MEP1A Antibody
    Name: Jessica Ong
    Application: Western blot and immunofluorescence
    Sample Tested: C57BL/6J urine and C57BL/6J kidney
    Species: Mouse
    Verified Customer | Posted 08/20/2019
    Mouse kidney tissue and mouse urine western blot. Immunofluorescence of frozen mouse tissue 1:40
    Western blot 1:1000, IF 1:40
    Human Meprin  alpha  Subunit/MEP1A Antibody AF3220

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